A5053243722- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
- Neurofibromatosis and Schwannoma Cases
- Birth, Development, and Health
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Sarcoma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Health, Environment, Cognitive Aging
- Molecular Biology Techniques and Applications
- Air Quality and Health Impacts
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- Nutritional Studies and Diet
- Energy and Environment Impacts
- RNA modifications and cancer
- Chromatin Remodeling and Cancer
- Soft tissue tumor case studies
- Metabolism and Genetic Disorders
- Hippo pathway signaling and YAP/TAZ
- Frailty in Older Adults
- Genetic Associations and Epidemiology
- Circadian rhythm and melatonin
- Carcinogens and Genotoxicity Assessment
- Advanced biosensing and bioanalysis techniques
University of Utah
2014-2023
Intermountain Medical Center
2013-2018
Institute of Human Genetics
2003-2014
National Cancer Institute
2012-2014
Changhua Christian Hospital
2014
Leidos (United States)
2014
University of Pennsylvania
2014
Herlev Hospital
2011-2013
University of Copenhagen
2011-2013
Copenhagen University Hospital
2011-2013
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed hybridize the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a pair that eliminates this problem, allowing simple rapid measurement of closed tube, fluorescence-based assay. This assay will facilitate investigations biology roles they play molecular pathophysiology diseases aging.
Numerous studies demonstrate links between chronic stress and indices of poor health, including risk factors for cardiovascular disease poorer immune function. Nevertheless, the exact mechanisms how gets "under skin" remain elusive. We investigated hypothesis that impacts health by modulating rate cellular aging. Here we provide evidence psychological stress--both perceived chronicity stress--is significantly associated with higher oxidative stress, lower telomerase activity, shorter...
The current quantitative polymerase chain reaction (QPCR) assay of telomere length measures (T) signals in experimental DNA samples one set wells, and single copy gene (S) separate comparison to a reference DNA, yield relative T/S ratios that are proportional average length. Multiplexing this is desirable, because variation the amount pipetted would no longer contribute T/S, since T S be collected within each reaction, from same input DNA. also increases throughput lowers costs, half as many...
Telomere length (TL) is emerging as a biomarker for aging and survival. To evaluate factors influencing this trait, we measured TL in large homogeneous population, estimated the heritability (h(2)), tested parental effects on variation. Our sample included 356 men 551 women, aged 18-92 years, from Amish families. Mean leukocytes was by quantitative PCR (mean: 6,198 +/- 1,696 bp). The h(2) of 0.44 0.06 (P < 0.001), after adjusting age, sex, assay batch. As expected, negatively correlated with...
Background Leukocyte telomere length (LTL) is an emerging marker of biological age. Chronic inflammatory activity commonly proposed as a promoter aging in general, and leukocyte shortening particular. In addition, senescent cells with critically short telomeres produce pro-inflammatory factors. However, spite the causal links between LTL, there little clinical evidence support their covariation interaction. Methodology/Principal Findings To address this issue, we examined if individuals high...
Telomere length (TL) has been proposed as a marker of mitotic cell age and general index human organismic aging. Short absolute leukocyte telomere linked to cardiovascular-related morbidity mortality. Our aim was test whether the rate change in TL is related mortality healthy elderly cohort. We examined subsample 236 randomly selected Caucasian participants from MacArthur Health Aging Study (aged 70 79 years). DNA samples baseline 2.5 years later were assayed for mean leukocytes. Percent...
Recent meta-analyses have suggested that short telomere length was associated with increased risk of cancer. We therefore tested the hypotheses cancer and early death after measured leukocyte in a prospective study 47 102 Danish general population participants from Copenhagen City Heart Study General Population Study. Participants were followed for up to 20 years diagnosis death. Follow-up 100% complete. All statistical tests two-sided. Telomere decreased linearly increasing age (P <.001)....
Although telomere length (TL) is known to play a critical role in cellular senescence, the relationship of TL aging and longevity humans not well understood. In large biracial population-based cohort, we tested hypotheses that elderly persons with shorter peripheral white blood cells have poorer survival, life span, fewer years healthy (YHL). Associations were evaluated using Cox proportional hazard models linear regression analyses where appropriate. (in kilo base pairs) was associated...
Telomere length in humans is emerging as a biomarker of aging because its shortening associated with aging-related diseases and early mortality. However, genetic mechanisms responsible for these associations are not known. Here, cohort Ashkenazi Jewish centenarians, their offspring, offspring-matched controls, we studied the inheritance maintenance telomere variations two major genes telomerase enzyme activity, hTERT hTERC . We demonstrated that centenarians offspring maintain longer...
Objective— We tested the hypothesis that short telomere length is associated with increased risk of myocardial infarction, ischemic heart disease, and early death. Methods Results— measured leukocyte in 2 prospective studies 19 838 Danish general population participants from Copenhagen City Heart Study General Population Study. Participants were followed for up to years incident infarction (n=929), disease (n=2038), death (n=4342). Follow-up was 100% complete. Telomere decreased linearly...
Telomere shortening has been proposed as a marker of biological aging. Whether leukocyte telomere length is associated with mortality among patients stable coronary artery disease (CAD) unknown.We measured in 780 CAD prospective cohort study. Participants were categorized by quartiles length. Hazard Ratios (HRs) and 95% confidence intervals calculated for all-cause mortality, heart failure (HF) hospitalization, cardiovascular (CV) events. After 4.4 years follow-up there 166 deaths. Compared...
Abstract Obesity and weight gain in adulthood are associated with an increased risk of several cancers. Telomeres play a critical role maintaining genomic integrity may be involved carcinogenesis. Using data from 647 women ages 35 to 74 years the United States Puerto Rico (2003-2004), we examined association between current past anthropometric characteristics telomere length blood. In multivariate linear regression model, higher body mass index (BMI) hip circumference were inversely length....
Abstract Telomeres are repetitive DNA sequences that cap and protect the ends of chromosomes; critically short telomeres may lead to cellular senescence or carcinogenic transformation. Previous findings suggest a link between psychosocial stress, shorter telomeres, chronic disease risk. This cross-sectional study examined relative telomere length in relation perceived stress urinary hormones sample participants (n = 647) National Institute Environmental Health Sciences Sister Study, cohort...
Human age-dependent telomere attrition and shortening are associated with several age-associated diseases poorer overall survival. The aim of this study was to determine longitudinal leucocyte length dynamics identify factors temporal changes in length.Leucocyte measured by quantitative polymerase chain reaction 8074 participants from the Prevention Renal Vascular End-stage Disease (PREVEND) study, an ongoing community-based prospective cohort initiated 1997. Follow-up data were available at...