A5088443571- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Chemokine receptors and signaling
- Atherosclerosis and Cardiovascular Diseases
- Peptidase Inhibition and Analysis
- Animal Genetics and Reproduction
- Cancer, Lipids, and Metabolism
- Immune cells in cancer
- Virus-based gene therapy research
- Advanced Breast Cancer Therapies
- Immune Response and Inflammation
- Lipid metabolism and disorders
- Cervical Cancer and HPV Research
- Ubiquitin and proteasome pathways
- Cancer Treatment and Pharmacology
- Adipokines, Inflammation, and Metabolic Diseases
- Acute Myocardial Infarction Research
- Cardiovascular Disease and Adiposity
- Extracellular vesicles in disease
- COVID-19 Impact on Reproduction
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Metabolism, Diabetes, and Cancer
- Genetic and phenotypic traits in livestock
Duke University
2024
University of California, San Diego
2019
Nanjing Medical University
2017
Huaian First People’s Hospital
2017
Zhengzhou University
2015
Lung cancer remains one of the most prevalent and deadly malignancies globally, with non-small cell lung (NSCLC) comprising approximately 84% all cases. Despite advances in treatment, overall 5-year survival rate for NSCLC at 15%. Among cases, 15-20% are driven by activating mutations (such as L858R) epidermal growth factor receptor (EGFR), which treated EGFR tyrosine kinase inhibitors (TKIs). Although three generations TKIs have been developed, a number genetic (T790M C797S) shown to evolve...
Abstract The ability to temporally regulate gene expression and track labeled cells makes animal models powerful biomedical tools. However, sudden of xenobiotic genes [e.g., GFP, luciferase (Luc), or rtTA3] can trigger inadvertent immunity that suppresses foreign protein results in complete rejection transplanted cells. Germline exposure antigens somewhat addresses these challenges; however, native fluorescence bioluminescence abrogates the utility reporter proteins highly spatiotemporally...
This study aims to explore the correlation of hepatocyte growth factor (HGF) and fibroblast activation protein (FAP) expressions with angiogenesis metastasis in colorectal cancer (CRC).The immunohistochemical SABC method was used detect HGF FAP 127 CRC tissues, 51 polyp tissues 28 normal tissues.HGF liver were detected using western blot analyze their lymph node metastasis.Micro-vessel density (MVD) clinic-pathologic information patients recorded analyzed.In group, greatly higher than those...
Fondaparinux and enoxaparin are used in patients with acute coronary syndrome (ACS), but their effect particular populations of is not well known. The objective was to explore the difference between fondaparinux non-ST elevation ACS (NSTE-ACS) treated percutaneous intervention (PCI) tirofiban.We prospectively enrolled 461 NSTE-ACS PCI, tirofiban, either (n = 229) or 232). Death, myocardial infarction, recurrent ischaemia its composite outcome were assessed. incidences major minor bleeding...
<div>Abstract<p>The ability to temporally regulate gene expression and track labeled cells makes animal models powerful biomedical tools. However, sudden of xenobiotic genes [e.g., GFP, luciferase (Luc), or rtTA3] can trigger inadvertent immunity that suppresses foreign protein results in complete rejection transplanted cells. Germline exposure antigens somewhat addresses these challenges; however, native fluorescence bioluminescence abrogates the utility reporter proteins highly...
<p>Validating mutant GFP and Luc activity in Adenovirus vaccines</p>
<p>Summary of tumor behavior in differentially tolerant animal models. <b>A,</b> Xenoantigen-bearing cells implanted orthotopically into immune-deficient mice typically grow and metastasize as expected can be easily observed with fluorescence/bioluminescence. <b>B,</b> Conversely, immune-competent animals the presence foreign immunogens often results complete rejection primary tumor, or severely restricts expression immunogenic antigens limits utility reporter...
<p>Additional E0771 tumor growth in WT, GH, and CAG Luc-GFP mice; MMTV HER2 Ad-HER2 vaccination T cell responses.</p>
<p>Mutant GFP and luciferase induce immune responses comparable with wild-type (WT) proteins. <b>A,</b> WT male Balb/c mice were vaccinated Ad encoding or mutant copies of (ΔT64; <i>n</i> = 5/group) serum antibody measured after 3 weeks. Mice showed equivalent anti-GFP whether GFP. <b>B,</b> female C57Bl/6 Ad-GFP-Luc Ad-Luc (G315A; weeks revealing similar levels anti-Luc antibodies. <i>P</i> values for A, B are at 1:50 dilution by two-way...
<p>NoGlow mice reveal the impact of sex and tissue distribution on immune tolerance. <b>A,</b> Male WT (<i>n</i> = 3), CAG Luc-GFP NoGlow+ 6) or NoGlow− 10) littermates were vaccinated with an Ad encoding GFP-Luc serum was collected after two weeks for anti-Luc anti-GFP responses. Unvaccinated naïve 2) animals used as baseline controls. <b>B,</b> Luc staining showing protein expression in mammary epithelium female MMTV Cre littermates....
<p>Tumor cells expressing GFP, rtTA, and Luciferase successfully engraft in NoGlow mice. <b>A,</b> Diagram of triple-transgenic (3 ×) E0771 cells. GFP rtTA are constitutively expressed Luc is induced with the addition doxycycline. <b>B,</b> 3 × (10<sup>6</sup>) were implanted into mammary fat pad (MFP) wild-type (WT) C57Bl/6 (<i>n</i> = 7), CAG-driven Full-body WT GFP/Luc mice CMV cre littermates positive 6) or negative 7) for construct,...
<p>Peptides used for ELISPOTs</p>
<p>Summary of tumor behavior in differentially tolerant animal models. <b>A,</b> Xenoantigen-bearing cells implanted orthotopically into immune-deficient mice typically grow and metastasize as expected can be easily observed with fluorescence/bioluminescence. <b>B,</b> Conversely, immune-competent animals the presence foreign immunogens often results complete rejection primary tumor, or severely restricts expression immunogenic antigens limits utility reporter...
<p>Confirming triple-transgenic GFP rtTA-Luc construct in E0771 cells</p>
<div>Abstract<p>The ability to temporally regulate gene expression and track labeled cells makes animal models powerful biomedical tools. However, sudden of xenobiotic genes [e.g., GFP, luciferase (Luc), or rtTA3] can trigger inadvertent immunity that suppresses foreign protein results in complete rejection transplanted cells. Germline exposure antigens somewhat addresses these challenges; however, native fluorescence bioluminescence abrogates the utility reporter proteins highly...
<p>Tolerance to foreign antigens is animal model specific. <b>A</b> and <b>B,</b> Mice expressing GFP Luc in the pituitary (GH; <i>n</i> = 5), full-body GFP, (CAG Luc-GFP; FoxP3<sup>+</sup> cells (Foxp3-GFP; 4), or wild-type (WT) C57Bl/6 (<i>n</i> 4) were vaccinated with adenovirus (Ad) encoding GFP/Luc (2.6 × 10<sup>10</sup> vp/mouse) anti-Luciferase (A) anti-GFP (B) serum evaluated by ELISA after 2 weeks. Only...
<p>NoGlow mice express eGFP and Luciferase without background fluorescence bioluminescence. <b>A,</b> Diagram of the NoGlow construct. LoxP-flanked stop site prevents expression construct in absence Cre recombinase. However, presence is recombined to yield a single transcript encoding rtTA3 mutant GFP/Luc driven by CAG promoter. <b>B,</b> Chimeric founder animals were crossed CMV (full-body) test activity Representative bioluminescence imaging female C57Bl/6,...
<p>NoGlow mice reveal unappreciated metastatic dynamics independent of the primary tumor. <b>A,</b> Tumor growth triple transgenic (3 ×) B16-F10 cells (10<sup>5</sup>) after subcutaneous implantation into flank WT C57Bl/6 (<i>n</i> = 2), CAG-driven Full-body GFP/Luc expressing 3), CMV cre littermates positive 8) or negative 5) for NoGlow construct, SCID beige mice. <b>B,</b> Bioluminescence imaging lungs at time euthanasia each mouse from...
<p>Description of animal models used in the study</p>
<p>NoGlow mice express eGFP and Luciferase without background fluorescence bioluminescence. <b>A,</b> Diagram of the NoGlow construct. LoxP-flanked stop site prevents expression construct in absence Cre recombinase. However, presence is recombined to yield a single transcript encoding rtTA3 mutant GFP/Luc driven by CAG promoter. <b>B,</b> Chimeric founder animals were crossed CMV (full-body) test activity Representative bioluminescence imaging female C57Bl/6,...
<p>Validating mutant GFP and Luc activity in Adenovirus vaccines</p>