A5088005163- Gallbladder and Bile Duct Disorders
- Drug Transport and Resistance Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- MicroRNA in disease regulation
- Biliary and Gastrointestinal Fistulas
- Liver Disease Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Renal Diseases and Glomerulopathies
- Cancer-related gene regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Retinoids in leukemia and cellular processes
- Blood disorders and treatments
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Lipid metabolism and disorders
- Heat shock proteins research
- Inflammasome and immune disorders
- Pneumonia and Respiratory Infections
- Acute Kidney Injury Research
- HVDC Systems and Fault Protection
- Ion channel regulation and function
- Liver physiology and pathology
- Drug-Induced Hepatotoxicity and Protection
- Liver Disease and Transplantation
Renji Hospital
2017-2025
Shanghai Jiao Tong University
2017-2025
Cholestasis is a common disease with broad aetiologies. The NOD-like receptor protein 3 (NLRP3) pathway has been demonstrated to play an important role in liver injury and fibrosis induced by cholestasis. We previously proved that MCC950, selective NLRP3 inhibitor, alleviates experimental cholestasis bile-duct ligation (BDL) mice. Herein, we investigate the of calcipotriol, potent vitamin D agonist, cholestasis, test its therapeutic efficacy, explore potential protective mechanism. C57BL/6...
Exosomes derived from mesenchymal stem cell (MSC) alleviate kidney damage through autophagy. This study determined whether MSCs relieve renal fibrosis and inhibit autophagy by exosome transfer of miRNA-122a. The gene expression involved in the mTOR signaling pathway was assessed TGF-β1-treated human tubular epithelial cells (HK-2) unilateral ureteral obstruction (UUO) mice before after MSC-derived exosomes miRNA-122a mimic treatment. Small RNA (sRNA) next-generation sequencing also performed...
A dysregulated inflammatory response and inflammation-associated cell death are central features of renal ischemia-reperfusion injury (IRI). PANoptosis, is a recently recognized form programmed characterized by key pyroptosis, apoptosis necroptosis; however, the specific involvement PANoptosis in IRI remains unknown. By using neutrophil extracellular trap (NETs)-depleted Pad4−/− mice, we found that NETs essential for exacerbating tissue IRI. Single-cell RNA sequencing (scRNA-seq) revealed...
Background: Anti-glomerular basement membrane (anti-GBM) nephritis is mediated by autoantibodies and may progress to end-stage renal disease. Although its pathogenesis not completely understood, dendritic cells (DCs) have been reported play an important role in this process. Hypoxia-inducible factor-2α (HIF-2α) has a regulatory effect on DCs under hypoxic conditions, while no research investigated autoimmune nephritis. Methods: Anti-GBM was induced CD11c-specific HIF-2α-deficient WT mice...
Renal ischemia/reperfusion injury (IRI) is the major cause of acute kidney injury. However, mechanisms underlying sudden loss in function and tissue remain to be fully elucidated. Here, we performed RNA sequencing systematically compare transcriptome differences between IR injured kidneys sham kidneys. We observed that mitochondrial dynamics was destructed renal IRI. Expression fusion-associated genes reduced, whereas expression fission-related increased IRI, these findings were further...
Significance Statement Hypoxia is a hallmark of renal ischemia-reperfusion injury (IRI) and serves as an essential regulator innate immune responses during this process, although the mechanisms regulation remain unclear. Here, we showed in murine model that HIF-2 α knockout dendritic cells (DCs) exacerbated IRI through activation natural killer T cells. Mechanistically, deficiency upregulated CD36 expression DCs, leading to cellular lipid accumulation. Pharmacologic inhibition DCs resulted...
Background Renal ischemia-reperfusion injury (IRI) is an inevitable occurrence during kidney transplantation. Mitophagy, ferroptosis, and the associated immune microenvironment (IME) have been shown to play important roles in renal IRI. However, role of mitophagy-associated IME genes IRI remains unclear. In this study, we aimed construct a prediction model prognosis based on genes. Method The specific biological characteristics gene signature were comprehensively analyzed using public...
To evaluate the efficacy and safety of low-dose trimethoprim (TMP)-sulfamethoxazole (SMX) (TMP-SMX) as primary prophylaxis for Pneumocystis jirovecii pneumonia (PJP) in adult recipients kidney transplantation.Three kinds prescriptions were documented, including 20 mg TMP/100 SMX oral daily, every other day, nonprophylaxis. The outcome was incidence PJP first 180 days follow-up after transplantation. secondary outcomes changes renal liver function.Among 1469 recipients, 1066 (72.56%) received...
Clip migration into the common bile duct (CBD) is a rare but well-established phenomenon of laparoscopic biliary surgery. The mechanism and exact incidence clip are both poorly understood. can cause recurrent cholangitis serve as nidus for stone formation. We present case, 54-year-old woman, clip-induced resulting from surgical 12 months after cholecystectomy exploration (LC+LCBDE) with primary closure.
Changes in mechanosensitive ion channels following radiation have seldom been linked to therapeutic sensitivity or specific factors involved antitumor immunity. Here, this study, we found that the mechanical force sensor, Piezo2, was significantly upregulated tumor cells after radiation, and Piezo2 knockout enhanced growth suppression by radiotherapy. Specifically, loss of induced their IL-15 expression via unleashing JAK2/STAT1/IRF-1 axis radiation. This increase activates IL-15Rα on...
Cholangiocarcinoma (CCA) is a type of solid tumor derived from the bile duct epithelium that features universal gemcitabine resistance. Here, we utilized gene-encoded ROS biosensor probe (HyPer3 probe) to sort subpopulations with different redox statuses CCA cells. The isolated HyPer-low subpopulation cells, which exhibited relatively lower cellular levels, higher chemoresistance than HyPer-high cells in vitro and vivo. Mechanistically, increased expression MTHFD1 was found Knocking down...
Abstract The role of RNA N6-methyladenosine (m6A) modification in immunity is being elucidated. This study aimed to explore the potential association between m6A regulators and immune microenvironment IgA nephropathy (IgAN). expression profiles 24 107 IgAN patients were obtained from Gene Expression Omnibus (GEO) database. least absolute shrinkage selection operator (LASSO) regression logistic analysis utilized construct a model for distinguishing control samples. Based on levels regulators,...