Cervical cancer is a human papilloma virus (HPV)-related cancer, but most HPV infections are transient or intermittent and resolve spontaneously. Thus, other factors, such as cervical microflora, which dominated by lactobacilli, must be involved in invasive carcinoma development after infection. Previous studies have demonstrated that lactobacilli antitumour effects, it possible vaginal prevent cancer. Here we examined the proliferative apoptotic responses of normal tumour cells to common...

Long non-coding RNAs (lncRNAs) and exosomes have been regarded as components of cell signal transmission that modulate indigenous cellular microenvironments. Exosomes also participate in relocation functional lncRNAs between cells.In the present study, we evaluated expression LINC00355, LINC00958, UCA1-201, UCA1-203, MALAT1 urinary isolated from transitional carcinoma (TCC) bladder, non-malignant disorders, normal subjects.LINC00355, was significantly higher TCC patients compared to controls...

Lactobacilli are a group of probiotics with beneficial effects on prevention cancer. However, there is scant data in relation the impacts late-stage cancer progration, especially metastasis. The present original work was aimed to evaluate anti-metastatic and anti-proliferative activity lactobacillus rhamnosus supernatant (LRS) crispatus (LCS) human cervical colon adenocarcinoma cell lines (HeLa HT-29, respectively).In this experimental study, activities LRS LCS were determined through MTT...

Oxacillin resistance was present in 99 of 277 (36%) consecutive Staphylococcus aureus isolates collected from hospital patients Tehran during a 15-month period (January 2004-March 2005). The majority (77/99 = 78%) had been cultured wounds or blood. staphylococcal cassette chromosome mec (SCCmec) types and antimicrobial susceptibility patterns methicillin-resistant S. (MRSA) strains were determined. Disk diffusion agar dilution methods used to determine the agents as instructed by Clinical...

Lactobacilli are probiotics shown to have antitumor activities. In addition, they can regulate gene expression through epigenetic mechanisms. this study, we aimed assess anti tumor activities of Lactobacillus acidophilus and crispatus on the MDA-MB-231 breast cancer cell line. The effects culture supernatants were determined by MTT [3-(4,5-dimethylthiazol-2-y-2,5-diphenyltetrazolium bromide] assay. Changes in 5 cancer-testis antigens (CTAs), namely AKAP4, ODF4, PIWIL2, RHOXF2 TSGA10,...

MicroRNAs (miRNAs) are short non-coding RNA molecules characterized by their regulatory roles in cancer and gene expression. We analyzed the expression of miR-21, miR-205, miR-342 59 patients with breast cancer. Samples were divided into three different groups according to immunohistochemistry (IHC) classification: ER-positive and/or PR-positive group (ER+ PR+; I); HER2-positive (HER2+; II); ER/PR/HER2- negative (ER-/PR-/HER2-; III) as triple group. The levels 3 miRNAs tumor samples compared...

Abstract Adoptive transfer of T cells expressing chimeric antigen receptors (CARs) is considered to be a novel anticancer therapy. To date, in most cases, single‐chain variable fragments (scFvs) murine origin have been used CARs. However, this structure has limitations relating the potential immunogenicity mouse antigens humans and relatively large size scFvs. For first time, we camelid nanobody (VHH) construct CAR against prostate specific membrane (PSMA). The PSMA (NBP) was show...

Journal Article Elevated expression levels of testis‐specific genes TEX101 and SPATA19 in basal cell carcinoma their correlation with clinical pathological features Get access S. Ghafouri‐Fard, Ghafouri‐Fard Department Medical Genetics, Faculty Medicine, Tehran University Sciences, Tehran, Iran Search for other works by this author on: Oxford Academic Google Scholar A. Abbasi, Abbasi H. Moslehi, Moslehi Dermatology, Razi Hospital, N. Faramarzi, Faramarzi Taba taba Vakili, Vakili M.B....

Breast cancer is the most common malignancy in women around world so finding new biomarkers for early detection and also study on molecular aspects of breast valuable. Cancer testis genes are a group expressed solely range human malignancies.In this we determined expression Tsga10, TEX101 ODF3 patients with cancer.Fifty were enrolled study. cell lines MCF-7 MDA-MB-231 used to determine genes. For both lines, TSGA10, by RT-PCR. The presence auto antibody against these patients' serums was...

Cancer stem cell (CSC) markers have attracted considerable attention in tumor diagnostic, prognostic, and therapeutic implications. Detection of cancer cells circulating blood using has received remarkable recently. In this study, we aimed to investigate the messenger RNA (mRNA) expression level Lgr5 DCLK1 as most proposed colorectal CSC circulation also determine subsequent association patients' clinical pathological findings. Peripheral mononuclear (PBMCs) 58 patients with at stage I-IV 33...

SUMMARY Sperm mRNAs could be used as a predictor of fertilization capacity since the transcriptional profile gamete is critical for production viable human sperm. The aim this study was to determine if PRM1, PRM2 , and TNP2 transcripts in spermatozoa from normozoospermic teratozoospermic men correlate with sperm morphology and/or assisted‐reproduction outcomes. Human ejaculates were collected 138 referred an infertility clinic, separated two groups, (n = 72) 66), based on World Health...

Several studies have shown that testis-specific gene antigen (TSGA10) could be considered as a cancer testis (CTA), except for one study which has identified it tumor suppressor gene. In order to exert its function, TSGA10 interacts closely with hypoxia inducible factor (HIF-1α) and since this interaction is still not completely defined, the exact role of in angiogenesis invasion also under question. The current was conducted investigate function evaluate potential effects on invasion. To do...

Abstract Background β‐thalassemia comprises a major group of human genetic disorders involving decrease in or an end to the normal synthesis β‐globin chains hemoglobin. KLF1 is key regulatory molecule involved γ‐ gene switching process directly inducing expression and indirectly repressing γ‐globin. The present study aimed investigate ability engineered CRISPR/ Cas9 system with respect disrupting inhibit β‐hemoglobin K562 cells. Methods We targeted three sites on gene, two which are upstream...

Polysaccharides could be used as biodegradable and biocompatible polymers for scaffolds carriers matrix. Numerous algal, fungi herbal polysaccharides can attenuate degradation of skin matrix by the inhibition elastase, collagenase metalloproteinases (MMPs). In this study, we investigate anti-elastase anti-collagenase potential Lactobacilli exopolysaccharides (LEPS) on normal human fibroblast. Among 60 isolated from plants dairy products, selected LEPS showed high (up to 100%), 87%)...