A5083194294- Food composition and properties
- Phytase and its Applications
- Wheat and Barley Genetics and Pathology
- Biofuel production and bioconversion
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Lipid metabolism and biosynthesis
- Genetic diversity and population structure
- Rice Cultivation and Yield Improvement
- Vibrio bacteria research studies
- Plant Disease Resistance and Genetics
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Plant tissue culture and regeneration
- Plant Micronutrient Interactions and Effects
- Mosquito-borne diseases and control
- Plant nutrient uptake and metabolism
- Genetics and Plant Breeding
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- Insect Pest Control Strategies
- Antifungal resistance and susceptibility
Monash University Malaysia
2016-2025
Commonwealth Scientific and Industrial Research Organisation
2005-2024
Agriculture and Food
2020-2024
Sylhet MAG Osmani Medical College
2022
Ministry of Local Government, Rural Development and Co-operatives
2019-2021
Australian Regenerative Medicine Institute
2014
Plant Industry
2004-2013
Monash University
2011-2013
Animal, Food and Health Sciences
2006-2010
Main Roads Western Australia
2008
Foods high in resistant starch have the potential to improve human health and lower risk of serious noninfectious diseases. RNA interference was used down-regulate two different isoforms starch-branching enzyme (SBE) II (SBEIIa SBEIIb) wheat endosperm raise its amylose content. Suppression SBEIIb expression alone had no effect on content; however, suppression both SBEIIa resulted containing >70% amylose. When grain fed rats a diet as wholemeal, several indices large-bowel function, including...
The Hardness (Ha) locus controls grain hardness in hexaploid wheat (Triticum aestivum) and its relatives Aegilops species) represents a classical example of trait whose variation arose from gene loss after polyploidization. In this study, we investigated the molecular basis evolutionary events observed at by comparing corresponding sequences diploid, tertraploid, species Aegilops). Genomic rearrangements, such as transposable element insertions, genomic deletions, duplications, inversions,...
Abstract No studies have investigated the influence of ethnicity in a multi-ethnic middle-income country with long-standing history co-habitation. Stool samples from 214 Malaysian community members (46 Malay, 65 Chinese, 49 Indian, and 54 Jakun) were collected. The gut microbiota participants was using 16S amplicon sequencing. Ethnicity exhibited largest effect size across (PERMANOVA Pseudo-F = 4.24, R 2 0.06, p 0.001). Notably, on retained even after controlling for all demographic, dietary...
Analysis of barley shrunken grain mutants has identified lines with a novel high amylose starch phenotype. The causal mutation is located at the sex6 locus on chromosome 7H, suggesting synthase IIa (ssIIa) gene as candidate altered by mutation. Consistent this hypothesis, no evidence SSIIa protein expression in either granule or soluble fractions endosperm was found. Sequences gene, ssIIa, from three independent showed presence stop codon preventing translation ssIIa transcript each line....
The inactivation of starch branching IIb (SBEIIb) in rice is traditionally associated with elevated apparent amylose content, increased peak gelatinization temperature, and a decreased proportion short amylopectin branches. To elucidate further the structural functional role this enzyme, phenotypic effects down-regulating SBEIIb expression endosperm were characterized by artificial microRNA (amiRNA) hairpin RNA (hp-RNA) gene silencing. results showed that silencing grains did not affect...
The roles of starch branching enzyme (SBE, EC 2.4.1.18) IIa and SBE IIb in defining the structure amylose amylopectin barley (Hordeum vulgare) endosperm were examined. Barley lines with low expression or IIb, both isoforms generated through RNA-mediated silencing technology. These enabled study role each these determining content, distribution chain lengths, frequency amylopectin. In where reduced by >80%, a high phenotype (>70%) was observed, while reduction either alone had minor impact on...
Abstract Type II diabetes is a major chronic disease. In developing countries, the prevalence of type increasing enormously. Much research indicates that choice carbohydrates, particularly those with low glycaemic index (GI) able to assist in management or prevention diabetes. Most countries consume rice as staple. The objectives this study were determine variability GI popular improved and traditional varieties find genetic basis GI. A method predict using an vitro system was compared vivo...
Wheat starch contains two classes of associated proteins: proteins which are embedded within the granule and loosely surface proteins. The characterisation major that in described. Gel electrophoresis on basis size resolved these into five bands molecular weights 60, 75, 85, 100 105 kDa. These polypeptides were demonstrated to be by their resistance proteinase K digestion when granules ungelatinised. N-terminal sequences reported. most prominent polypeptide is 60 kDa granule-bound synthase....
The wheat starch 15‐kDa protein (called grain softness or GSP) consists of a major polypeptide and several minor polypeptides. An antiserum raised against GSP was used to screen cDNA library. A family encoding approximately proteins that included heptapeptide sequence previously isolated from protease digests identified. partial in prokaryotic expression system produce fusion which reacted strongly the original anti‐GSP serum. new produced weak reaction extracted seeds. results suggest...
Abstract The starch granules of hexaploid wheat (Triticum aestivum) contain a group three proteins known as SGP-1 (starch granule protein-1) proteins, which have apparent molecular masses 100, 108, and 115 kD. nature role these has not been defined previously. We demonstrate that polypeptides are synthases present in both the soluble fraction at early stages endosperm development, but exclusively bound mid late development. A partial cDNA clone encoding fragment 100-kD protein was obtained...
Abstract Genes and cDNAs for starch-branching enzyme II (SBEII) have been isolated from libraries constructed Aegilops tauschii wheat (Triticum aestivum) endosperm, respectively. One class of genes has termedwSBEII-DA1 encodes the N terminus reported an SBEII endosperm. On basis phylogenetic comparisons with other branching sequences, wSBEII-DA1 is considered to be a member SBEIIa class. ThewSBEII-DA1 gene consists 22 exons 4 21 being identical in length maize (Zea mays) SBEIIb gene, located...
To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis plants, a genomic DNA fragment from Aegilops tauschii was introduced into ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, which endosperm starch is completely replaced by phytoglycogen. A. D genome donor wheat (Triticum aestivum), and effectively included gene for ISA1 (TaISA1) that encoded on genome. In TaISA1-expressing endosperm, phytoglycogen synthesis substantially synthesis, leaving only residual...
Galleria mellonella larvae have been increasingly used in research, including microbial infection studies. They act as suitable preliminary models to study host-pathogen interactions due their advantages, such the ability survive at 37°C mimicking human body temperature, immune system shares similarities with mammalian systems, and short life cycle allowing large-scale Here, we present a protocol for simple rearing maintenance of G. without requiring special instruments specialized training....
Abstract The endosperm of hexaploid wheat (Triticum aestivum[L.]) was shown to contain a high molecular weight starch synthase (SS) analogous the product maize du1 gene, III (SSIII; DU1). cDNA and genomic DNA sequences encoding SSIII were isolated characterized. is 5,346 bp long contains an open reading frame that encodes 1,628-amino acid polypeptide. A putative N-terminal transit peptide, 436-amino C-terminal catalytic domain, central 470-amino SSIII-specific domain containing three regions...
ABSTRACT Granule‐bound starch synthase (GBSS) is the primary enzyme responsible for synthesis of amylose in amyloplasts cereal endosperm cells. Bread wheats, due to their hexaploid genetic system, carry three genes ( wx loci) encoding GBSS. Purification and separation GBSS from more than 200 North American wheats allowed identification genotypes that null alleles at either wx‐A1 wx‐B1 loci. In addition, cultivar Ike carried both alleles. No wx‐D1 nulls were detected. Null found 10% hard...
An important determinant of wheat grain quality is the hardness grain. The trait controlled by a major locus, Ha, on short arm chromosome 5D. Purified starch granules from soft-grained wheats have associated with them 15-kDa polypeptides called softness proteins (GSPs) or "friabilins." Genes that encode one family closely related GSP - GSP-1 genes were mapped using substitution lines to group 5 chromosomes. F2 population segregating for hard and soft alleles at Ha locus near-isogenic...