A5090634359- Bone Metabolism and Diseases
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Rheumatoid Arthritis Research and Therapies
- Blood transfusion and management
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Glycosylation and Glycoproteins Research
- TGF-β signaling in diseases
- Systemic Lupus Erythematosus Research
- Connective tissue disorders research
- Blood Coagulation and Thrombosis Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Bone health and treatments
- Bone and Joint Diseases
- Proteoglycans and glycosaminoglycans research
- Diabetes Management and Research
- MicroRNA in disease regulation
- Caveolin-1 and cellular processes
- Plant-Microbe Interactions and Immunity
- Clinical Laboratory Practices and Quality Control
- Metabolomics and Mass Spectrometry Studies
- Protease and Inhibitor Mechanisms
- Biosimilars and Bioanalytical Methods
- Cancer Cells and Metastasis
- Atherosclerosis and Cardiovascular Diseases
Roche Pharma AG (Germany)
2024
Universitätsklinikum Erlangen
2017-2022
Friedrich-Alexander-Universität Erlangen-Nürnberg
2013-2020
University of Manchester
2019
Philipps University of Marburg
2009
Max Planck Society
1999
Friedrich Schiller University Jena
1999
Max Planck Institute of Biochemistry
1997
The aggregating proteoglycans (aggrecan, versican, neurocan, and brevican) are important components of many extracellular matrices. Their N-terminal globular domain binds to hyaluronan, but the function their C-terminal region containing a C-type lectin is less clear. We now report that 90-kDa protein copurifies with recombinant domains from aggrecan not brain-specific neurocan brevican. Amino acid sequencing tryptic peptides this identified it as fibulin-1. This matrix glycoprotein strongly...
JAK inhibition enhances the function of bone-forming osteoblasts, increases bone mass, and induces repair arthritic erosions.
Abstract Osteoclasts are specialised bone resorbing cells that control both physiological and pathological turnover. Functional changes in the differentiation activity of osteoclasts accompanied by active metabolic reprogramming. However, biological significance vivo relevance these events has remained unclear. Here we show resorption differentiated heavily relies on increased aerobic glycolysis glycolysis-derived lactate production. While pharmacological inhibition did not affect osteoclast...
Abstract Cytokines of the interleukin (IL)-1 family regulate immune and inflammatory responses. The recently discovered IL-36 members are involved in psoriasis, rheumatoid arthritis, pulmonary diseases. Here, we show that IL-36α interacts with heme thereby contributing to its regulation. Based on in-depth spectroscopic analyses, describe two heme-binding sites associate a pentacoordinated fashion. Solution NMR analysis reveals structural features complex heme. Structural investigation...
Fibrinogen is the first coagulation factor becoming critical in dilution coagulopathy. Volume replacement major blood loss performed with large volumes of crystalloid and colloid solutions. The latter has been shown to compromise accurate photo-optical measurement fibrinogen. This study determined influence different hydroxyethyl starch (HES) formulations.Citrated plasma samples 8 healthy volunteers were diluted by 30% or 50% either HES 10% (200/0.5; HES-200), 6% (70/0.5; HES-70), (450/0.7;...
Adequate fibrinogen concentration is a crucial component of sufficient perioperative/posttraumatic hemostasis. In major blood loss, large volumes fluids are being administered, which have been shown to interfere with valid determination concentration. This may lead wrong treatment decisions. We studied the variables that cause discrepancies between measured and true concentrations in samples diluted volume replacement fluids.Citrated plasma healthy volunteers were by 30% 50% phosphate...
Bone morphogenetic proteins (BMPs) are multifunctional cytokines. BMP-6 is involved in early steps of keratinogenesis. Esophageal squamous-cell carcinoma often shows abnormal keratinization. The aim this study was to examine the expression and regulation esophageal determine relationship its with tumor characteristics survival. Avidin-biotin-peroxidase staining performed using paraffin-wax-embedded specimens collected from 172 patients carcinoma, who underwent potentially curative surgery....
The interleukin (IL)-1 family member IL-33 has been described as intracellular alarmin with broad roles in wound healing, skin inflammation but also autoimmunity. Its dichotomy between full length (fl) and the mature (m) form of its release by necrosis is still not fully understood. Here, we compare functional consequences both forms vivo, therefore generated two lines transgenic mice which selectively overexpress mmIL-33 flmIL-33 basal keratinocytes. Transgene mRNA was expressed at high...
Here we show that soluble CD83 induces the resolution of inflammation in an antigen-induced arthritis (AIA) model. Joint swelling and arthritis-related expression levels IL-1β, IL-6, RANKL, MMP9, OC-Stamp were strongly reduced, while Foxp3 was induced. In addition, observed a significant inhibition TRAP + osteoclast formation, correlating with reduced arthritic disease score. contrast, cell-specific deletion human murine precursor cells resulted enhanced formation mature osteoclasts. RNA...
Abstract We report a method to determine HbA 1c (glycated hemoglobin) where whole blood samples are prepared by fast hemolysis (dilution with deionized water and vortex mixing), digestion 0.6 mg/mL endoproteinase Glu C (Glu C) in 30 mM ammonium acetate buffer (pH 4.3) at 37 °C for 45 min, termination of the diluting 0.1% formic acid water, then analysis gradient liquid chromatography-tandem mass spectrometry (LC–MS/MS) run time 36 s. The is linear between 0 200 /mol Hb (IFCC) correlation...
Tiefe Venenthrombosen sind nicht selten, und die Untersuchung einer Gerinnungsneigung oder Thrombophilie gehört zu den häufigsten Anforderungen an das medizinische Labor. Auf diesem komplexen Gebiet ergibt sich Diagnose neben der Klinik oft aus einem Wechselspiel zwischen gerinnungsphysiologischen Funktionstests, biochemischen Analysen molekulargenetischen Untersuchungen. So steht Aufklärung exemplarisch für Rolle Molekulargenetik in Labormedizin. Ziel vorliegenden Übersicht ist es,...
<h3>Career situation of first and presenting author</h3> Student for a master or PhD. <h3>Introduction</h3> Many cytokines relevant to rheumatoid arthritis (RA) rely on the janus kinase – signal transducer activator transcription (JAK-STAT) signaling pathway. The JAK-inhibitors tofacitinib baricitinib, targeting JAK3/JAK1 JAK1/JAK2 respectively, have been approved treatment RA<sup>1</sup>. While currently available therapies reduce inflammation, erosive damage in involved joints is still...
Deep vein thrombosis is not a rare medical condition, and the investigation of clotting disposition or thrombophilia one most common tasks required laboratory.In this complex approach, diagnosis achieved on basis clinical features backed up by combination investigations function, biochemical analyses, genetic tests.Therefore, represents as good example role molecular testing in laboratory medicine.The aim present review to outline current state art investigating with particular reference...
<h3>Background</h3> Targeting cytokines relevant to rheumatoid arthritis (RA) has proven efficient in clinical practice, but there is still demand for therapies that rebuild joint tissues which have been subjected deterioration. Since many involved RA rely on the intracellular janus kinase - signal transducer and activator of transcription (JAK-STAT) signaling pathway, targeting them presents itself as option. For this approach, JAK-inhibitors such Tofacitinib Baricitinib, JAK1/JAK3...
Background: Recent developments of targeted treatments such as synthetic DMARDs (tsDMARDs) increase the chances a sustained low disease activity (LDA) or remission state for patients suffering rheumatoid arthritis (RA). tsDMARDs baricitinib, an oral inhibitor Janus Kinases (JAK1/JAK2) was recently approved treatment RA with inadequate response to conventional (cDMARD) and biological (bDMARD) therapy. (1, 2). Objectives: Aim this study is analyze effect baricitinb on (DAS28, LDA) in real...