A5055560182- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Autism Spectrum Disorder Research
- Anesthesia and Neurotoxicity Research
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- RNA Research and Splicing
- Stress Responses and Cortisol
- Nuclear Structure and Function
- Acute Lymphoblastic Leukemia research
- Genomics and Chromatin Dynamics
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- RNA regulation and disease
- RNA modifications and cancer
- Birth, Development, and Health
- Nanoplatforms for cancer theranostics
- Cognitive Abilities and Testing
- Neuroinflammation and Neurodegeneration Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Photochromic and Fluorescence Chemistry
The University of Texas at Dallas
2013-2024
Pennsylvania State University
2023
Penn State Milton S. Hershey Medical Center
2022
Yale University
2006-2009
Icahn School of Medicine at Mount Sinai
2002-2004
Roswell Park Comprehensive Cancer Center
1999-2001
Cancer Genetics (United States)
1999-2001
The activity-regulated cytoskeletal-associated protein (Arc/Arg3.1) is an immediate early gene that has been widely implicated in hippocampal-dependent learning and memory believed to play integral role synapse-specific plasticity. Here, we examined the of Arc/Arg3.1 amygdala-dependent Pavlovian fear conditioning. We first regulation mRNA after conditioning LTP-inducing stimulation thalamic inputs lateral amygdala (LA). Quantitative real-time PCR, situ hybridization, Western blotting...
Recent studies have shown that nitric oxide (NO) signaling plays a crucial role in memory consolidation of Pavlovian fear conditioning and synaptic plasticity the lateral amygdala (LA). In present experiments, we examined cGMP-dependent protein kinase (PKG), downstream effector NO, long-term potentiation (LTP) at thalamic cortical input pathways to LA. behavioral rats given intra-LA infusions either PKG inhibitor Rp-8-Br-PET-cGMPS or activator 8-Br-cGMP exhibited dose-dependent impairments...
The Neuronal PAS domain protein 4 (Npas4) is a neuronal activity-dependent immediate early gene that has recently been identified as transcription factor which regulates the of genes control inhibitory synapse development and synaptic plasticity. role Npas4 in learning memory, however, currently unknown. Here, we systematically examine auditory Pavlovian fear conditioning, an amygdala-dependent form emotional learning. In our first series experiments, show mRNA are regulated rat lateral...
Reconsolidation updating is a form of memory modification in which an existing can become destabilized upon retrieval and subsequently be modified via protein-synthesis-dependent reconsolidation. However, not all memories appear to destabilize thus are modifiable reconsolidation approaches the neurobiological basis for this remains poorly understood. Here, we report that auditory fear created with 10 tone-shock pairings resistant retrieval-dependent destabilization associated increase...
The RNA-guided Cas9 nuclease, from the type II prokaryotic Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) adaptive immune system has been adapted by scientists to enable site specific genome editing of eukaryotic cells both in vitro and vivo. Previously, we reported development an Adeno-Associated Virus (AAV) mediated CRISPR S. pyogenes (Sp) system, which function can be regulated controlling expression guide RNA (sgRNA) a doxycycline (Dox) dependent manner. Here, report...
The RNA-guided Cas9 nuclease, from the type II prokaryotic Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) adaptive immune system, has been adapted and utilized by scientists to edit genomes of eukaryotic cells. Here, we report development a viral mediated CRISPR/Cas9 system that can be rendered inducible utilizing doxycycline (Dox) delivered cells in vitro vivo adeno-associated virus (AAV). Specifically, developed an gRNA (gRNAi) AAV vector is designed express H1/TO...
Autism Spectrum Disorders (ASD) are complex neurodevelopmental disorders characterized by repetitive behavior and impaired social communication interactions. Apart from these core symptoms, a significant number of ASD individuals display higher levels anxiety some exhibit emotional learning. We therefore sought to further examine learning in an environmentally induced animal model that utilizes the administration known teratogen, valproic acid (VPA) during gestation. Specifically we exposed...
Abstract Remote and minimally‐invasive modulation of biological systems with light has transformed modern biology neuroscience. However, absorption scattering significantly prevents penetration to deep brain regions. Herein, we describe the use gold‐coated mechanoresponsive nanovesicles, which consist liposomes made from artificial phospholipid Rad‐PC‐Rad as a tool for delivery bioactive molecules into tissue. Near‐infrared picosecond laser pulses activated gold‐coating on surface creating...
Abstract Electroconvulsive shock (ECS) is the most effective treatment for depression, but mechanism underlying therapeutic action of this still unknown. To better understand molecular changes that may be necessary clinical effectiveness ECS we have combined technologies gene expression profiling using cDNA microarrays with T7‐based RNA amplification and laser microdissection to identify regulated genes in dentate gyrus granule cell layer hippocampus. We identified previously reported...
In recent years, there has been an increased interest in using recombinant adeno-associated viruses (AAV) to make localized genetic manipulations within the rodent brain. Differing serotypes of AAV possess divergent capsid protein sequences and these variations greatly influence each serotype's ability transduce particular cell types brain regions. We therefore aimed determine serotype that is optimal for targeting neurons Basal Lateral Amygdala (BLA) since transduction efficiency not...
Autism spectrum disorders (ASDs) represent a heterogeneous group of with wide range behavioral impairments including social and communication deficits. Apart from these core symptoms, significant number ASD individuals display higher levels anxiety, some studies indicate that subset have reduced ability to be fear conditioned. Deciphering the molecular basis has been considerably challenging it currently remains poorly understood. In this study we examined autism-like in an environmentally...
Most recent studies aimed at defining the cellular and molecular mechanisms of Pavlovian fear conditioning have focused on protein kinase signaling pathways transcription factor cAMP-response element binding (CREB) that promote memory consolidation in lateral nucleus amygdala (LA). Despite this progress, there still remains a paucity information regarding genes downstream CREB are required for long-term formation LA. We adopted strategy using microarray technology to initially identify...
Existing memories, when retrieved under certain circumstances, can undergo modification through the protein synthesis-dependent process of reconsolidation. Disruption this lead to weakening a memory trace, an approach which is being examined as potential treatment for disorders characterized by pathological such Post-Traumatic Stress Disorder. The success relies upon ability robustly attenuate reconsolidation; however, available literature brings into question reliability various drugs used...
We report that the paired homeodomain transcription factor Pax6 is imported into nucleus by Karyopherin beta family member 13 (Kap13). was identified as a potential cargo for Kap13 yeast two-hybrid screen. Direct binding of to subsequently confirmed in vitro assays with recombinant proteins, and vivo shown coimmunoprecipitation. Ran-dependent import occur using digitonin-permeabilized cells assay. binds via nuclear localization sequence (NLS), which located within segment 80 amino acid...
Viral vectors are frequently used to deliver and direct expression of transgenes in a spatially temporally restricted manner within the nervous system numerous model organisms. Despite common use viral ectopic system, creating high titer that capable expressing very large or difficult express imposes unique challenges. Here we describe development adeno-associated viruses (AAV) lentiviruses designed GluN2A GluN2B subunits N-methyl-D-aspartate receptor (NMDA) receptor, specifically neurons.We...
Transport of macromolecules between the cell nucleus and cytoplasm occurs through nuclear pores is mediated by soluble carriers known as karyopherins (Kaps), transportins, importins, or exportins. We report that Kap β2B (transportin-2) forms complexes with mRNA export factor TAP in presence RanGTP, shown coimmunoprecipitation from HeLa cells. The interaction strictly depends on RanGTP. In digitonin-permeabilized cells, mediates TAP-GFP nuclei A mutant does not coimmunoprecipitate also...
Vagus nerve stimulation (VNS) is a therapeutic intervention previously shown to enhance fear extinction in rats. VNS approved for use humans the treatment of epilepsy, depression, and stroke, it currently under investigation as an adjuvant exposure therapy PTSD. However, mechanisms by which enhances conditioned remain unresolved. increases norepinephrine levels extinction-related pathways, but recent studies indicate that release from locus coeruleus interferes with learning. The purpose...