A5059563268- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
- Genetic Syndromes and Imprinting
- Prenatal Screening and Diagnostics
- Cell Image Analysis Techniques
- T-cell and B-cell Immunology
- Bioinformatics and Genomic Networks
- Virus-based gene therapy research
- Gene expression and cancer classification
- Pharmacogenetics and Drug Metabolism
- CRISPR and Genetic Engineering
- Microfluidic and Bio-sensing Technologies
- Hematopoietic Stem Cell Transplantation
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- CAR-T cell therapy research
- Evolution and Genetic Dynamics
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Cancer Genomics and Diagnostics
- Chromosomal and Genetic Variations
- Pancreatic function and diabetes
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
École Pratique des Hautes Études
2014-2024
Inserm
2012-2024
Université Paris Sciences et Lettres
2016-2024
Sorbonne Université
2002-2024
Institut Carnot ARTS
2024
Assistance Publique – Hôpitaux de Paris
2024
Hôpital Saint-Antoine
2024
Centre de Recherche Saint-Antoine
2020-2023
University at Buffalo, State University of New York
2019
Genethon (France)
2006-2018
Distinct cell populations with regenerative capacity have been reported to contribute myofibres after skeletal muscle injury, including non-satellite cells as well myogenic satellite cells. However, the relative contribution of these distinct types repair and homeostasis identity adult stem remain unknown. We generated a model for conditional depletion by expressing human diphtheria toxin receptor under control murine Pax7 locus. Intramuscular injection during homeostasis, or combined injury...
DNA methylation patterns were evaluated during preimplantation mouse development by analyzing the binding of monoclonal antibody to 5-methylcytosine (5-MeC) on metaphase chromosomes. Specific chromosome observed in each cell stage. A banding pattern predominated chromosomes at one-cell Banding was replaced two-cell stage an asymmetrical labeling sister chromatids. Then, proportion decreased one-half division until blastocyst stage, and became progressively symmetrical weakly labeled. Our...
There is increasing evidence in both plants and animals that epigenetic marks are not always cleared between generations. Incomplete erasure at genes associated with a measurable phenotype results unusual patterns of inheritance from one generation to the next, termed transgenerational inheritance. The Agouti viable yellow (A(vy)) allele best-studied example this phenomenon mice. A(vy) result retrotransposon insertion upstream gene. Expression locus controlled by long terminal repeat (LTR)...
ABSTRACT In the present study, we have examined origin and growth pattern of β cells in pancreatic islets, to determine whether a single progenitor cell gave rise all precursors or if each few is founder different islet, islet mixture originating from pool cells. Aggregation mouse chimaeras where derived embryo can be identified islets on histological sections were analyzed. two chimaeras, contained both aggregated embryo. This clearly demonstrates that resulted several independent addition,...
In somatic tissues, the CpG island of imprinted Peg1/Mest gene is methylated on maternal allele. We have examined methylation and non-CpG sites this differentially in freshly ovulated oocytes, vitro aged preimplantation embryos. The pattern was heterogeneous despite fact that they all were arrested metaphase II. After short culture, became hypermethylated, whereas prolonged culture resulted demethylation a fraction oocytes. Non-CpG also occurred stage-specific manner. On alleles fully at...
The spontaneous emergence of phenotypic heterogeneity in clonal populations mammalian cells vitro is a rule rather than an exception. We consider two simple, mutually non-exclusive models that explain the generation diverse cell types homogeneous population. In first model, switch consequence extrinsic factors. Initially identical may become different because they encounter local environments induce adaptive responses. According to second intrinsic occur even environments.We have...
There was an error published in Development138, 3647-3656.The panel labels on the left indicating genotypes were misaligned Fig. 5A. The corrected 5 appears full below.The authors apologise to readers for this mistake.
Individual cells take lineage commitment decisions in a way that is not necessarily uniform. We address this issue by characterising transcriptional changes cord blood-derived CD34+ at the single-cell level and integrating data with cell division history morphological determined time-lapse microscopy. show major leading to multilineage-primed gene expression state occur very rapidly during first cycle. One of 2 stable lineage-primed patterns emerges gradually each variable timing. Some reach...
Recent studies demonstrated that cytosine methylation in the genome can be reversed without DNA replication by enzymatic mechanisms based on base excision-repair pathways. Both and demethylation are active cell nucleus at same time. One hypothesize actual level of CpG could result a balance between two antagonistic processes with rapid turnover. In present study, we used mass spectrometry to measure total methyl-cytosine content cultured human cells after short incubation known...
Despite the stochastic noise that characterizes all cellular processes cells are able to maintain and transmit their daughter stable level of gene expression. In order better understand this phenomenon, we investigated temporal dynamics expression variation using a double reporter model. We compared cell clones with transgenes coding for highly mRNA fluorescent proteins expressing destabilized mRNA-s proteins. Both types displayed strong heterogeneity levels. However, products produced...
Sex of early bovine embryos was determined by polymerase chain reaction (PCR) using a single blastomere removed at the 16–32 cell stage. Embryos were produced in vitro and biopsied on fifth day after fertilization. Biopsied cultured cumulus monolayer until embryo transfer. For PCR, one pair bovine-specific Y-chromosome-specific primers used. Definite signals following PCR amplification obtained 95.4% cases indicating that from preimplantation is sufficient for sex determination PCR. Nineteen...
ABSTRACT The microfilament inhibitor cytochalasin D inhibits extrusion of the first polar body when present during meiotic division mouse oocytes; however, it does not interfere with anaphase movement chromosomes, and thus induces formation tetraploid oocytes. After separation chromosomes in anaphase, two spindles start to assemble. However, they merge rapidly a single spindle forms. During transition between metaphase I II, presence D, drop histone kinase activity takes place demonstrating...
ABSTRACT Parthenogenetically activated BCF1 and fertilized BALB/c embryos were aggregated to form chimaeras. The fate of the parthenogenetic component was followed in conceptus during second half gestation. results indicate an early strong selection against cells extra-embryonal part, which is presumably complete by term, a weaker selective process embryo. During development, have nearly normal developmental potency embryo, allows their balanced contribution chimaeras on day 12. Later, this...
When human cord blood–derived CD34+ cells are induced to differentiate, they undergo rapid and dynamic morphological molecular transformations that critical for fate commitment. In particular, the pass through a transitory phase known as “multilineage-primed” state. These characterized by mixed gene expression profile, different in each cell, with coexpression of many genes characteristic concurrent cell lineages. The aim our study is understand mechanisms establishment exit from this We...
Abstract Background Cell differentiation requires the integration of two opposite processes, a stabilizing cellular memory, especially at transcriptional scale, and burst gene expression variability which follows induction. Therefore, actual capacity cell to undergo phenotypic change during process relies upon modification in this balance favors change-inducing variability. However, there are no experimental data providing insight on how fast transcriptomes identical cells would diverge...
Single-cell studies have demonstrated the presence of significant cell-to-cell heterogeneity in gene expression. Whether such is only a bystander or has functional role cell differentiation process still hotly debated.