A5079687770- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Polyamine Metabolism and Applications
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- HIV Research and Treatment
- Viral-associated cancers and disorders
- RNA Interference and Gene Delivery
- Muscle Physiology and Disorders
- Digestive system and related health
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Gut microbiota and health
- Liver Disease Diagnosis and Treatment
- Amino Acid Enzymes and Metabolism
- Cannabis and Cannabinoid Research
- Exercise and Physiological Responses
- Lipid metabolism and disorders
- Molecular Biology Techniques and Applications
- Diet, Metabolism, and Disease
- Muscle metabolism and nutrition
- Public Health and Nutrition
- Metabolism, Diabetes, and Cancer
- Advanced Proteomics Techniques and Applications
Duke-NUS Medical School
2019-2022
National University of Singapore
2018
Agency for Science, Technology and Research
2013-2014
Thyroid hormone receptor β1 (THRB1) and estrogen-related α (ESRRA; also known as ERRα) both play important roles in mitochondrial activity. To understand their potential interactions, we performed transcriptome ChIP-seq analyses found that many genes were co-regulated by THRB1 ESRRA involved metabolic pathways. These included oxidative phosphorylation (OXPHOS), the tricarboxylic acid (TCA) cycle, β-oxidation of fatty acids. TH increased expression activity a THRB1-dependent manner through...
Background: Nonalcoholic steatohepatitis (NASH) is characterized by hepatic steatosis, lobular inflammation, and fibrosis. Thyroid hormone (TH) reduces steatosis; however, the therapeutic effect of TH on NASH-associated inflammation fibrosis not known. This study examined during NASH investigated THs molecular actions autophagy mitochondrial biogenesis. Methods: HepG2-TRβ cells were treated with bovine serum albumin-conjugated palmitic acid (PA) to mimic lipotoxic conditions in vitro. Mice...
Spermidine is a natural polyamine that has health benefits and extends life span in several species. Deoxyhypusine synthase (DHPS) deoxyhypusine hydroxylase (DOHH) are key enzymes utilize spermidine to catalyze the post-translational hypusination of translation factor EIF5A (EIF5AH). Here, we have found hepatic DOHH mRNA expression decreased patients mice with non-alcoholic steatohepatitis (NASH), cells treated fatty acids. The mouse cell culture models NASH concomitant decreases Eif5aH...
Hepatic macroautophagy/autophagy and fatty acid metabolism are transcriptionally regulated by nuclear receptors (NRs); however, it is not known whether their transcriptional co-activators involved in autophagy. We thus examined MED1 (mediator complex subunit 1), a key component of the Mediator Complex that directly interacts with NRs, on these processes. found knockdown (KD) cultured hepatic cells decreased autophagy mitochondrial activity was accompanied transcription genes Lipophagy...
Abstract Skeletal muscle (SM) weakness occurs in hypothyroidism and resistance to thyroid hormone α (RTHα) syndrome. However, the cell signaling molecular mechanism(s) underlying under these conditions is not well understood. We thus examined role of receptor (TRα), predominant TR isoform SM, on autophagy, mitochondrial biogenesis, metabolism demonstrate two conditions. Two genetic mouse models were used this study: TRα1PV/+ mice, which express mutant Thra1PV gene ubiquitously,...
// Qiao Jing Lew 1,* , Kai Ling Chu Yi Chia 1 Benjamin Soo Jia Pei Ho Chew Har Ng Hui Si Kwok Cheng-Ming Chiang 2 Yao Chang 3 and Sheng-Hao Chao 1,4 Expression Engineering Group, Bioprocessing Technology Institute, Agency for Science, Research (A*STAR), Singapore Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Harry Hines Boulevard, Dallas, TX, USA National Institute Infectious Diseases Vaccinology, Health Institutes, Tainan, Taiwan 4 Department Microbiology,...
Although both exercise and thyroid hormone (TH) status can cause cellular metabolic changes in skeletal muscle, the impact of TH on exercise-associated is not well understood. Here, we examined effects muscle fiber type, cell signaling, metabolism a rabbit model training — chronic motor nerve stimulation (CMNS). Five rabbits were rendered hypothyroid for seven to eight weeks three made hyperthyroid two prior CMNS left peroneal ten days. We then measured protein markers autophagy, nutrient-...
<h3>Background</h3> Psychological stress, which is associated with poor sleep quality, has a profound effect on the gut microbiome. To better elucidate microbiome changes psychological we measured levels of stress and quality among healthy stool donors recruited through national campaign, further assessed their relationship <h3>Methods</h3> The microbial composition 272 adults residing in Singapore was determined by 16S rRNA V3-V4 amplicon sequencing. Stress distress participants were using...
<h3>Background</h3> Singapore and Malaysia are neighbouring countries with similar ethnic make-up: Chinese, Indian, Malays. However, is a high-income economy while middle-income economy. Such difference has resulted in some levels of dichotomy diet lifestyles. In this study, we sought to investigate the gut-ethnic variation across two varying economic development. <h3>Methods</h3> A total 439 relatively healthy Malaysian (n=190) Singaporean (n=249) adults (>18) were included, comprising...