A5060744389- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Advanced NMR Techniques and Applications
- Crystallography and molecular interactions
- Protein Structure and Dynamics
- Advanced Polymer Synthesis and Characterization
- Molecular Sensors and Ion Detection
- Enzyme Structure and Function
- Synthesis and properties of polymers
- Drug Transport and Resistance Mechanisms
- Phytochemicals and Antioxidant Activities
- Phagocytosis and Immune Regulation
- Mass Spectrometry Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- NMR spectroscopy and applications
- biodegradable polymer synthesis and properties
- Sulfur Compounds in Biology
- DNA and Nucleic Acid Chemistry
- Supramolecular Chemistry and Complexes
- Biochemical effects in animals
- RNA and protein synthesis mechanisms
- Folate and B Vitamins Research
- Surfactants and Colloidal Systems
- Carbon dioxide utilization in catalysis
Friedrich Schiller University Jena
2015-2024
University of Zurich
2023
University Hospital of Zurich
2023
Institute of Macromolecular Chemistry
2016-2017
Leibniz Association
2012-2015
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2013-2015
University of Oulu
2012
Circular dichroism (CD) spectroscopy is one of the most versatile tools to study protein folding and validate proper fold purified proteins. Here, we aim provide a readily accessible, user-friendly platform-independent tool capable analysing multiple CD datasets virtually any format returning results as high-quality graphical output user.CAPITO (CD Anaylsis Plotting Tool) novel web server-based for plotting data. It allows reliable estimation secondary structure content utilizing different...
The identification and discrimination of diols is fundamental importance in medical diagnostics, such as measuring the contents glucose urine diabetes patients. Diol sensors are often based on fluorophore-appended boronic acids, but these severely lack discriminatory power their response one-dimensional. As an alternative strategy, we present use fluorinated acid-appended pyridinium salts combination with 19F NMR spectroscopy. A pool 59 (bio)analytes was screened, containing monosaccharides,...
Spinocerebellar ataxia type 3 (SCA3) is pathologically characterized by the formation of intranuclear aggregates which contain ataxin-3, mutated protein in SCA3, a specific subtype neurons. It has been proposed that ataxin-3 cleaved proteolytic enzymes, particular calpains and caspases, eventually leading to aggregates. In our study, we examined ability cleave vitro vivo. We demonstrated cell culture mouse brain homogenates cleavage overexpressed calpain-2 occur polyglutamine expanded more...
Reactive polymersomes represent a versatile artificial cargo carrier system that can facilitate an immediate release in response to specific stimulus. The herein presented oxidation-sensitive feature time-delayed mechanism oxidative environment, which be precisely adjusted by either tuning the membrane thickness or partial pre-oxidation. These polymeric vesicles are conveniently prepared PISA allowing straightforward and effective situ encapsulation of molecules, as shown for dyes enzymes....
A heteroditopic receptor with an iodo-triazole moiety for simultaneous cation–anion binding through Lewis-basic nitrogen atoms and halogen-bond-donating iodine was designed.
Preorganization via intramolecular hydrogen bonds was applied in a cleft-type receptor by exploiting the excellent halogen bond donor ability as well acceptor function of iodo-1,2,3-triazoles. As investigated isothermal calorimetric titrations, restriction conformational freedom causes an enhanced entropic contribution resulting strongly increased binding affinity. This efficient way to improve strength 5-halo-1,2,3-triazoles paves for applications new charge-neutral donors solution.
Diatoms are abundant unicellular microalgae, responsible for ≈20 % of global photosynthetic CO2 fixation. Nevertheless, we know little about fundamental aspects their biology, such as sexual reproduction. Pheromone-mediated chemical communication is crucial successful mating. An attraction pheromone was identified in the diatom Seminavis robusta, but metabolites priming cells sex and synchronizing search mating behavior remained elusive. These sex-inducing pheromones (SIP) induce cell cycle...
The RecQL4 helicase is involved in the maintenance of genome integrity and DNA replication. Mutations human gene cause Rothmund–Thomson, RAPADILINO Baller–Gerold syndromes. Mouse models experiments Xenopus have proven N-terminal part to be vital for cell growth. We identified first 54 amino acids (RecQL4_N54) as minimum interaction region with TopBP1. solution structure RecQL4_N54 was determined by heteronuclear liquid–state nuclear magnetic resonance (NMR) spectroscopy (PDB 2KMU; backbone...
Abstract Cytokines of the interleukin (IL)-1 family regulate immune and inflammatory responses. The recently discovered IL-36 members are involved in psoriasis, rheumatoid arthritis, pulmonary diseases. Here, we show that IL-36α interacts with heme thereby contributing to its regulation. Based on in-depth spectroscopic analyses, describe two heme-binding sites associate a pentacoordinated fashion. Solution NMR analysis reveals structural features complex heme. Structural investigation...
A highly porous cryogel is prepared and subsequently functionalized with an atom transfer radical polymerization (ATRP) initiator at the surface. Two new glycomonomers are introduced, which possess deprotected mannose as well glucose moieties. These copolymerized N-isopropylacrylamide (NiPAm) from surface, providing a hydrophilic material, characterized by SEM, FT-IR spectroscopy, NMR spectroscopy. This support can be applied for affinity chromatography of whole cells owing to high pore...
We present the synthesis and characterization of amphiphilic double hydrophilic block copolymers containing a polydehydroalanine (PDha) block.
Maturity stage affects the bioactive compounds as well antioxidant capacity in fruit. This study was designed to identify and quantify carotenoids, evaluate vitamin E, C, total phenolic of Rosa rugosa hips at different degrees ripeness. HPLC (high performance liquid chromatography) analysis showed types carotenoids stages maturity R. with significant differences (p ˂ 0.05), where maximum concentration observed late harvesting. In investigated, only α-tocopherol detected, both E C obtained...
Detection and discrimination of similar solvation energies bioanalytes are vital in medical practical applications. Currently, various advanced techniques equipped to recognize these crucial bioanalytes. Each strategy has its own benefits limitations. One-dimensional response, lack power for anions, reactive oxygen species (ROS) generally limit the utilized fluorescent probe. Therefore, a cutting-edge, refined method is expected conquer The use
The synthesis of poly(2-oxazoline)-based block copolymers consisting a cationic and hydrophilic segment is described. self-assembly these macromolecules in organic solvents results the formation micelles vesicles, respectively, depending on solvent used. To transfer systems into water, cross-linking using glutaraldehyde was applied, followed by consumption excessive aldehyde functions either diethylamine or 6-aminofluorescein (6AF). cross-linked assemblies were analyzed regarding their size...
(Bio)degradable poly(ethylene imine-co-glycine)s (P(EI-co-Gly)) were synthesized through efficient and controlled oxidation of linear imine) (LPEI) using hydrogen peroxide in a methanol/water mixture. Temperature, concentration, reaction time varied to adjust the degree (DO). At low temperatures, process was found be well-controlled with almost 85% ethylene imine units converted corresponding amide. Importantly, more than 10% LPEI rendered polymers water-soluble. The molecular structure...
Cystathionine-β-synthase (CBS) belongs to a large family of pyridoxal 5'-phosphate (PLP)-dependent enzymes, responsible for the sulfur metabolism. The heme-dependent protein CBS is part regulatory pathways also involving gasotransmitter hydrogen sulfide. Malfunction can lead pathologic conditions like cancer, cardiovascular and neurodegenerative disorders. Truncation residues 1-40, absent in X-ray structures CBS, reduces but does not abolish activity enzyme. Here we report NMR resonance...
Four endogenous products of oxidative bilirubin degradation were isolated and fully characterized. The constitutional isomers belong to the propentdyopents (PDPs). Their structures further transformations biologically active oxidation end (Z-BOXes) are reported. Using liquid chromatography–mass spectrometry protocols, PDPs detected in human bile gallstones. Given recent interest BOXes as effectors cerebral vasospasms liver dysfunction, co-occurring represent an additional potentially...
Abstract The hydrophosphorylation of phenylacetylene with di(aryl)phosphane oxides Ar 2 P(O)H (Pudovik reaction) yields E / Z ‐isomer mixtures phenylethenyl‐di(aryl)phosphane ( 1 ). Alkali and alkaline‐earth metal di(aryl)phosphinites have been studied as catalysts for this reaction increasing activity the heavier s‐block metals. Pudovik can only be mediated whereas P‐bound alkyl alcoholate substituents impede P−H addition across alkynes. demanding mesityl group favors...
Biomimetic hydrogels are advanced biomaterials that have been developed following different synthetic routes. Covalent postfabrication functionalization is a promising strategy to achieve efficient matrix modification decoupled of general material properties. To this end, dual-functional macromers were synthesized by free radical polymerization maleic anhydride with diacetone acrylamide (N-(1,1-dimethyl-3-oxobutyl)acrylamide) and pentaerythritol diacrylate monostearate. Amphiphilic oligomers...
The novel lactide isomer 3-ethyl-1,4-dioxane-2,5-dione (3-ethylglycolide, EtGly) represented the basis for development of polyesters varying crystallinity.