A5034978739- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Skin and Cellular Biology Research
- Dermatology and Skin Diseases
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Autoimmune Bullous Skin Diseases
- Transgenic Plants and Applications
- Cell Adhesion Molecules Research
- RNA regulation and disease
- Immune Response and Inflammation
- Blood disorders and treatments
- Cancer Immunotherapy and Biomarkers
- Hedgehog Signaling Pathway Studies
- Virus-based gene therapy research
- Skin Protection and Aging
- melanin and skin pigmentation
- vaccines and immunoinformatics approaches
- Dermatological and Skeletal Disorders
- Cellular Mechanics and Interactions
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Erythrocyte Function and Pathophysiology
University of Salzburg
2016-2025
Paracelsus Medical University
2015-2025
EB House Austria
2015-2025
Benaroya Research Institute
2018-2024
Seattle University
2020-2023
University of California, San Francisco
2011-2018
Inserm
2011
University Medical Center Freiburg
2011
Allegheny College
1997
Immune checkpoint blockade is revolutionizing therapy for advanced cancer, but many patients do not respond to treatment. The identification of robust biomarkers that predict clinical response specific inhibitors critical in order stratify and rationally select combinations the context an expanding array therapeutic options.We performed multiparameter flow cytometry on freshly isolated metastatic melanoma samples from 2 cohorts 20 each prior treatment correlated subsequent with tumor immune...
Regulatory T cells (Tregs), which are characterized by expression of the transcription factor Foxp3, a dynamic and heterogeneous population that control immune responses prevent autoimmunity. We recently identified subset Tregs in murine skin with properties typical memory defined this as (mTregs). Due to importance these regulating tissue inflammation mice, we analyzed cell humans found almost all normal had an activated phenotype. Compared mTregs peripheral blood, cutaneous unique surface...
Human blood and lymph contain circulating CD4 + CD103 cutaneous resident memory T cells that can seed distant skin sites. See the related Focus by Carbone Gebhardt .
Immune tolerance is maintained in lymphoid organs (LOs). Despite the presence of complex immune cell networks non-LOs, it unknown whether self-tolerance these tissues. We developed a technique to restrict genetic recombination regulatory T cells (T
Thymic Foxp3-expressing regulatory T cells are activated by peripheral self-antigen to increase their suppressive function, and a fraction of these survive as memory (mTregs). mTregs persist in nonlymphoid tissue after cessation Ag expression have enhanced capacity suppress tissue-specific autoimmunity. In this study, we show that murine express specific effector cell markers localize preferentially hair follicles skin. Memory Tregs high levels both IL-2Rα IL-7Rα. Using genetic-deletion...
Abstract Peripheral immune cell infiltration into the brain is a prominent feature in aging and various neurodegenerative diseases such as Alzheimer’s disease (AD). As AD progresses, CD8+ T cells infiltrate parenchyma, where they tightly associate with neurons microglia. The functional properties of are largely unknown. To gain further insights putative functions brain, we explored compared transcriptomic profile isolated from blood transgenic (APPswe/PSEN1dE9, line 85 [APP-PS1]) age-matched...
Immune homeostasis in peripheral tissues is achieved by maintaining a balance between pathogenic effector T cells (Teffs) and protective Foxp3(+) regulatory (Tregs). Using mouse model of an inducible tissue Ag, we demonstrate that Ag persistence major determinant the relative frequencies Teffs Tregs. Encounter transferred naive CD4(+) with transiently expressed leads to generation cytokine-producing Persistent expression mimic self-antigen, functional inactivation loss preservation Tregs...
Abstract After activation, CD4+ Th cells differentiate into functionally specialized populations that coordinate distinct immune responses and protect against different types of pathogens. In humans, these effector memory cell subsets can be readily identified in peripheral blood based on their differential expression chemokine receptors govern homeostatic inflammatory trafficking. Foxp3+ regulatory T (Treg) also divided phenotypically mirror each share key transcription factors cytokines....
Darier disease (DD) is a rare, inherited multi-organ disorder associated with mutations in the ATP2A2 gene. DD patients often have skin involvement characterized by malodorous, inflamed and recurrent, severe infections. Therapeutic options are limited inadequate for long-term management of this chronic disease. The aim study was to characterize cutaneous immune infiltrate lesions detail identify new therapeutic targets. Using gene protein expression profiling assays including scRNA...
Genetic activation of hedgehog/glioma-associated oncogene homolog (HH/GLI) signaling causes basal cell carcinoma (BCC), a very frequent nonmelanoma skin cancer. Small molecule targeting the essential HH effector Smoothened (SMO) has proven an effective therapy BCC, though development drug resistance poses major challenges to anti-HH treatments. In light recent breakthroughs in cancer immunotherapy, we analyzed possible immunosuppressive mechanisms HH/GLI-induced BCC detail. Using genetic...
Abstract Recessive dystrophic epidermolysis bullosa (RDEB) is caused by mutations in COL7A1 , leading to loss or dysfunction of type‐VII collagen (C7), a protein essential for skin stability. Clinically, patients suffer from severe blistering, chronic recurrent wounds, and scarring, which predispose early onset aggressive squamous cell carcinoma. Previous studies showed that RDEB‐keratinocytes (RDEB‐KC) express high levels matrix‐metalloproteinase 9 (MMP‐9), molecule known play crucial role...
Natural killer (NK) cells can lyse certain tumor as well virally infected without prior sensitization.Animal studies have shown that general anesthesia (GA) alone or GA followed by surgery decreases both basal NK cytotoxicity and enhancement of activity interferon (IFN). The purpose this study was to determine whether similar inhibition occurs in humans. Venous blood drawn 1 h before 20-24 after under isoflurane/N2 O anesthesia. Peripheral mononuclear (PBMC) were assayed for...
Patients suffering from recessive dystrophic epidermolysis bullosa (RDEB), a hereditary blistering disease of epithelia, show susceptibility to develop highly aggressive squamous cell carcinoma (SCC). Tumors metastasize early and are associated with mortality in the 30th-40th years life this patient group. So far, no adequate therapy is available for RDEB SCC. An approach suicide gene therapy, which death-inducing agent introduced cancer cells. However, lack specificity has constrained...
Natural killer (NK) cells can lyse certain tumor as well virally infected without prior sensitization.Animal studies have shown that general anesthesia (GA) alone or GA followed by surgery decreases both basal NK cytotoxicity and enhancement of activity interferon (IFN). The purpose this study was to determine whether similar inhibition occurs in humans. Venous blood drawn 1 h before 20-24 after under isoflurane/N2 O anesthesia. Peripheral mononuclear (PBMC) were assayed for...