A5077469833- Mesenchymal stem cell research
- RNA modifications and cancer
- Cancer-related gene regulation
- Hematopoietic Stem Cell Transplantation
- Glycogen Storage Diseases and Myoclonus
- Adipose Tissue and Metabolism
- Genetics and Neurodevelopmental Disorders
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- Lysosomal Storage Disorders Research
- Neonatal Respiratory Health Research
- Pancreatic function and diabetes
- Cancer-related molecular mechanisms research
- Exercise and Physiological Responses
- Healthcare Systems and Public Health
- Thyroid Disorders and Treatments
- Studies on Chitinases and Chitosanases
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- Cancer therapeutics and mechanisms
- Disaster Response and Management
- Diet, Metabolism, and Disease
- Tree Root and Stability Studies
Seagen (United States)
2023
Duke-NUS Medical School
2014-2021
National University of Singapore
2015-2019
Fred Hutch Cancer Center
2018-2019
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2018
Cancer Research Center
2018
University of Washington
2018
Singapore General Hospital
2011-2013
Pepperdine University
2012
The University of Sydney
2010
Aging causes a general decline in cellular metabolic activity, and function different tissues whole body homeostasis.However, the understanding about metabolomic autophagy changes skeletal muscle heart during aging is still limited.We thus examined markers for macroautophagy, chaperone-mediated (CMA), mitochondrial quality control, as well metabolites cardiac from young (5 months old) aged (27 mice.We found decreased autophagic degradation of p62 increased ubiquitinated proteins both mice,...
Abstract Many of the regulatory features governing erythrocyte specification, maturation, and associated disorders remain enigmatic. To identify new regulators erythropoiesis, we utilize a functional genomic screen for genes affecting expression erythroid marker CD235a/GYPA. Among validating hits are coding N 6 -methyladenosine (m A) mRNA methyltransferase (MTase) complex, including, METTL14 , METTL3 WTAP . We demonstrate that m A MTase activity promotes gene programs through selective...
Background: Glycogen storage disease type Ia (GSD Ia), also known as von Gierke disease, is the most common glycogen disorder. It caused by deficiency of glucose-6-phosphatase, enzyme that catalyzes final step gluconeogenesis and glycogenolysis. The accumulation glucose-6-phosphate leads to increased triglyceride levels in liver. Patients with GSD can develop steatohepatitis, cirrhosis, risk for hepatocellular adenomas carcinomas. We previously showed animal models had defective autophagy...
Abstract Glycogen storage disease type Ia (GSDIa, von Gierke disease) is the most common glycogen disorder. It caused by deficiency of glucose-6-phosphatase, an enzyme which catalyses final step gluconeogenesis and glycogenolysis. Clinically, GSDIa characterized fasting hypoglycaemia hepatic triglyceride overaccumulation. The latter leads to steatohepatitis, cirrhosis, formation adenomas carcinomas. Currently, little known about function various organelles their impact on metabolism in...
Titin-truncating variants (TTNtv) are the most common genetic cause of dilated cardiomyopathy. TTNtv occur in ~1% general population and causes subclinical cardiac remodeling asymptomatic carriers. In rat models with either proximal or distal TTNtv, we previously showed altered metabolism at baseline impaired function response to stress. However, molecular mechanism(s) underlying these effects remains unknown. current study, used investigate effect on autophagy mitochondrial function, which...
Glucose-6-phosphatase α (G6Pase) deficiency, also known as von Gierke's Disease or Glycogen storage disease type Ia (GSD Ia), is characterized by decreased ability of the liver to convert glucose-6-phosphate glucose leading glycogen accumulation and hepatosteatosis. Long-term complications GSD include hepatic adenomas carcinomas, in association with suppression autophagy liver. The G6pc-/- mouse canine models for were treated pan-peroxisomal proliferator-activated receptor agonist,...
A dynamic biosensor which switches conformation according to its methylation status enables highly sensitive detection of m<sup>6</sup>A-demethylase activity. This strategy may be adapted a broad range RNA-modifying enzymes.
Hepatic macroautophagy/autophagy and fatty acid metabolism are transcriptionally regulated by nuclear receptors (NRs); however, it is not known whether their transcriptional co-activators involved in autophagy. We thus examined MED1 (mediator complex subunit 1), a key component of the Mediator Complex that directly interacts with NRs, on these processes. found knockdown (KD) cultured hepatic cells decreased autophagy mitochondrial activity was accompanied transcription genes Lipophagy...
Abstract Skeletal muscle (SM) weakness occurs in hypothyroidism and resistance to thyroid hormone α (RTHα) syndrome. However, the cell signaling molecular mechanism(s) underlying under these conditions is not well understood. We thus examined role of receptor (TRα), predominant TR isoform SM, on autophagy, mitochondrial biogenesis, metabolism demonstrate two conditions. Two genetic mouse models were used this study: TRα1PV/+ mice, which express mutant Thra1PV gene ubiquitously,...
Gastric cancer (GC) is the second leading cause of global mortality worldwide. However, molecular mechanism underlying its carcinogenesis and drug resistance not well understood. To identify novel functionally important genes that were differentially expressed due to combinations genetic epigenetic changes, we analyzed datasets containing genome-wide mRNA expression, DNA copy number alterations methylation status from 154 primary GC samples 47 matched non-neoplastic mucosa tissues Asian...
Although both exercise and thyroid hormone (TH) status can cause cellular metabolic changes in skeletal muscle, the impact of TH on exercise-associated is not well understood. Here, we examined effects muscle fiber type, cell signaling, metabolism a rabbit model training — chronic motor nerve stimulation (CMNS). Five rabbits were rendered hypothyroid for seven to eight weeks three made hyperthyroid two prior CMNS left peroneal ten days. We then measured protein markers autophagy, nutrient-...
Patch clamp analyses of the voltage-gated channels in sensory hair cells isolated from a variety species have been described previously1-4 but this video represents first application those techniques to zebrafish. Here we demonstrate method isolate healthy, intact all inner ear end-organs: saccule, lagena, utricle and semicircular canals. Further, diversity cell size morphology give an example kinds patch recordings that can be obtained. The advantage use zebrafish model system over others...
Abstract Many of the regulatory features governing erythrocyte specification, maturation, and associated disorders remain enigmatic. To identify new regulators erythropoiesis, we performed a functional genomic screen for genes affecting expression erythroid marker CD235a/GYPA. Among validating hits were coding N 6 -methyladenosine (m A) mRNA methyltransferase (MTase) complex, including, METTL14 , METTL3 WTAP . We found that m A MTase activity promotes gene programs lineage specification...
Population Medicine considers the following types of articles:• Research Papers -reports data from original research or secondary dataset analyses.• Review -comprehensive, authoritative, reviews within journal's scope.These include both systematic and narrative reviews.• Short Reports -brief reports research.• Policy Case Studies articles on policy development at a regional national level.• Study Protocols -articles describing protocol study.• Methodology -papers that present different...
Alzheimer's disease (AD) and type 2 diabetes (T2DM) are characterized by amyloid deposition: aggregation of amylin in T2DM is associated with loss insulin-secreting beta-cells, Abeta AD brain neuronal loss. In addition to Abeta, tau deposition characterizes the brain, however it only partly understood how impairs functions. We applied quantitative proteomics miRNA analysis as a discovery tool vitro vivo models, followed functional validation. (1) Quantitative iTRAQ (isobaric tag for relative...