A5030107118- Cancer-related Molecular Pathways
- Fungal and yeast genetics research
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- Bioinformatics and Genomic Networks
- Antifungal resistance and susceptibility
- Protein Kinase Regulation and GTPase Signaling
- Polyamine Metabolism and Applications
- Genetic Neurodegenerative Diseases
- Advanced Breast Cancer Therapies
- RNA Research and Splicing
- Synthesis and biological activity
- Synthesis and pharmacology of benzodiazepine derivatives
- 14-3-3 protein interactions
- ATP Synthase and ATPases Research
- Biological Stains and Phytochemicals
- Synthesis of Organic Compounds
- Sphingolipid Metabolism and Signaling
- Plant biochemistry and biosynthesis
- Mental Health and Psychiatry
- Mental Health Treatment and Access
- PI3K/AKT/mTOR signaling in cancer
- Mitochondrial Function and Pathology
- Cancer Immunotherapy and Biomarkers
Universidade do Porto
2012-2023
Rede de Química e Tecnologia
2012-2023
National Institute of Health Dr. Ricardo Jorge
2020-2022
University of Lisbon
2020-2022
International Olive Council
2019
Abstract Protein kinase C (PKC) isozymes play major roles in human diseases, including cancer. Yet, the poor understanding of isozymes-specific functions and limited availability selective pharmacological modulators PKC have clinical translation PKC-targeting agents. Here, we report first small-molecule PKCδ-selective activator, 7 α -acetoxy-6 β -benzoyloxy-12- O -benzoylroyleanone (Roy-Bz), which binds to PKCδ-C1-domain. Roy-Bz potently inhibited proliferation colon cancer cells by inducing...
// Joana Soares 1, 2 , Liliana Raimundo Nuno A.L. Pereira 4 Ângelo Monteiro Sara Gomes Cláudia Bessa Clara Glória Queiroz 3 Alessandra Bisio 5 João Fernandes 6 Célia Flávio Reis Jorge Gonçalves Alberto Inga Maria M.M. Santos Lucília Saraiva 1 UCIBIO/REQUIMTE, Universidade do Porto, Portugal Laboratório de Microbiologia, Departamento Ciências Biológicas, Faculdade Farmácia, Farmacologia, Medicamento, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Lisboa, CIBIO, Centre...
An inflammatory microenvironment is a tumour-promoting condition that provides survival signals to which cancer cells respond with gene expression changes. One example the alternative splicing variant Rat Sarcoma Viral Oncogene Homolog (Ras)-Related C3 Botulinum Toxin Substrate 1 (RAC1)B, we previously identified in subset of V-Raf Murine B (BRAF)-mutated colorectal tumours. RAC1B was also increased samples from bowel disease patients or an acute colitis mouse model. Here, used...
Yeast has proven to be an efficient model system for functional and pharmacological studies of the p53 tumour suppressor protein. In this work, human p53-MDMX regulatory pathway was reconstituted in yeast. Additionally, by using known inhibitor interaction, SJ-172550, efficacy a simplified yeast-based screening assay search inhibitors interaction is demonstrated first time. Moreover, further insights on transcriptional activity yeast are provided. particular, it shown that reported wild-type...
Yeast revealed to be a versatile organism for studying endocytosis. Here, inhibition of endocytosis by H(2)O(2) and its correlation with apoptotic cell death were ascertained in Saccharomyces cerevisiae. We found that causes alterations vacuolar morphology concentration-dependent also H(2)O(2)-induced is reversible process occurs the early phase cascade, preceding chromatin condensation DNA fragmentation. Additionally, mutants affecting steps endocytic pathway display sensitivity H(2)O(2)....
The Plectranthus genus is commonly used in traditional medicine due to its potential treat several illnesses, including bacterial infections and cancer. As such, aiming screen the antibacterial cytotoxic activities of extracts, sixteen selected species with medicinal were studied. In total, 31 extracts obtained from 16 spp. tested for their anticancer properties. Well diffusion method was preliminary screening. minimum inhibitory concentration (MIC) minimal bactericidal (MBC) values five...
Aspergillus fumigatus conidia have been linked to severe aspergillosis in immunocompromised patients. Recently, the cytotoxic effect of secondary metabolites from A. was reported. In present work, a methodology used detect cell death markers fungal hyphae adapted study death. Additionally, mechanism H2O2-induced studied for first time. Data presented this work reveal that conidial associated with marked increase TUNEL- and PI-positive cells. It is therefore suggested occurs dose-dependent...
Metabolic reprogramming is a central hub in tumor development and progression. Therefore, several efforts have been developed to find improved therapeutic approaches targeting cancer cell metabolism. Recently, we identified the 7α-acetoxy-6β-benzoyloxy-12-O-benzoylroyleanone (Roy-Bz) as PKCδ-selective activator with potent anti-proliferative activity colon by stimulating PKCδ-dependent mitochondrial apoptotic pathway. Herein, investigated whether antitumor of Roy-Bz, cancer, could be related...
The regulation of protein kinase C (PKC) isoforms by ceramide is still controversial. In this work, the yeast Saccharomyces cerevisiae was used as a model to elucidate effect on activity mammalian PKC isoforms. For that, isc1Δ cells, with deletion in pathway for production hydrolysis complex sphingolipids, individually expressing PKCα, δ and ζ were used. Contrary PKCα ζ, expression PKCδ cells exhibited similar phenotype that observed wild-type treated activator, phorbol 12-myristate...
Introduction:Cancer is an important cause of worldwide mortality, killing more than 8 million people every year.The ovarian cancer one seven types most affect womem, and considered the fatal among gynecologic tumors.The tumor detection presents importants limitations highlighted as difficult gynecological to be diagosed, hidering efficiency treatment consequently patients' survival.Specific molecules produced by tumors could used biomarkers are good targets for diagnosis.Thus, use aptamers...
Background and aimsTo determine the prevalence associated variables with polyvictimization in high school students at Santa Marta, Colombia.Violence is a social concern that affects global public mental health.Violence has an effect on general well-being of communities.In Colombia, it highly probable polyvictimization, person had been subjected to several forms violence.The important for adolescent population because short-and long-term consequences health. Materials methods Results...