A5048991125- Cutaneous lymphoproliferative disorders research
- CAR-T cell therapy research
- T-cell and Retrovirus Studies
- Immunotherapy and Immune Responses
- Lymphoma Diagnosis and Treatment
- Fungal Infections and Studies
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Nail Diseases and Treatments
- Plant Pathogens and Fungal Diseases
- T-cell and B-cell Immunology
- Cutaneous Melanoma Detection and Management
- Single-cell and spatial transcriptomics
- Invertebrate Immune Response Mechanisms
- Genetic and rare skin diseases.
- Viral-associated cancers and disorders
- Histone Deacetylase Inhibitors Research
- Aquaculture disease management and microbiota
- Toxin Mechanisms and Immunotoxins
- IL-33, ST2, and ILC Pathways
- Melanoma and MAPK Pathways
- Inflammasome and immune disorders
- Pancreatic and Hepatic Oncology Research
- Autoimmune and Inflammatory Disorders Research
- Skin and Cellular Biology Research
University of Lausanne
2020-2025
University Hospital of Lausanne
2023-2024
University Hospital of Zurich
2016-2021
University of Zurich
2016-2021
Brigham and Women's Hospital
2021
Harvard University
2021
National Taiwan University
2010-2013
Abstract Background Fungal infections are the most frequent dermatoses. The gold standard treatment for dermatophytosis is squalene epoxidase (SQLE) inhibitor terbinafine. Pathogenic dermatophytes resistant to terbinafine an emerging global threat. Here, we determine proportion of fungal skin infections, analyse molecular mechanisms resistance, and validate a method its reliable rapid identification. Methods Between 2013 2021, screened 5634 consecutively isolated Trichophyton antifungal...
Patients with Sézary syndrome (SS), a leukemic variant of cutaneous T-cell lymphoma (CTCL), are prone to Staphylococcus aureus infections and have poor prognosis due treatment resistance. Here, we report that S staphylococcal enterotoxins (SE) induce drug resistance in malignant T cells against therapeutics commonly used CTCL. Supernatant from patient-derived, SE-producing recombinant SE significantly inhibit cell death induced by histone deacetylase (HDAC) inhibitor romidepsin primary...
A novel B cell subset that produces proangiogenic cytokines is increased in pathologies associated with angiogenesis.
Sézary syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphoma (L-CTCL) that arises from malignant clonally derived skin-homing CD4+ T cells. Based on advancements in our understanding the mechanisms underlying L-CTCL, boosting suppressed immune response emerges as a promising strategy SS management. Immune checkpoint inhibitory molecules have already demonstrated efficacy wide spectrum malignancies. Currently, agents targeting programmed death-1 (PD-1) axis are under...
Darier disease (DD) is a rare, inherited multi-organ disorder associated with mutations in the ATP2A2 gene. DD patients often have skin involvement characterized by malodorous, inflamed and recurrent, severe infections. Therapeutic options are limited inadequate for long-term management of this chronic disease. The aim study was to characterize cutaneous immune infiltrate lesions detail identify new therapeutic targets. Using gene protein expression profiling assays including scRNA...
Trichophyton indotineae is an emerging dermatophyte that causes severe tinea corporis and cruris. Numerous cases of terbinafine- azole-recalcitrant T. indotineae-related dermatophytosis have been observed in India over the past decade, are now being recorded worldwide. Whole genome sequencing three azole-resistant strains revealed a variable number repeats 2,404 base pair (bp) sequence encoding TinCYP51B tandem specifically at CYP51B locus position. However, many other resistant...
Abstract Cancer-associated immune dysfunction is a major challenge for effective therapies. The emergence of antibodies targeting tumor cell-surface antigens led to advancements in the treatment hematopoietic malignancies, particularly blood cancers. Yet their impact constrained against tumors origin manifesting skin. In this study, we employ clonality-supervised deep learning methodology dissect key pathological features implicated mycosis fungoides, most common cutaneous T-cell lymphoma....
Background. The outcome of patients with adenocarcinoma the esophagogastric junction (AEG) remains poor. programmed cell-death-protein-1 (PD-1), a co-inhibitory receptor primarily expressed by T-cells, represents potential new therapeutic target. PD-1, PD-1 ligand 1 (PD-L1), and PD-L2 expression have all been described as prognostic factors in variety cancers. Their patterns AEG, however, are poorly understood. We analyzed PD-L1, tumor-infiltrating lymphocytes (TILs) cancer-cells...
Despite novel therapeutic options, the long-term management of cutaneous T cell lymphoma (CTCL) remains challenging. Extracorporeal photopheresis (ECP) is an immunomodulating photochemotherapy associated with higher overall survival when used for treatment leukemic forms CTCL. Its exact mode action not fully elucidated. Immunogenic death (ICD) pivotal in cancer immunotherapy, marked by release damage-associated molecular patterns that enhance dendritic (DC) maturation and cytotoxic...
In Sézary syndrome (SS) impaired T-cell function and cytokine profile lead to immune evasion. Immune checkpoints non-redundantly regulate responses targeting them is promising. We evaluated the expression of BTLA, CTLA-4, FCRL3, LAG-3, TIGIT in tumor non-tumor SS T-cells.Compared CD4+ T helper cells from ten healthy individuals, eight patients had a significant upregulation BTLA (1.5-fold; p < .0001), FRCL3 (2.2-fold; .0028) .0003) expression. contrast, we found reduced LAG-3+ blood...
The application of machine learning approaches to imaging flow cytometry (IFC) data has the potential transform diagnosis hematological diseases. However, need for manually labeled single-cell images model training severely limited its clinical application. To address this, we present iCellCnn, a weakly supervised deep approach label-free IFC-based blood diagnostics. We demonstrate capability iCellCnn achieve Sézary syndrome (SS) from patient samples on basis bright-field IFC T cells...
Rapid and reliable fungal identification is crucial to delineate infectious diseases, establish appropriate treatment for onychomycosis. Compared conventional diagnostic methods, molecular techniques are faster feature higher accuracy in identification. However, current clinical practice, mycology not widely available, its practical applicability still under discussion. This study summarizes the results of 16,094 consecutive nail specimens with suspicion We performed PCR/sequencing on all...
Pulmonary metastasectomy (PM) is routinely performed in colorectal cancer (CRC) patients with oligometastatic spreading to the lungs. Patients an aggressive tumor phenotype should be excluded from PM, since its benefit outweighed by early recurrence and impaired prognosis. Expression of PD-1 ligands are prognostic factors a variety primary tumors. However, their impact on patients' outcome setting PM for CRC has not been evaluated before.53 pulmonary metastases receiving curative intent were...
Astakine is an important cytokine that involved in crustacean hematopoiesis. Interestingly, the protein levels of astakine increased dramatically plasma LPS-injected shrimp while mRNA remained unchanged. Here, we investigated involvement 3′-untranslated region (UTR) its expression. The 3′-UTR down-regulated expression reporter but stability gene was unaffected. We identified functional regulatory elements 3′-UTR, where 3′-UTR242–483 acted as repressor. electrophoresis mobility shift assay...
Abstract Background Extracorporeal photopheresis (ECP) has emerged as a systemic first-line immunomodulatory therapy in leukaemic cutaneous T-cell lymphoma (L-CTCL) and is now beginning to be utilized other T-cell-mediated diseases. Although ECP been used for nearly 30 years, its mechanisms of action are not sufficiently understood, biomarkers response scarce. Objectives We aimed investigate the effects on cytokine secretion patterns patients with L-CTCL, help elucidate mechanism action....
Mycosis fungoides (MF) is a type of cutaneous T-cell lymphoma. Chlormethine (CL) recommended as first-line therapy for MF, with major purpose to kill tumor cells through DNA alkylation. To study the extent treatment susceptibility and specificity, we investigated gene expression different repair pathways, double-stranded breaks, cell proliferation clonal TCR Vβ+ populations in lymphoma skin on direct exposure CL. Healthy human T were less susceptible CL than two T-lymphoma lines, resulting...
Schnitzler syndrome is a rare autoinflammatory disease, which defined by the presence of two major criteria: chronic urticaria and monoclonal immunoglobulin M (IgM) or G gammopathy, in combination with at least additional minor recurrent fever, leukocytosis and/or elevated C-reactive protein (CRP), objective signs abnormal bone remodelling neutrophilic infiltrate skin biopsy. We report on 68-year-old female patient 10-year medical history urticaria, severe arthralgia increased CRP. Over...
Histone deacetylase (HDAC) inhibitors, approved for the treatment of cutaneous T-cell lymphoma (CTCL), are non-selective agents associated with an unsatisfactory response and considerable side-effects. Targeting single HDAC isoforms is considered to provide novel therapeutic options. HDAC6 overexpressed in primary samples from patients CTCL preclinical studies using transgenic mice that spontaneously develop a CTCL-like disease, have suggested combinations including inhibitors may be...
SummarySézary syndrome (SS) is a rare, leukemic type of cutaneous T-cell lymphoma (CTCL), for which extracorporeal photopheresis (ECP) first-line therapy. Reliable biomarkers to objectively monitor the response ECP in patients with SS are missing. We examined quantitative and qualitative impact on natural killer (NK) cell activity patients, especially their functional ability antibody-dependent cell-mediated cytotoxicity (ADCC). Further, we addressed question whether magnitude effect ADCC...
The BGLF4 protein of Epstein–Barr virus (EBV) is a serine/threonine kinase that phosphorylates several viral and cellular substrates at cyclin-dependent target sites. required for efficient DNA replication release mature virions. It also stimulates the transactivation activity immediate-early transactivator Zta (BZLF1) suppresses activities BMRF1 EBNA-2. This study aimed to characterize further regulation expression transcriptional translational levels. was shown expressed with early...
Efficient vaccination can be achieved by injections of in vitro transcribed mRNA (ivt mRNA) coding for antigens. This vaccine format is particularly versatile and allows the production individualised vaccines conferring, T-cell immunity against specific cancer mutations. The CDR3 hypervariable regions immune receptors (T-cell receptor, TCR or B-cell BCR) context T- leukaemia lymphoma are targetable sequences, similar to We evaluated functionality an mRNA-based designed trigger EL4 T-lymphoma...