Oral cancer is one of the most common and lethal cancers in world. Combination chemotherapy coupled with nanoparticle drug delivery holds substantial promise therapy. This study aimed to evaluate efficacy safety two dosages our novel pH temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NPs) attention MMP-2 mRNA profile a 4-nitroquinoline-1-oxide induced oral squamous cell carcinoma (OSCC) model rat. Our results showed that both IV DOX-MTX NP caused significant...

Human papillomavirus infections are associated with most cervical cancers, which the fourth common cancer in women. HPV-E6 protein binds to p53 and inhibits its function, leading switching of normal cells toward cells. Here, we disrupted gene investigated effects on proliferation apoptosis HeLa The HPV18-E6 was targeted two designed sgRNAs cloned into an AAV-CRISPR-based plasmid. AAV-E6-CRISPR/Cas9 virions were prepared titrated HEK293t cleavage created detected using T7E1 assay. Cell cycle...

Background: Oral squamous cell carcinoma (OSCC) remains as one of the most difficult malignancies to control because its high propensity for local invasion and cervical lymph node dissemination.The aim present study was evaluate efficacy novel pH temperature sensitive doxorubicin-methotrexateloaded nanoparticles (DOX-MTX NP) in terms their potential change VEGF-C expression profile a rat OSCC model.Materials Methods: 120 male rats were divided into 8 groups 15 animals administrated with...

Prostate cancer (PC) is one of the most abundant cancers among men, and In Iran, has been responsible for 6% all deaths from in men. NUF2 GMNN genes are considered as loci susceptibility to tumorigenesis humans. Alterations expression these have reported various malignancies. The aim our study was test whether different levels associated with PC incidence hence, might be new molecular tools screening.Biopsy samples 40 patients 41 healthy Iranian men were used determine relative gene...

Background: Oral squamous cell carcinoma (OSCC) is the sixth most common malignancy worldwide. Cancer development and progression require inactivation of tumor suppressor genes activation proto-oncogenes. The well recognized mechanism action demonstrated for chemotherapeutic agents induction apoptosis via reactivation p53. In this context, we evaluate efficacy IV oral routes our novel PH temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) in affecting p53 profile...

Oral squamous cell carcinoma (OSCC) remains as one of the most difficult malignancies to control because its high propensity for local invasion and cervical lymph node dissemination. In this study, we evaluate efficacy our novel pH temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) in affecting HER2 expression profile OSCC model rat.DOX-MTX- nanoparticle complexes caused significant decrease mRNA level compared untreated cancers (p<0.05) finding was more...

Oral carcinoma (OC) remains one of the most difficult malignancies to cure. Hesa-A is an Iranian herbal-marine compound that has shown promising anti-tumor properties against various human tumors. However, its mechanism action be addressed. The present study was conducted evaluate effect two doses on mRNA expression erb\b2 as a main prognosticator tumor marker for OC in animal model.A total 60 rats were randomly divided into 5 groups 12 animals each. Rats received 0, 250 and 500 mg/kg body...

Background: While the role of adipocyte-derived hormones in breast cancer pathogenesis well studied, it is speculated that there a crosstalk between adipocytokines and Esterogen receptor(ER) signaling. To test this hypothesis we evaluate possible correlation serum levels adipocytokins with steroid hormone receptors gene expression patients. Methods: In case-control study, 70 women participated different grades obesity (36 none obese, BMI 0.05). case leptin, observed positive leptin ERα PR...

Human leukocyte antigen-G (HLA-G) is a non-classical class I molecule highly expressed by extravillous cytotrophoblast cells. Due to single base pair deletion, its function can be compensated other isoforms. Investigating the frequency of null allele in Recurrent Miscarriage (RM) subjects could useful understanding relationship between this and RM given population.This study aimed determine HLA-G*0105N potential association with down-regulation HLA-G RM.Western blotting was used assess level...

Aim: Siglec-8 is exclusively expressed on mast cells, eosinophils and basophils. Possible association of six siglec-8 single nucleotide polymorphisms (SNPs) with susceptibility to allergic asthma in the Azeri population Iran was investigated this study. Materials & methods: A total 194 patients 190 normal subjects were enrolled. PCR strand conformation polymorphism (PCR-SSCP) used determine genotypes studied SNPs. Results: The rs36498 showed significant (odds ratio [OR]: 0.65; p = 0.022) T...

Tuberculosis (TB) is the leading cause of morbidity and mortality among chronic infectious diseases.The goal this cross-sectional study (2011-2013;2013) was to examine patterns TB drug resistance HIV-negative pulmonary patients in regions near Iranian border.To end, MTB isolates were harvested from 300 HIV-negative, smear-positive northwest west border provinces. Isolates subjected first second-line susceptibility testing by 1% proportion method. Demographic clinical data provided using a...

Human double minute2 (hdm2) level increases in most human malignancies. Therefore, inhibition of tumor growth and also induction radiosensitivity may be provided by hdm2 inhibitors. The effects hdm2-siRNA on protein expression, cell apoptosis rate, esophageal squamous carcinoma (ESCC) were studied.The gene was silenced TE1, TE8, TE11 ESCC lines using 200nM siRNA liposomal transfection method followed irradiation with 0.5, 1, 2, 4, 6 Gy γ-rays vitro. expression levels evaluated real time PCR...

Because mothers and fathers are more or less dissimilar at multiple HLA loci, mother considers her fetus as a semi-allograft. Mother's immune system may recognize paternal foreign antigen develop anti-paternal antibodies cytotoxic T lymphocyte. There some mechanisms that modulate maternal responses during pregnancy, in order to make uterus an privileged site. This immunosuppression is believed be mediated, least partly, by HLA-G, non-classical class I human leukocyte (HLA) molecule strongly...

Identifying Mycobacterium tuberculosis (MTB) transmission type is a key step in the control of this disease.This study aimed to determine path and MTB insertion sequence IS6110 band number verify their relationship demographic clinical risk factors.In cross-sectional study, 64 patients from three border provinces Iran were selected after full history physical evaluation design. The drug susceptibility testing was carried out using standard proportion technique on sputum samples. Isolates...

Accurate and rapid detection of drug-resistant Mycobacterium tuberculosis is fundamental for the successful treatment (TB).The aim this study was to determine frequency common mutations leading isoniazid (INH) rifampicin (RMP) resistance.In a cross-sectional carried out in 2014, 90 patients with M. from five border provinces Iran were selected. After full clinical history physical evaluation, real-time polymerase chain reaction (PCR) technique performed patients' katG rpoB genes. The results...

Clustering of and determining Mycobacterium tuberculosis (MTB) strains is great concern in control programs (TB). Identification transmission type tracking the infection source also highly necessary. The aim present study to track determine MTB infection, as well its relationship with demographic factors using PGRS-RFLP. In this cross-sectional study, 84 smear-positive patients from 5 frontier provinces (East Azerbaijan, West Ardebil, Kurdistan, Kermanshah) were investigated according...

Background: Oral carcinoma (OC) remains as one of the most difficult malignancies to cure. Hesa-A is an Iranian herbal-marine compound that has shown promising anti-tumor properties on various human cancer cells, although mechanisms action remain be addressed. This study was conducted evaluate effect two doses mRNA expression p53 a main prognosticator OC. Materials and Methods: 60 rats were randomly divided into 5 groups 12 animals each. Rats in received 0, 250 500 mg/kg body weight three...

Objective. Rapid detection of mycobacterium tuberculosis (MTB) resistance is a key step in the control disease. This study aimed to identify prevalence and accuracy isoniazid (INH) rifampin (RMP) their risk factors using multiplex allele-specific polymerase chain reaction (MAS-PCR) detect such mutations. Materials methods. In this cross-sectional (2012–2013), 257 MTB patients from five frontier border provinces Iran were selected after divulging full clinical history undergoing physical...

The aim of this study was to investigate the influence interleukin (IL)-6 gene polymorphisms on upper respiratory tract infection (URTI) incidence. To end, 100 healthy elite male athletes participating in were classified as either or prone frequent URTI. Blood samples and DNA isolation, multiplex polymerase chain reaction, Taqman real-time reaction carried out. Genomic extracted from peripheral leukocytes whole blood using QIAmp Mini Kit according manufacturer's protocols. For comparison...

Multidrug-resistant tuberculosis (MDR-TB) has emerged as an important global health concern and is on the rise throughout world.The aim of this study was to examine epidemiology pattern TB drug resistance.In cross-sectional study, 180 pulmonary patients from two Northwestern provinces Iran were selected. The first second line susceptibility testing carried out using 1% proportion method Lφwenstein-Jensen medium. Full demographic, environmental clinical history evaluated.Prevalence resistance...