A5048834582- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Fungal Infections and Studies
- Bacterial Identification and Susceptibility Testing
- Antifungal resistance and susceptibility
- Escherichia coli research studies
- Infections and bacterial resistance
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Microtubule and mitosis dynamics
- Hepatitis B Virus Studies
- Plant Pathogenic Bacteria Studies
- Protist diversity and phylogeny
- Chemical Reactions and Isotopes
- Streptococcal Infections and Treatments
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Genomics and Rare Diseases
- Amoebic Infections and Treatments
- Advanced biosensing and bioanalysis techniques
National Health Research Institutes
2009-2025
National Defense Medical Center
2013-2020
Tri-Service General Hospital
2013-2020
Tigecycline is regarded as a last-resort treatment for carbapenem-resistant Klebsiella pneumoniae (CRKP) infections, but increasing numbers of tigecycline-resistant K. isolates have been reported. The tigecycline resistance mechanisms in CRKP are undetermined. This study aimed to elucidate the underlying 16 tigecycline- and (TCRKP) isolates. Mutations determinant genes [ramR, acrR, oqxR, tet(A), tet(L), tet(X), tet(M), rpsJ] were assessed by PCR amplicon sequencing, mutations ramR tet(A)...
Resistance to carbapenems has been documented by the production of carbapenemase or loss porins combined with extended-spectrum β-lactamases AmpC β-lactamases. However, no complete comparisons have made regarding contributions each resistance mechanism towards carbapenem resistance. In this study, we genetically engineered mutants Klebsiella pneumoniae individual and mechanisms, then compared in response ertapenem, imipenem, meropenem, doripenem other antibiotics. Among four studied...
Recent advances in whole-genome sequencing (WGS) have increased the accessibility of this tool, offering substantial potential for pathogen surveillance, outbreak response, and diagnostics. However, routine clinical adoption WGS is hindered by factors such as high costs, technical complexity, requirement bioinformatics expertise data analysis. To address these challenges, we propose RapidONT, a workflow designed cost-effective accessible WGS-based RapidONT employs mechanical shearing–based...
Multilocus sequence typing (MLST) is one of the most commonly used methods for studying microbial lineage worldwide. However, traditional MLST process using Sanger sequencing time-consuming and expensive. We have designed a workflow that simultaneously sequenced seven full-length housekeeping genes 96 meticillin-resistant Staphylococcus aureus isolates with dual-barcode multiplexing just single flow cell an Oxford Nanopore Technologies MinION system, then we performed bioinformatic analysis...
The streptomycin counter-selection system is a useful tool for constructing unmarked in-frame gene deletions, which fundamental approach to study bacteria and their pathogenicity at the molecular level. A prerequisite this acquiring streptomycin-resistant strain due rpsL mutations, encodes ribosomal protein S12. However, in no resistance was found be caused by mutations all 127 clinical strains of Klebsiella pneumoniae isolated from liver abscess patients. By screening 107 spontaneous...
Abstract Background Blood cultures are essential for diagnosing bloodstream infections, but current phenotypic tests antimicrobial resistance (AMR) provide limited information. Oxford Nanopore Technologies introduces nanopore sequencing with adaptive sampling, capable of real-time host genome depletion, yet its application directly from blood remains unexplored. This study aimed to identify pathogens and predict AMR using sequencing. Methods In this cross-sectional genomic study, 458...
Molecular typing is an essential tool that has been extensively applied in laboratories as well clinical settings. Next-generation sequencing technologies promise high-throughput and cost-effective molecular applications; however, the accessibility of these limited due to high capital cost. Oxford Nanopore Technologies (ONT) offers a MinION device with advantages real-time data analysis, rapid library preparation, low cost per test. However, are often overshadowed by its lower raw accuracy....
A strategic system for the screening and testing of new antibiotics was created to facilitate development that are robustly effective against MDR bacteria.In-frame deletion, site-directed mutagenesis plasmid transformation were used generate genetically engineered strains with various resistance mechanisms from a fully susceptible clinical isolate Klebsiella pneumoniae. Antimicrobial susceptibility mouse infection model test these in vitro vivo, respectively.A total 193 strains, including 29...
Klebsiella pneumoniae raises significant concerns to the health care industry as these microbes are source of widespread contamination medical equipment, cause pneumonia well other multiorgan metastatic infections and have gained multidrug resistance. Despite soaring mortality rates, host cell alterations occurring during remain poorly understood. Here, we show that in vitro vivo K. lung epithelia, microtubules severed then eliminated. This destruction does not require direct association...
Third-generation cephalosporin-resistant Escherichia coli (CREC), particularly strains producing extended-spectrum β-lactamases (ESBLs), are a global concern. Our study aims to longitudinally assemble the genomic characteristics of CREC isolates from fecal samples an index patient with recurrent CREC-related urinary tract infections and his family swabs home environment 12 times between 2019 2021 investigate distribution antibiotic resistance genes. identified using VITEK 2 were subjected...
Klebsiella pneumoniae is an enteric bacterium known to cause pneumonia, urinary tract infections and pyogenic liver abscesses. Of these infections, patients who develop pneumonia have poor survival rates alarming mortality of up 44% in highly infected individuals. Many studies focused on identifying bacterial components crucial the disease process, but underlying sub‐cellular mechanisms for progression remained elusive. To begin study mechanisms, we assessed cytoskeletal integrity A549 lung...