A5048677608- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Leptospirosis research and findings
- Cancer Immunotherapy and Biomarkers
- Microbial infections and disease research
- Viral Infections and Vectors
- RNA Interference and Gene Delivery
- Reproductive tract infections research
- Advanced Drug Delivery Systems
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- T-cell and B-cell Immunology
- Streptococcal Infections and Treatments
- Viral Infections and Outbreaks Research
- Criminal Justice and Penology
- Nanoparticle-Based Drug Delivery
- Zoonotic diseases and public health
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Virus-based gene therapy research
- Syphilis Diagnosis and Treatment
- Immune cells in cancer
- Social Issues and Policies in Latin America
- Comparative constitutional jurisprudence studies
Universidad de Navarra
2015-2024
Navarre Institute of Health Research
2017-2024
Instituto de Medicina Tropical “Pedro Kourí”
2002-2022
ORCID
2022
Clinica Universidad de Navarra
2022
Ministerio de Salud
2022
Bipar
2021
Fraunhofer Institute for Production Systems and Design Technology
2007
Instituto de Medicina Tropical
2007
Centro Nacional de Sanidad Agropecuaria
1995
Combined PD-1 and CTLA-4-targeted immunotherapy with nivolumab ipilimumab is effective against melanoma, renal cell carcinoma non-small-cell lung cancer1–3. However, this comes at the cost of frequent, serious immune-related adverse events, necessitating a reduction in recommended dose that given to patients4. In mice, co-treatment surrogate anti-PD-1 anti-CTLA-4 monoclonal antibodies transplantable cancer models, but also exacerbates autoimmune colitis. Here we show treating mice clinically...
Poly I:C is a powerful immune adjuvant as result of its agonist activities on TLR-3, MDA5 and RIG-I. BO-112 nanoplexed formulation complexed with polyethylenimine that causes tumor cell apoptosis showing immunogenic death features which upon intratumoral release results in more prominent infiltration by T lymphocytes. Intratumoral treatment subcutaneous tumors derived from MC38, 4 T1 B16-F10 leads to remarkable local disease control dependent type-1 interferon gamma-interferon. Some degree...
Background BO-112 is a nanoplexed form of polyinosinic:polycytidylic acid that acting on toll-like receptor 3 (TLR3), melanoma differentiation-associated protein 5 (MDA5) and kinase RNA-activated (PKR) elicits rejection directly injected transplanted tumors, but has only modest efficacy against distant untreated tumors. Its clinical activity also been documented in early phase trials. The 5,6-dimethylxanthenone-4-acetic (DMXAA) stimulator interferon genes (STING) agonist shows comparable...
Abstract The ability of conventional type-1 dendritic cells (cDC1) to cross-present tumor antigens CD8+ T is critical for the induction antitumor CTLs. Mice that are constitutively deficient in cDC1 have been reported fail respond immunotherapy strategies based on checkpoint inhibitors. However, further work needed clarify precise time during treatment required beneficial effect treatment. Here, we used a refined XCR1-DTR-Venus transgenic mouse model acutely deplete and trace their behavior...
CD137 (4-1BB) costimulation imprints long-term changes that instruct the ultimate behavior of T cells have previously experienced ligation. Epigenetic could provide a suitable mechanism for these consequences. Genome-wide DNA methylation arrays were carried out on human peripheral blood CD8+ lymphocytes stimulated with agonist monoclonal antibody to CD137, including urelumab, which is in phase I/II clinical trials cancer immunotherapy. Several genes showed consistent patterns response...
Article19 November 2019Open Access Source DataTransparent process Repurposing the yellow fever vaccine for intratumoral immunotherapy Maria Angela Aznar Corresponding Author [email protected] orcid.org/0000-0002-7970-1672 Center Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain Search more papers by this author Carmen Molina Alvaro Teijeira CIBERONC, Madrid, Instituto de investigación Navarra (IDISNA), Inmaculada Rodriguez Arantza Azpilikueta Saray Garasa Alfonso R...
Activation-induced cell death (AICD) is a complex immunoregulatory mechanism that causes the demise of fraction T-lymphocytes upon antigen-driven activation.In present study we investigated direct role TNF in AICD CD8 T lymphocytes.Methods: Human peripheral mononuclear cells were isolated from healthy donors and fresh tumor-infiltrating lymphocytes obtained cancer patients undergoing surgery.T activated with anti-CD3/CD28 mAbs or pool virus peptides, combination clinicalgrade blocking...
Abstract NK‐cell reactivity against cancer is conceivably suppressed in the tumor microenvironment by interaction of inhibitory receptor NKG2A with non‐classical MHC‐I molecules HLA‐E humans or Qa‐1 b mice. We found that intratumoral delivery NK cells attains significant therapeutic effects only if co‐injected anti‐NKG2A and anti‐Qa‐1 blocking monoclonal antibodies solid mouse models. Such activity was contingent on endogenous CD8 T type‐1 conventional dendritic (cDC1). Moreover, anti‐tumor...
Journal Article N-Acetylcysteine enhances T cell functions and growth in culture Get access Edward Eylar, Eylar Department of Biochemistry, Ponce School MedicinePonce, Puerto Rico 00732 Correspondence to: E. Eyler Search for other works by this author on: Oxford Academic PubMed Google Scholar Cynthia Rivera-Quinones, Rivera-Quinones Carmen Molina, Molina Ineavely Báez, Báez Félix M. Mercado International Immunology, Volume 5, Issue 1, January 1993, Pages 97–101,...
Objective: To assess early intervention with pimecrolimus combined corticosteroid (CS) for major flares in patients severe atopic dermatitis (AD). Methods: In this 6‐month, double‐blind, multicenter, randomized, vehicle‐controlled, parallel‐group 35 US centers, 275 children aged 3 months to 11 years mild AD applied the study medication twice daily at first signs/symptoms of AD. For not controlled medication, a mid‐potency CS cream replaced evening drug up weeks. The percentage subjects no...
CD69 is an early activation marker on the surface of T lymphocytes undergoing by cognate antigen. We observed intense expression tumor-infiltrating T-lymphocytes that reside in hypoxic tumor microenvironment and hypothesized could be, at least partially, under control transcriptional hypoxia response. In line with this, human mouse CD3-stimulated cultured (1% O2) showed increased protein mRNA level. Consistent these findings, had recently undergone vivo, as denoted pimonidazole staining,...
BO-112 is a poly I:C-based viral mimetic that exerts anti-tumor efficacy when intratumorally delivered in mouse models. Intratumoral synergizes mice with systemic anti-PD-1 mAbs and this combination has attained PD1-refractory melanoma patients. We sought to evaluate the of pre-surgically applied neoadjuvant settings have observed repeated intratumoral injections prior surgical excision primary tumor significantly reduced metastasis from orthotopically implanted 4T1-derived tumors...
In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance these was assessed DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR performed on same before and after formalin-fixed, paraffin-embedding procedure (FFPE tissues). rDNA detected all fresh FFPE positive while rDNA-based protocol...
ABSTRACT. Although infection with Leishmania braziliensis is perhaps the key reason to treat New World cutaneous leishmaniasis (CL) and mucosal (ML), total literature contains relatively few reported cases. With aim of supplementing meager clinical information available, we searched records Jorochito (Dermatology) Hospital, Bolivia, for years 1999–2020 identified treatment 1,696 naive CL patients 355 ML patients. Because follow-up was poor this real-world experience in developing world, only...