A5064876298- Drug-Induced Adverse Reactions
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Urticaria and Related Conditions
- Immunotherapy and Immune Responses
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Contact Dermatitis and Allergies
- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Mast cells and histamine
- Cancer-related gene regulation
- Vascular Anomalies and Treatments
- Extracellular vesicles in disease
- Polyamine Metabolism and Applications
- Chemotherapy-induced cardiotoxicity and mitigation
- Algal biology and biofuel production
- Cardiac Fibrosis and Remodeling
- Gastric Cancer Management and Outcomes
- Cardiac Ischemia and Reperfusion
- Hematopoietic Stem Cell Transplantation
Imperial College London
2017-2025
Lung Institute
2023-2024
Instituto de Investigación Biomédica de Málaga
2021-2023
NIHR Imperial Biomedical Research Centre
2023
Andalusian Centre for Nanomedicine and Biotechnology
2022-2023
Universidad de Málaga
2007-2022
Universidad de Navarra
2022
Hospital Clínico Universitario Virgen de la Victoria
2021
British Heart Foundation
2018-2020
Lund University
2011-2019
For monogenic diseases caused by pathogenic loss-of-function DNA variants, attention focuses on dysregulated gene-specific pathways, usually considering molecular subtypes together within causal genes. To better understand phenotypic variability in hereditary hemorrhagic telangiectasia (HHT), we subcategorized variants ENG/endoglin, ACVRL1/ALK1, and SMAD4 if they generated premature termination codons (PTCs) subject to nonsense-mediated decay. In 3 patient cohorts, a PTC-based classification...
O Transtorno do Espectro Autista (TEA) é caracterizado por dificuldades na interação social, comunicação e comportamentos repetitivos. A terapia ocupacional fundamental para apoiar o desenvolvimento de indivíduos, focando promoção da independência participação em atividades diárias. abordagem integração sensorial ajuda a regular as respostas sensoriais, permitindo melhor adaptação ao ambiente maior autonomia. avaliação não padronizada combina observações clínicas com escalas padronizadas,...
The most common cause of death due to COVID-19 remains respiratory failure. Yet, our understanding the precise cellular and molecular changes underlying lung alveolar damage is limited. Here, we integrate single cell transcriptomic data donor tissue with spatial stratifying histopathological stages diffuse damage. We identify in composition across progressive damage, including waves molecularly distinct macrophages depletion epithelial endothelial populations. Predicted markers pathological...
Summary Non-immediate reactions to iodine contrast media (ICM) affect 2–5% of patients receiving these agents. We studied the immunological mechanisms involved in with a confirmed non-immediate reaction, maculopapular exanthema, after administration ICM. The diagnosis was carried out by skin testing or drug provocation test. study performed sequential peripheral blood mononuclear cells taken from onset reaction flow cytometry and biopsy immunohistochemistry, specific recognition lymphocyte...
Background: Maculopapular exanthema (MPE) is the most frequent clinical manifestation of nonimmediate allergic reactions to drugs and T helper 1 (Th1) cytokines CD4 + cells have been shown play an important role in its pathogenesis. We assessed chemokines their receptors pathogenesis MPE. Methods: evaluated skin biopsies peripheral CD8 from 27 patients during acute phase reaction 26 exposed controls. Semiquantitative real‐time PCR was performed determine expression immunohistochemistry used...
Mesenchymal stromal cells (MSCs) are currently being evaluated in numerous pre-clinical and clinical cell-based therapy studies. Furthermore, there is an increasing interest exploring alternative uses of these disease modelling, pharmaceutical screening, regenerative medicine by applying reprogramming technologies. However, the limited availability MSCs from various sources restricts their use. Term amniotic fluid has been proposed as source MSCs. Previously, only low volumes term its...
The functions of retinoic acid (RA), a potent morphogen with crucial roles in embryogenesis including developmental hematopoiesis, have not been thoroughly investigated the human setting. Using an vitro model hematopoietic development, we evaluated effects RA signaling on development blood and generated progenitors. Decreased increases generation cells stem cell (HSC)-like phenotype, capable differentiation into myeloid lymphoid lineages, through two separate mechanisms: by increasing...
To cite this article: Chaves P, Torres MJ, Aranda A, Lopez S, Canto G, Blanca M, Mayorga C. Natural killer–dendritic cell interaction in lymphocyte responses hypersensitivity reactions to betalactams. Allergy 2010; 65 : 1600–1608. Abstract Background: Betalactams are the commonest cause of allergic mediated by either IgE or T lymphocytes which innate and adaptive immune systems mediate earlier stages immunological responses. One links between these is related natural killer (NK) dendritic...
Given the poor track record to date of animal models for creating cardioprotective drugs, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have been proposed as a therapeutically relevant platform guide target validation and cardiac drug development. Mitogen-Activated Protein Kinase Kinase-4 (MAP4K4) is an "upstream" member MAPK superfamily that implicated in muscle cell death from oxidative stress, based on gene silencing pharmacological inhibition hPSC-CMs. A further role...
Resistance to Immune Checkpoint Blockade (ICB) constitutes the current limiting factor for optimal implementation of this novel therapy, which otherwise demonstrates durable responses with acceptable toxicity scores. This limitation is exacerbated by a lack robust biomarkers. In study, we have dissected basal TME composition at gene expression and cellular levels that predict response Nivolumab prognosis. BCR, TCR HLA profiling were employed further characterization molecular variables...
Abstract Within the hematopoietic system, Notch pathway is critical for promoting thymic T cell development and suppressing B myeloid lineage fates; however, its impact on NK lymphopoiesis less understood. To study role of during in vivo, we investigated different compartments function Rbp-Jkfl/flVav-Cretg/+ mice, which Rbp-Jk, major transcriptional effector canonical signaling, was specifically deleted all cells. Peripheral conventional cytotoxic cells Rbp-Jk–deleted mice were significantly...
Abstract Maf transcription factors are critical regulators of beta-cell function. We have previously shown that reduced MafA expression in human and mouse islets is associated with a pro-inflammatory gene signature. Here, we investigate if the loss induced autoimmune processes pancreas. Transcriptomics analysis showed as well immune cell marker genes. However, clusters CD4+ T B220+ B cells were primarily adult −/− MafB +/− , but not islets. was detected thymus, lymph nodes bone marrow...
Ischemic heart disease remains the foremost cause of death globally, with survivors at risk for subsequent failure. Paradoxically, cell therapies to offset cardiomyocyte loss after ischemic injury improve long-term cardiac function despite a lack durable engraftment. An evolving consensus, inferred preponderantly from non-human models, is that transplanted cells benefit via early paracrine signals. Here, we tested impact signals on human cardiomyocytes, using pluripotent stem cell-derived...
Evidence is growing in favour of a relationship between cancer and chronic inflammation, particularly the role polyamine histamine metabolic interplay involved these physiopathological problems, which are indeed highly complex biological systems. Decodification inter- intra-cellular signalling mechanisms that control effects not an easy task, must be helped by systems biology technologies, including new tools for location integration database-stored information predictive mathematical...