A5030485605- Bee Products Chemical Analysis
- Sperm and Testicular Function
- Antioxidant Activity and Oxidative Stress
- Consumer Attitudes and Food Labeling
- Plant Toxicity and Pharmacological Properties
- Polymer Surface Interaction Studies
- Metabolomics and Mass Spectrometry Studies
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Ferroptosis and cancer prognosis
- Ion Transport and Channel Regulation
- Advanced biosensing and bioanalysis techniques
- Metalloenzymes and iron-sulfur proteins
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Reproductive Biology and Fertility
- Molecular Sensors and Ion Detection
- Fermentation and Sensory Analysis
- Enzyme-mediated dye degradation
- Ginseng Biological Effects and Applications
- Ruminant Nutrition and Digestive Physiology
- Dye analysis and toxicity
- Occupational and environmental lung diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
University of Padua
2000-2025
Institute of Biostructure and Bioimaging
2017
Istituto Nazionale Biostrutture e Biosistemi
2014
Ferroptosis is a form of cell death primed by iron and lipid hydroperoxides prevented GPx4. Ferrostatin-1 (fer-1) inhibits ferroptosis much more efficiently than phenolic antioxidants. Previous studies on the antioxidant efficiency fer-1 adopted kinetic tests where diazo compound generates hydroperoxyl radical scavenged antioxidant. However, this reaction, accounting for chain breaking effect, only minimally useful description inhibition ferrous hydroperoxide dependent peroxidation....
Abstract Background Cellular boundaries of microorganisms can be modified by the expression in cell wall specific proteins endowed with relevant properties, improving their functional performance. So far, surface display (SD) technique had been widely employed yeast Saccharomyces cerevisiae , but it was limited to few laboratory strains and never explored sauvage strains, i.e., isolated from natural environment, which are featured higher levels genetic variability, leading peculiar...
Active uniform films of horseradish peroxidase (HRP) have been prepared by covalent binding on Si/SiO2 or glass supports previously activated silanization and succinylation. Labeling fluorescent Electron Spin Resonance (ESR) probes was used to quantify the surface density active groups peroxidase. Atomic Force Microscopy (AFM) imaging characterize morphology. We observed that a non-uniform protein adsorption due physical interactions present when were not for was, in large part, removed...
A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast Italy, by water and selective tangential flow filtration at different porosity. Since comprehensive, non-ambiguous characterization GSE still difficult task, we resorted to using an integrated combination gel permeation chromatography (GPC) electrospray ionization high resolution mass...
Cytopathology is a noninvasive and cost-effective method for detecting cancer cells in pleural effusions (PEs), although many cases, the diagnostic performance hindered by paucity of significant or lack clear morphological criteria. This study presents results an omics approach to improving PE cytology.Metabolic profiling with proton nuclear magnetic resonance (1 H-NMR) was performed 92 PEs (44 malignant cases 8 different cancers 48 benign 7 nonneoplastic conditions). Light's criteria were...
Metabolomic profiling of human malignant effusion remain a field poorly investigated. Proton nuclear magnetic resonance (1H-NMR) spectroscopy is rapid relatively low cost technique, and an optimal biospecimen suitable for metabonomic investigations. With this study we addressed metabolomic ascitic (mAE) from patients with high grade serous ovarian carcinoma (HGSOC), Hepatocellular (HCC), benign AEs (bAEs) reactive peritonitis.Metabolic 1H-NMR was performed on 72 (31 HGSOC, 16 HCC 25 bAE)...
Abstract Huntington’s disease (HD) is a neurodegenerative disorder caused by CAG-repeat expansions in the huntingtin ( HTT ) gene. The resulting mutant (mHTT) protein induces toxicity and cell death via multiple mechanisms no effective therapy available. Here, we employ genome-wide screening pluripotent mouse embryonic stem cells (ESCs) to identify suppressors of mHTT toxicity. Among identified suppressors, linked HD-associated processes, focus on Metal response element binding transcription...
Endothelial dysfunction plays a key role in the development of liver cirrhosis. Among biomarkers endothelial dysfunction, soluble form Vascular Adhesion Protein-1 (sVAP-1) is an unconventional and less known adhesion molecule endowed also with amine oxidase activity. The aim this study was to explore correlate behavior sVAP-1 that vascular cell molecule-1 (sVCAM-1) intercellular (sICAM-1) severity A cross-sectional carried out by enrolling 28 controls, 59 cirrhotic patients without...
Active uniform films of horseradish peroxidase (HRP) have been prepared by covalent binding on Si/SiO2 or glass supports previously activated silanization and succinylation. Labeling fluorescent Electron Spin Resonance (ESR) probes was used to quantify the surface density active groups peroxidase. Atomic Force Microscopy (AFM) imaging characterize morphology. We observed that a non-uniform protein adsorption due physical interactions present when were not for was, in large part, removed...
The aim of this study was to examine, in biochemical detail, the functional role Arg152 residue selenoprotein Glutathione Peroxidase 4 (GPX4), whose mutation His is involved Sedaghatian-type Spondylometaphyseal Dysplasia. Wild-type and mutated recombinant enzymes with selenopcysteine (Sec) at active site, were purified structurally characterized investigate impact R152H on enzymatic function. did not affect peroxidase reaction's catalytic mechanism, kinetic parameters qualitatively similar...