We report fabrication and characterization of nanochannel devices with two nanopores in series for resistive-pulse sensing hepatitis B virus (HBV) capsids. The pores are patterned by electron beam lithography between microchannels etched reactive ion etching. 50-nm wide, deep, 40-nm long spaced 2.0-μm apart. that brackets the is 20× wider (1 μm) to reduce electrical resistance adjacent ensure current returns its baseline value events. Average pulse amplitudes differ <2% demonstrate technique...

Bottlebrush polymers contain polymeric side-chains attached to a linear polymer backbone, and they are currently of interest for variety potential applications that include drug delivery, tailored surface wettability, self-assembled photonics. These challenging synthesize in large quantities, so practical applications, it is study their properties as additives low-cost polymers. In this work, we examine the phase behavior bottlebrush polystyrene (PS) deuterated (dPS) thin films. nearly...

Ovarian cancer is the fifth leading cause of cancer-related mortalities for women in United States and most lethal gynecological cancer. Aberrant glycosylation has been linked to several human diseases, including ovarian cancer, accurate measurement changes may provide relevant diagnostic prognostic information. In this work, we used microchip electrophoresis coupled with laser-induced fluorescence detection determine quantitative differences among N-glycan profiles control individuals...

We report analysis of N-glycans derived from disease-free individuals and patients with Barrett's esophagus, high-grade dysplasia, esophageal adenocarcinoma by microchip electrophoresis laser-induced fluorescence detection. Serum samples in 10 μL aliquots are enzymatically treated to cleave the that subsequently reacted 8-aminopyrene-1,3,6-trisulfonic acid add charge a fluorescent label. Separations at 1250 V/cm over 22 cm yielded efficiencies up 700 000 plates for times under 100 s....

To characterize the structures of N-glycans derived from human serum, we report a strategy that combines microchip electrophoresis, standard addition, enzymatic digestion, and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). We compared (i) electrophoretic mobilities known well-characterized (standard) glycoproteins through (ii) with their molecular weights determined by MALDI-MS, (iii) profiles enzymatically treated fucosidase. The key step to identify sialylated...

We designed and fabricated microfluidic devices with serpentine separation channels asymmetrically tapered turns, thus allowing high efficiency separations minimizing band broadening associated the "racetrack" effect. evaluated performance of these by measuring variation in length, electric field strength, taper ratio number turns. N-Glycans derived from ribonuclease B labeled 8-aminopyrene-1,3,6-trisulfonic acid were electrophoretically separated on lengths 11, 18, 22, 36 cm at strengths...

ABSTRACT Controlling the morphology, domain orientation, and size of block copolymer (BCP) thin films is desirable for many applications in nanotechnology. These properties can be tuned during solvent annealing by varying choice degree swelling which affect effective miscibility volume fraction BCP domains. In this work, we demonstrate with a bulk lamellae‐forming BCP, poly(4‐trimethylsilylstyrene‐ ‐ D , L ‐lactide) (PTMSS‐ b ‐PLA), that composition mixture vapors containing cyclohexane...

Significance Adipogenesis is vital for animals to maintain energy balance by storing lipid. As a crucial integrator of nutrient, energy, and metabolite signals, mammalian target rapamycin (mTOR) reportedly regulates adipogenesis. However, conventional cell cultures are limited mimicking in vivo conditions, previous reports do not provide the localization information mTOR during Here, we developed an automated microfluidic large-scale integration platform differentiating human adult stem...

This work describes the mass distribution of all theoretically possibly tryptic peptides made 20 amino acids, up to 3 kDa, with resolution 0.001 Da. We characterize regions between peaks distribution, including gaps (forbidden zones) and low-populated areas (quiet zones). show how shrink over range when they completely disappear. demonstrate that peptide compositions in quiet zones are less diverse than those by eliminating certain types unrealistic may be increased. The is generated using a...

ABSTRACT Over the past 15 years, block copolymer lithography has emerged as its own research field within broader and polymer thin film communities. This distinction is associated with unique requirements set by semiconductor device industry, such low‐defect densities, precise feature registration, complex pattern layouts. To achieve perfection in lithography, surface substrate interactions must be carefully tuned to control domain ordering three dimensions. perspective discusses recent...

Ionized groups carry net charge and thus play a major role in the electrostatic interactions between ligand receptor. However, their ionization states depend on such factors as pH of water phase, with other charges molecules. Therefore, must be predicted prior to application silico screening or docking protocols. A typical virtual protocol searches for new heat compounds by testing hundreds thousands millions small molecules against particular target Differences size receptors ligand, large...

Assessing risk for voluminous legal documents such as request proposal, contracts is tedious and error prone. We have developed "risk-o-meter", a framework, based on machine learning natural language processing to review assess risks of any document. Our framework uses Paragraph Vector, an unsupervised model generate vector representation text. This enables the learn contextual relations terms sensible context aware embedding. The then feeds space into supervised classification algorithm...

The synthesis, characterization, and self-assembly of a series linear poly(ethylene-b-styrene-b-isoprene) (PEO−PS−PI) triblock copolymers containing nearly equal volume fractions PEO PI (fPEO ≈ fPI) various the middle PS block (70.8 vol % ≤ fPS 75.6 %) is reported. A range azide-functionalized poly(ethylene oxide-b-styrene) diblock were prepared by atom transfer radical polymerization (ATRP) styrene using same batch PEO-macroinitiator, followed azide-functionalization....

Improvements in the mass accuracy and resolution of spectrometers have greatly aided spectrometry-based proteomics profiling complex biological mixtures. With use innovative bioinformatics approaches, high information can be used for filtering chemical noise spectral data. Using our recent algorithmic developments, we generated distributions all theoretical tryptic peptides composed 20 natural amino acids with masses limited to 3.5 kDa. Peptide are distributed discretely, well-defined peak...

We report free-solution microchip electrophoresis performed at elevated temperatures and high separation field strengths. used microfluidic devices with 11 cm long channels to conduct separations between 22 (ambient) 45°C strengths from 100 1000 V/cm. To evaluate performance, N-glycans were as a model system labeled 8-aminopyrene-1,3,6-trisulfonic acid impart charge for render them fluorescent. Typically, increased diffusivity higher leads axial dispersion poor performance; however, we...

Pharmacovigilance plays an important role in monitoring safety of pharmaceutical products. There is abundance unstructured data available social media and online reviews that can provide valuable insights into adverse drug reaction (ADR). This research paper aims to explore the efficient techniques pharmacovigilance identifying ADRs from reviews. work proposes use a transformer-based model for ADR classification, followed by named entity recognition (NER) identify causality between drugs...

It is evident from a wide range of experimental findings that ion channel gating inherently stochastic. The issue "memory effects" (diffusional retardation due to local changes in water viscosity) ionic flow has been recently addressed using Brownian dynamics simulations. results presented indicate such memory effects are negligible, unless the diffusional barrier much higher than free solute. In this paper differential stochastic methods we conclude Markovian property exponential dwell...

Label-free shotgun proteomics holds great promise, and has already had some successes in pinpointing which proteins are up or down regulated certain disease states.However, there still pressing issues concerning the statistical analysis of label-free proteomics, this field not enjoyed as much dedication research towards it microarray has.Here we reapply previously used methods, QSpec quasi-Poisson, well apply negative binomial distribution to both a control data set with known differential...

ABSTRACT We investigate the ordering of poly(styrene‐ b ‐methyl methacrylate) (PS‐PMMA) lamellar copolymers (periodicity L 0 = 46 nm) confined between a free surface and brushed r silicon substrate. The processing temperature was selected to eliminate wetting layers at top bottom interfaces, producing approximately neutral boundaries that stabilize perpendicular domain orientations. PS‐PMMA film thickness ( t 0.5 − 2.5 ) brush grafting density (Σ 0.2–0.6 nm −2 were systematically varied...

Abstract We report a method to determine HbA 1c (glycated hemoglobin) where whole blood samples are prepared by fast hemolysis (dilution with deionized water and vortex mixing), digestion 0.6 mg/mL endoproteinase Glu C (Glu C) in 30 mM ammonium acetate buffer (pH 4.3) at 37 °C for 45 min, termination of the diluting 0.1% formic acid water, then analysis gradient liquid chromatography-tandem mass spectrometry (LC–MS/MS) run time 36 s. The is linear between 0 200 /mol Hb (IFCC) correlation...

It is quite clear from a wide range of experiments that gating phenomena ion channels inherently stochastic. has been discussed using BD simulations in recent paper memory effects transport negligible, unless the barrier height high. In this brief report we like to state Differential Stochastic Methods (DSM's) Markovian property exponential dwell times do indeed give rise high barrier, which turn indicates need not be ignored. We have thus constructed Generalized Langevin Equation contains...

Elucidating the structure and/or dynamics of gene regulatory networks from experimental data is a major goal systems biology. Stochastic models have potential to absorb noise, account for un-certainty, and help avoid overfitting. Within frame work probabilistic polynomial dynamical systems, we present an algorithm reverse engineering any network as discrete, system. The resulting stochastic model assembled all minimal in space probability assignment based on partitioning according likeliness...