A5058075551- Microbial Metabolic Engineering and Bioproduction
- Genetics, Aging, and Longevity in Model Organisms
- Tryptophan and brain disorders
- Mycobacterium research and diagnosis
- Biochemical Acid Research Studies
- Gene Regulatory Network Analysis
- Neuroscience and Neuropharmacology Research
- Genetic Associations and Epidemiology
- Birth, Development, and Health
- Health disparities and outcomes
- Bioinformatics and Genomic Networks
- Tuberculosis Research and Epidemiology
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Diagnosis and treatment of tuberculosis
- Health, Environment, Cognitive Aging
- Metabolomics and Mass Spectrometry Studies
- Chronic Disease Management Strategies
- Distributed and Parallel Computing Systems
- Biofuel production and bioconversion
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Carbon Nanotubes in Composites
- Scientific Computing and Data Management
- Nanotechnology research and applications
University of Zurich
2022-2024
University at Buffalo, State University of New York
2019-2022
Columbia University
2022
University of Wisconsin–Madison
2022
Florida State University
2022
Emory University
2022
ETH Zurich
2019-2021
Institute for Biomedical Engineering
2020-2021
Bioengineering (Switzerland)
2020-2021
École Polytechnique Fédérale de Lausanne
2016
Abstract Understanding the emerging models of adaptive resistance is key to overcoming cancer chemotherapy failure. Using human breast explants, in vitro cell lines, mouse vivo studies and mathematical modelling, here we show that exposure a taxane induces phenotypic state transition towards favoured transient CD44 Hi CD24 chemotherapy-tolerant state. This associated with clustering membrane lipid rafts, leading activation Src Family Kinase (SFK)/hemopoietic kinase (Hck) suppression...
To uncover underlying mechanisms associated with failure of indoleamine 2,3-dioxygenase 1 (IDO1) blockade in clinical trials, we conducted a pilot, window-of-opportunity study 17 patients newly diagnosed advanced high-grade serous ovarian cancer before their standard tumor debulking surgery. Patients were treated the IDO1 inhibitor epacadostat, and immunologic, transcriptomic, metabolomic characterization microenvironment was undertaken baseline posttreatment biopsies. inhibition resulted...
Alzheimer’s disease (AD) is the most common neurodegenerative affecting elderly worldwide. Mitochondrial dysfunction has been proposed as a key event in etiology of AD. We have previously modeled amyloid-beta (Aβ)-induced mitochondrial transgenic Caenorhabditis elegans strain by expressing human Aβ peptide specifically neurons (GRU102). Here, we focus on deeper metabolic changes associated with this Aβ-induced dysfunction. Integrating metabolomics, transcriptomics and computational modeling,...
Genome-scale metabolic models (GEMs) provide a powerful framework for simulating the entire set of biochemical reactions in cell using constraint-based modeling strategy called flux balance analysis (FBA). FBA relies on an assumed objective generating fluxes GEMs. But, most appropriate is not always obvious given condition and likely context-specific, which often complicate estimation alterations between conditions. Here, we propose new method, ΔFBA (deltaFBA), that integrates differential...
When human cord blood–derived CD34+ cells are induced to differentiate, they undergo rapid and dynamic morphological molecular transformations that critical for fate commitment. In particular, the pass through a transitory phase known as “multilineage-primed” state. These characterized by mixed gene expression profile, different in each cell, with coexpression of many genes characteristic concurrent cell lineages. The aim our study is understand mechanisms establishment exit from this We...
Abstract Disparities in socio-economic status (SES) predict many immune system-related diseases, and previous research documents relationships between SES the cell transcriptome. Drawing on a bioinformatically-informed network approach, we situate these findings broader molecular framework by examining upstream regulators of SES-associated transcriptional alterations. Data come from National Longitudinal Study Adolescent to Adult Health (Add Health), nationally representative sample 4543...
Many common chronic diseases of aging are negatively associated with socioeconomic status (SES). This study examines whether inequalities can already be observed in the molecular underpinnings such 30s, before many them become prevalent. Data come from National Longitudinal Study Adolescent to Adult Health (Add Health), a large, nationally representative sample US subjects who were followed for over two decades beginning adolescence. We now have transcriptomic data (mRNA-seq) random subset...
IS6110 sequence based polymerase chain reaction (PCR) was compared with conventional bacteriological techniques in the laboratory diagnosis of extra-pulmonary tuberculosis (EPTB). One hundred and ninety one non-repeated clinical samples EPTB 17 from non-tuberculous cases as controls were included. All processed for Ziehl-Neelsen staining acid fast bacilli (AFB) 143 by culture M. tuberculosis. PCR amplification primers targeting 123 bp fragment insertion element complex. Of total 191...
Abstract Alzheimer’s disease (AD) is the most common neurodegenerative affecting elderly worldwide. Mitochondrial dysfunction has been proposed as a key event in etiology of AD. We have previously modeled amyloid-beta (Aβ)-induced mitochondrial transgenic Caenorhabditis elegans strain by expressing human Aβ peptide specifically neurons (GRU102). Here, we focus on deeper analysis these metabolic changes associated with Aβ-induced dysfunction. Integrating metabolomics, transcriptomics,...
A total of 26 clinically diagnosed adult patients, with active untreated lepromatous leprosy, a Bacteriological Index 4+ or more, were admitted to the hospital Central Leprosy Teaching and Research Institute, Chengalpattu, India, between 1989 1991.After prescribed inves tigations, patients randomly allocated in groups 3 treatment regimens, namely: 1, clofazimine 50 mg daily 300 once 4 weeks + dapsone 100 (AA); 2, (AA)+ofioxacin 400 (BB); 3, 800 (CC).The drugs administered for 56 days...
Abstract Genome-scale metabolic models (GEMs) provide a powerful framework for simulating the entire set of biochemical reactions occurring in cell. Constraint-based modeling tools like flux balance analysis (FBA) developed purposes predicting distribution using GEMs face considerable difficulties estimating alterations between experimental conditions. Particularly, most appropriate objective FBA is not always obvious, likely context-specific, and necessarily same Here, we propose new...
Summary Extra-cellular accumulation of Amyloid-β (Aβ) plaques is causatively associated with Alzheimer’s disease (AD). However, mechanisms that mediate the pre-pathological state amyloid plaque formation remain elusive. Here, using paired RNAi and kinase inhibitor screens, we discovered AKT-mediated insulin/nutrient signaling suppresses lysosomal clearance Aβ promotes formation. This mechanism cell-autonomous functions in multiple systems, including iPSC-derived human neurons vivo. Nutrient...
Semiconducting Single-Walled Carbon Nanotubes (SWCNTs) have a unique combination of optical, chemical, and physical properties that render them suitable for broad range applications 1–3 . Since SWCNTs are composed exclusively surface atoms, the fluorescence emission nanotubes is very sensitive to surrounding environment, enabling single-molecule detection limits especially desirable biosensing applications. The selectivity SWCNT-based sensors can be tuned by engineering polymer wrapping...
Abstract Disparities in socio-economic status (SES) predict many immune system-related diseases, and previous research documents relationships between SES the cell transcriptome. Drawing on a bioinformatically-informed network approach, we situate these findings broader molecular framework by examining upstream regulators of SES-associated transcriptional alterations. Data come from National Longitudinal Study Adolescent to Adult Health (Add Health), nationally representative sample 4,543...
Summary When human cord blood derived CD34+ cells are induced to differentiate in vitro , they undergo rapid and dynamic morphological molecular transformations that critical for fate commitment. Using ATAC-seq single-cell RNA sequencing, we detected two phases this process. In the first phase, observed a global chromatin opening makes most of gene promoters genome accessible, followed by widespread upregulation transcription concomitant increase cell-to-cell variability expression. The...