A5042272594- Cancer Genomics and Diagnostics
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Genetic factors in colorectal cancer
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Treatments and Studies
- Head and Neck Cancer Studies
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Cancer Treatment and Pharmacology
- Cancer Immunotherapy and Biomarkers
- Angiogenesis and VEGF in Cancer
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Oral Health Pathology and Treatment
- Estrogen and related hormone effects
- Advanced Breast Cancer Therapies
- Radiomics and Machine Learning in Medical Imaging
- Immune cells in cancer
- Cancer Diagnosis and Treatment
- Circular RNAs in diseases
- Bladder and Urothelial Cancer Treatments
- Cancer-related molecular mechanisms research
- Inflammatory mediators and NSAID effects
- Lymphoma Diagnosis and Treatment
Instituto de Investigación Biomédica de Málaga
2016-2024
Centro de Investigación Biomédica en Red de Cáncer
2022-2024
Roche (Spain)
2022-2024
Universidad de Málaga
2015-2024
Hospital Clínico Universitario Virgen de la Victoria
2005-2023
Hospital Universitario de Valme
2023
Fundació Clínic per a la Recerca Biomèdica
2023
Andalusian Centre for Nanomedicine and Biotechnology
2022
Hospital Costa del Sol
2016
Complejo Hospitalario de Jaén
2016
Combined PD-1 and CTLA-4-targeted immunotherapy with nivolumab ipilimumab is effective against melanoma, renal cell carcinoma non-small-cell lung cancer1–3. However, this comes at the cost of frequent, serious immune-related adverse events, necessitating a reduction in recommended dose that given to patients4. In mice, co-treatment surrogate anti-PD-1 anti-CTLA-4 monoclonal antibodies transplantable cancer models, but also exacerbates autoimmune colitis. Here we show treating mice clinically...
Triple negative breast cancer (TNBC) is a heterogeneous disease with distinct molecular subtypes that differentially respond to chemotherapy and targeted agents. The purpose of this study explore the clinical relevance Lehmann TNBC by identifying any differences in response neoadjuvant among them. We determined gene expression profiling paraffined pre-treatment tumor biopsies from 125 patients treated anthracyclines and/or taxanes +/- carboplatin. explored clinicopathological characteristics...
Recurrence risk in breast cancer varies throughout the follow-up time. We examined if these changes are related to level of expression proliferation pathway and intrinsic subtypes.
Most hormone receptor (HR)(+)/HER2(-) breast cancer patients respond unfavorably to neoadjuvant chemotherapy (NAC); however, genomic tests may identify those who are likely benefit. Using the Prosigna assay, we first evaluated technical performance of core needle biopsy (CNB) tissues. We then determined whether risk relapse (ROR) score and intrinsic subtype predicted response NAC in HR(+)/HER2(-) using CNB samples.Using NanoString's nCounter Dx analysis system a development tissue sample...
Fanconi anemia (FA) patients display an exacerbated risk of oral squamous cell carcinoma (OSCC) and potentially malignant lesions (OPMLs) at early ages. As have defects in their DNA repair mechanisms, standard-of-care treatments for OSCC such as radiotherapy chemotherapy, give rise to severe toxicities. New methods diagnosis are urgently needed allow treatment disease stages achieve better clinical outcomes. We conducted a prospective, longitudinal study wherein liquid biopsies from sixteen...
Circulating tumor DNA (ctDNA) has emerged as a non-invasive “liquid biopsy” for early breast cancer diagnosis. We evaluated the suitability of ctDNA analysis in diagnosis after mammography findings, comparing PIK3CA and TP53 mutations between biopsies pre-biopsy circulating DNA. Matched plasma frozen fresh tissue from patients with Breast Imaging-Reporting Data System (BIRADS) 4c/5 findings subsequent primary were analyzed using NGS TruSeq Custom Amplicon Low Input Panel (Illumina) SafeSEQ...
Abstract KRAS mutations are common in colorectal cancer (CRC). In this setting, mutation status determination circulating-free DNA from blood samples (liquid biopsy) has been shown to be a viable alternative tissue testing. The objective of study was compare the sensitivity two liquid biopsy methods for detecting plasma metastatic CRC patients. Samples with positive (KRAS-MUT+) result and mutant allelic fraction (MAF) < 5% according OncoBEAM RAS assay were pairly analyzed by Idylla ctKRAS...
Breast cancer (BC) continues to pose a significant burden on global cancer-related morbidity and mortality, primarily driven by metastasis. However, the combined influence of microRNAs (miRNAs) intratumoral microbiota BC metastasis remains largely unexplored. In this study, we aimed elucidate interplay between composition, miRNA expression profiles, their collective development in patients employing 16S rRNA sequencing qPCR methodologies. Our findings revealed an increase miR-149-5p,...
•Complete genotyping in mCRC is essential the selection of optimal therapy first line.•Adoption ctDNA testing into clinical practice has potential to improve care patients with mCRC.•Testing can identify biomarkers a significantly shorter TAT than SOC tissue.•Use validated, high-sensitivity comprehensive allows fully informed treatment decisions be made earlier.•Implementing ahead tissue time initiation without compromising biomarker discovery. BackgroundComprehensive selecting for...
Human papillomavirus (HPV)-related head and neck cancer has been associated with an improved prognosis in patients treated radiotherapy (RT) +/− chemotherapy (CT); however, RT combined epidermal growth factor receptor (EGFR) inhibitors not fully studied this group of patients. Immunohistochemical expression p16 PCR HPV16 DNA were retrospectively analyzed tumor blocks from 108 stage III/IV RT+CT (56) or RT+EGFR (52). Disease-free survival (DFS) overall (OS) by the Kaplan-Meier method. was...
Analysis of circulating tumor DNA (ctDNA) is increasingly used for clinical decision-making in oncology. However, ctDNA could represent ≤ 0.1 % cell-free early-stage tumors and its detection requires high-sensitive techniques such as digital PCR (dPCR).
In the present paper we describe facts that took place in rat brain after a single injection of enzyme neuraminidase from Clostridium perfringens into right lateral ventricle. After injection, it diffused through cerebrospinal fluid ipsilateral ventricle and third ventricle, about 400 µm periventricular parenchyma. The expression ICAM1 endothelial cells vessels, IBA1 microglia, GFAP astrocytes notably increased regions reached by injected neuraminidase. subependymal microglia ventricular...
Resistance to Immune Checkpoint Blockade (ICB) constitutes the current limiting factor for optimal implementation of this novel therapy, which otherwise demonstrates durable responses with acceptable toxicity scores. This limitation is exacerbated by a lack robust biomarkers. In study, we have dissected basal TME composition at gene expression and cellular levels that predict response Nivolumab prognosis. BCR, TCR HLA profiling were employed further characterization molecular variables...
Fanconi anemia (FA) patients frequently develop oral squamous cell carcinoma (OSCC). This cancer in FA is diagnosed within the first 3-4 decades of life, very often preceded by lesions that suffer a malignant transformation. In addition, they respond poorly to current treatments due toxicity or multiple recurrences. Translational research on new chemopreventive agents and therapeutic strategies has been unsuccessful partly scarcity disease models failure fully reproduce disease. Here we...
Fanconi anemia (FA) patients display an exacerbated risk of oral squamous cell carcinoma (OSCC) and precursor lesions at young ages, mainly the cavity. As have defects in DNA repair mechanisms, standard-of-care treatments to OSCC such as radiotherapy chemotherapy give rise severe toxicities. New methods for early diagnosis are urgently necessary allow disease stages achieve better clinical outcomes. We conducted a prospective, longitudinal study whereby liquid biopsies from sixteen...
Abstract Background To investigate the incidence of non-cancer mortalities and prognostic factors associated with competitive causes death in a homogeneous cohort patients locally advanced head neck cancer treated radiotherapy systemic treatment. Methods This study included 284 treatment between 2005 2017. The cumulative tumour, second tumours, treatment, side effects comorbidity was calculated. A Fine Gray regression model used to mortality. Results tumoral at 5 10 years were 35 47%...
Early breast cancer patients often experience relapse due to residual disease after treatment. Liquid biopsy is a methodology capable of detecting tumor components in blood, but low concentrations at early stages pose challenges. To detect them, next-generation sequencing has promise entails complex processes. Exploring larger blood volumes could overcome detection limitations. Herein, total 282 high-volume plasma and blood-cell samples were collected for dual ctDNA/CTCs using single...
Background: Double blockade with pertuzumab and trastuzumab combined chemotherapy is the standard neoadjuvant treatment for HER2-positive early breast cancer. Data derived from clinical trials indicates that response rates differ among intrinsic subtypes of The aim this study to determine if these results are valid in real-world patients. Methods: A total 259 patients treated eight Spanish hospitals were included divided into two cohorts: Cohort (132 patients) received plus (NAC), B NAC (122...
We performed three different studies to obtain additional data on the biological and prognostic role of vascular endothelial growth factor C (VEGFC) in classical Hodgkin lymphoma (cHL): (1) serum VEGFC levels were measured by enzyme-linked immunosorbent assay 80 patients; (2) immunohistochemical expression tumor tissue was evaluated using a case-control study 62 (3) gene investigated vitro six cHL cell lines, compared with inflammatory lymph nodes. Higher found patients than healthy...
Objetive: Angiogenesis is stimulated by angiogenic factors released tumour cells, though other such as tumour-associated macrophages (TAMs), also contribute towards increasing the process in colorectal cancer (CRC). The aim of th
Abstract Background Vascular endothelial growth factor (VEGF) expression seems to be associated with worse overall survival in patients head and neck squamous cell carcinoma. The purposes of this study were assess the prognostic values immunohistochemical VEGF vascular receptor‐2 (VEGFR‐2) a cohort operable advanced laryngeal cancer who had been treated induction chemotherapy. Methods VEGFR‐2 malignant cells was quantified by an enzyme immunosorbent assay retrospective series. Results...