A5077669075- Colorectal Cancer Treatments and Studies
- Colorectal Cancer Screening and Detection
- Genetic factors in colorectal cancer
- Prostate Cancer Treatment and Research
- Cancer Immunotherapy and Biomarkers
- Nutritional Studies and Diet
- Cancer, Lipids, and Metabolism
- Prostate Cancer Diagnosis and Treatment
- Nutrition and Health in Aging
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Folate and B Vitamins Research
- Immune Cell Function and Interaction
- Genetic Associations and Epidemiology
- Estrogen and related hormone effects
- Nutrition, Genetics, and Disease
- Colorectal Cancer Surgical Treatments
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Lung Cancer Treatments and Mutations
- Cancer-related Molecular Pathways
- Genomic variations and chromosomal abnormalities
- Genomics and Rare Diseases
Mayo Clinic
2012-2025
WinnMed
2014-2023
Mayo Clinic in Arizona
2009-2022
University of Iowa
2011-2013
Fred Hutch Cancer Center
2012-2013
University of Minnesota
2011-2013
Huntsman Cancer Institute
2013
University of Utah
2013
University of Southern California
2013
Keck Hospital of USC
2012
Cigarette smoking is an established risk factor for colorectal cancer. Because carcinogenesis a heterogeneous process, we investigated whether cigarette differentially associated with molecularly defined subtypes of cancer.We evaluated associations between and incident cancer, overall by microsatellite instability (MSI) phenotype (MSI-high vs MSI-low or stable), CpG island methylator (CIMP positive CIMP negative), BRAF mutation status (BRAF among 37 399 participants in population-based...
Tumor budding in colorectal carcinoma has been associated with poor outcome multiple studies, but the absence of an established histologic cutoff for "high" tumor budding, heterogeneity study populations, and varying methods assessing have hindered widespread incorporation this parameter clinical reports. We used scoring system a population-based cohort to determine confirm its prognostic significance. retrieved hematoxylin eosin–stained sections from 553 incident cases. Each case was...
The role of the innate immune response in colorectal cancer is understudied. We examined survival patients relation to eosinophils, cells, infiltrating tumor. Tissue microarrays were constructed from paraffin-embedded tumor tissues collected between 1986 and 2002 441 post-menopausal women diagnosed with Iowa Women's Health Study. stained an eosinophil peroxidase antibody. Eosinophils epithelial stromal within (called hereafter) counted scored into three four categories, respectively. In...
Functional characterization of disease-causing variants at risk loci has been a significant challenge. Here we report high-throughput single-nucleotide polymorphisms sequencing (SNPs-seq) technology to simultaneously screen hundreds thousands SNPs for their allele-dependent protein-binding differences. This takes advantage higher retention rate protein-bound DNA oligos in protein purification column quantitatively sequence these SNP-containing oligos. We apply this test prostate cancer-risk...
Multiple studies have identified loci associated with the risk of developing prostate cancer but genes are not well studied. Here we create a normal tissue-specific eQTL data set and apply this to previously (PrCa)-risk SNPs in an effort identify candidate target genes. The is constructed by genotyping RNA sequencing 471 samples. We focus on 146 PrCa-risk SNPs, including all linkage disequilibrium each SNP, resulting 100 unique intervals. analyse cis-acting associations where transcript...
Abstract Functional annotations have been shown to improve both the discovery power and fine-mapping accuracy in genome-wide association studies. However, optimal strategy incorporate large number of existing is still not clear. In this study, we propose a Bayesian framework functional systematic manner. We compute maximum posteriori solution use cross validation find penalty parameters. By extending our previous method CAVIARBF into framework, require only summary statistics as input. also...
Purpose In men who are at high-risk of prostate cancer, progression and death from cancer after radical retropubic prostatectomy (RRP), limited prognostic information is provided by established features. The objective this study was to develop a model predictive outcome in group patients. Methods Candidate genes were identified microarray expression data 102 laser capture microdissected tissue samples. Candidates overexpressed tumor compared with normal more frequently Gleason patterns 4 5...
Chromosome 8q24 locus contains regulatory variants that modulate genetic risk to various cancers including prostate cancer (PC). However, the biological mechanism underlying this regulation is not well understood. Here, we developed a chromosome conformation capture (3C)-based multi-target sequencing technology and systematically examined three PC regions at their potential targets across human genome in six cell lines. We observed frequent physical contacts of with multiple genomic regions,...
Host immune response may predict the course of colorectal cancer. We examined survival 468 cancer patients associated with two tumor-infiltrating biomarkers, number cytotoxic T lymphocytes (CTLs), and activated CTLs, as reflected by cells expressing granzyme B (GZMB) in prospective Iowa Women's Health Study.
Abstract Purpose: This paper describes a process for the identification of genes that can report on aggressiveness prostate tumors and thereby add to information provided by current pathologic analysis. Materials Methods: Expression profiling data from over 100 laser capture microdissection derived samples nonneoplastic epithelium; Gleason patterns 3, 4, 5 node metastasis cancer were used identify at abnormally high levels in only some tumors. These variably overexpressed stratified their...
Genome-wide association studies have identified more than 100 common single nucleotide polymorphisms (SNPs) that are associated with prostate cancer risk. However, the vast majority of these SNPs lie in noncoding regions genome. To test whether risk regulate their target genes through long-range chromatin interactions, we applied capture-based 3C sequencing technology to investigate possible cis-interactions at ten loci six cell lines. We significant physical interactions between and...
Folate and related micronturients may affect colorectal cancer (CRC) risk, but the molecular mechanism(s) remain incompletely defined. We analyzed associations between dietary folate, vitamin B6, B12, methionine with incident CRC, overall by microsatellite instability (MSS/MSI-L or MSI-H), CpG island methylator phenotype (CIMP-negative CIMP-positive), BRAF mutation (negative positive), KRAS positive) status in prospective, population-based Iowa Women's Health Study (IWHS; 55–69 years at...
<h3>Background</h3> Postmenopausal hormone (PMH) therapy may reduce colorectal cancer (CRC) risk, but existing data are inconclusive. <h3>Objectives</h3> To evaluate associations between PMH and incident CRC, overall by molecularly defined subtypes, in the population-based Iowa Women9s Health Study of older women. <h3>Methods</h3> Exposure were collected from participants (55–69 years) at baseline (1986). Archived, paraffin-embedded tissue specimens for 553 CRC cases analysed to determine...
Existing data support a modest association between cigarette smoking and incident colorectal cancer (CRC) overall. In this study, we evaluated associations CRC risk stratified by KRAS mutation status, using tissue resources from the Iowa Women's Health Study (IWHS).The IWHS is population-based cohort study of incidence among 41,836 randomly selected women, ages 55-69 years age at enrollment (1986). Exposure data, including smoking, were obtained self-report baseline. Incident CRCs (n=1,233)...
The methods used for sample selection and processing can have a strong influence on the expression values obtained through microarray profiling. Laser capture microdissection (LCM) provides higher specificity in of target cells compared to traditional bulk tissue methods, but at an increased cost. benefit gained from realized LCM sampling is evaluated this study comparison profiles same-samples using processing.Expression data ten lung adenocarcinoma samples six adjacent normal were acquired...
Multiple genome-wide scans for hereditary prostate cancer (HPC) have identified susceptibility loci on nearly every chromosome. However, few results been replicated with statistical significance. One exception is chromosome 22q, which five independent linkage studies yielded strong evidence a locus in HPC families. Previously, we refined this region to 2.53 Mb interval, using recombination mapping 42 linked pedigrees. We now refine 15 kb spanning Apolipoprotein L3 (APOL3), family-based...
Abstract Background: Postmenopausal hormone (PMH) therapy represents a controversial colorectal cancer (CRC) preventive intervention. Because carcinogenesis is heterogeneous process, we evaluated associations between PMH and incident CRC in relation to KRAS mutation status population-based cohort of older women [Iowa Women's Health Study (IWHS)]. Methods: The IWHS enrolled 41,836 randomly selected women, ages 55 69 years, 1986. other exposure data were recorded at baseline. Tissue samples...
Increased alcohol consumption is a putative colorectal cancer (CRC) risk factor. However, existing data are less conclusive for women than men. Also, to date, relatively few studies have reported alcohol-related CRC risks based on molecularly defined tumor subtypes. We evaluated associations between intake and incident CRC, overall by microsatellite instability [MSI high (MSI-H) or MSI low/microsatellite stable (MSI-L/MSS)], CpG island methylator phenotype (CIMP positive CIMP negative), BRAF...
Cigarette smoking (smoking), hormone therapy (MHT), and folate intake (folate) are each thought to influence colorectal cancer risk, but the underlying molecular mechanisms remain incompletely defined. Expression of estrogen receptor β (ESR2) has been associated with stage survival.In this prospective cohort study, we examined smoking, MHT, folate-associated risks by ESR2 protein expression level among participants in Iowa Women's Health Study (IWHS). Self-reported exposure variables were...
Abstract Cigarette smoking (CS), hormone therapy (HT), and folate intake (FI) are each thought to influence colorectal cancer risk, but the underlying molecular mechanisms remain incompletely defined. The TP53 (p53) protein, encoded by tumor-suppressor gene that is commonly mutated in cancer, can be readily assessed differentiate biologically distinct subtypes. In this prospective cohort study, we examined CS-, HT-, FI-associated risks protein expression level among Iowa Women's Health Study...