A5046871752- Congenital gastrointestinal and neural anomalies
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Kruppel-like factors research
- HIV Research and Treatment
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Appendicitis Diagnosis and Management
- Urological Disorders and Treatments
- Cancer-related gene regulation
- HIV/AIDS Research and Interventions
- HIV, Drug Use, Sexual Risk
- Congenital Diaphragmatic Hernia Studies
- Mathematical and Theoretical Epidemiology and Ecology Models
- Immune Cell Function and Interaction
- Allergic Rhinitis and Sensitization
- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Congenital Anomalies and Fetal Surgery
- T-cell and B-cell Immunology
- Intraperitoneal and Appendiceal Malignancies
- Intestinal Malrotation and Obstruction Disorders
- Gastrointestinal motility and disorders
- Nonlinear Differential Equations Analysis
China Medical University
2009-2025
Xian Center for Disease Control and Prevention
2014-2024
IGE Therapeutics (United States)
2022-2024
Southwest University of Science and Technology
2022
Xi'an Jiaotong University
2022
Ministry of Agriculture and Rural Affairs
2021
South China Agricultural University
2021
Banner - University Medical Center Tucson
2020
Herbert Irving Comprehensive Cancer Center
2015
Columbia University Irving Medical Center
2015
Genetic studies have identified numerous sequence-specific transcription factors that control development, yet little is known about their in vivo distribution across animal genomes. We determined the genome-wide occupancy of dorsoventral (DV) determinants Dorsal, Twist, and Snail Drosophila embryo using chromatin immunoprecipitation coupled with microarray analysis (ChIP–chip). The binding these proteins correlate tightly limits enhancers. Our predicts substantially more target genes than...
Protein-protein interactions are known to be essential for specifying the transcriptional activities of homeoproteins.Here we show that representative members Msx and Dlx homeoprotein families form homoand heterodimeric complexes.We demonstrate dimerization by proteins is mediated through their homeodomains residues required this interaction correspond those necessary DNA binding.Unlike most other examples interactions, association does not promote cooperative binding; instead, binding...
Human CtBP attenuates transcriptional activation and tumorigenesis mediated by the adenovirus E1A protein. The sequence motif that interacts with CtBP, Pro-X-Asp-Leu-Ser-X-Lys (P-DLS-K), is present in repression domains of two unrelated short-range repressors Drosophila , Knirps Snail, essential for interaction these proteins (dCtBP). A P-element–induced mutation dCtBP exhibits gene-dosage interactions a null knirps which consistent occurrence Knirps-dCtBP vivo. These observations suggest...
Significance Plants are a primary source of both traditional and modern drugs due to their astounding capability synthesize diverse molecules. The fruit tree loquat in the apple tribe has been long used medicine treat cough, chronic bronchitis, asthma, yet why loquat—but not its relatives—evolved these medicinal properties is unknown. Here, we generate high-quality genomes relative, which separated by recent whole-genome duplication (WGD) around 13.5 27.1 Mya. We revealed post-WGD...
AbstractThis study investigates the transcriptional properties of Msx-1, a murine homeodomain protein which has been proposed to play key role in regulating differentiation and/or proliferation state specific cell populations during embryogenesis. We show, using basal and activated transcription templates, that Msx-1 is potent repressor can function through both TATA-containing TATA-less promoters. Moreover, repression vivo vitro occurs absence DNA-binding sites for homeodomain. Utilizing...
Relatively little is known about the molecular events that specify rostrocaudal axis of neural plate. Here we show a member Distal-less (Dlx) homeobox gene family, Dlx5, one earliest markers for most rostral ectoderm, before formation an overt During late gastrulation Dlx5 expression becomes localized to anterior ridge, which defines boundary plate, and also extends caudolaterally, marking region presumptive crest. Subsequently, expressed in tissues (olfactory epithelium, ventral cephalic...
The precellular Drosophila embryo contains ≈10 well characterized transcriptional repressors. At least half are short-range repressors that must bind within 100 bp of either upstream activators or the core transcription complex to inhibit (or quench) gene expression. two long-range can function over distances 1 kilobase more silence transcription. Previous studies have shown three five interact with a common corepressor protein, dCtBP. In contrast, repressors, Hairy and Dorsal, recruit...
A Dpp activity gradient specifies multiple thresholds of gene expression in the dorsal ectoderm early embryo. Some these depend on a putative repressor, Brinker, which is expressed neurogenic response to maternal Dorsal and signaling. Here we show that Brinker sequence-specific transcriptional repressor. It binds consensus sequence, TGGCGc/tc/t, interacts with Groucho corepressor through conserved sequence motif, FKPY. An optimal binding site contained within an 800-bp enhancer from tolloid...
Previously, we found that the cause of autosomal dominant selective tooth agenesis in one family is a missense mutation resulting an arginine-to-proline substitution homeodomain MSX1. To determine whether phenotype may result from haploinsufficiency or dominant-negative mechanism, have performed biochemical and functional analyses mutant protein Msx1(R31P). We show Msx1(R31P) has perturbed structure reduced thermostability compared with wild-type Msx1. As consequence, activities are severely...
In the mouse embryo and differentiating embryonic stem cells, hematopoietic, endothelial, cardiomyocyte lineages are derived from Flk1+ mesodermal progenitors. Here, we report that surface expression of Podocalyxin (Podxl), a member CD34 family sialomucins, can be used to subdivide cells in embryoid bodies at day 4.75 into populations develop distinct lineages. Definitive hematopoietic potential was restricted Flk1+Podxl+ population, while Flk1-negative Podxl+ population displayed only...
BACKGROUND The human prostate tumor suppressor NKX3.1 mediates the DNA repair response and interacts with androgen receptor to assure faithful completion of transcription thereby protecting against TMPRSS2-ERG gene fusion. To determine directly effect Nkx3.1 in vivo we studied prostates mice targeted deletion Nkx3.1. METHODS Using both drug-induced damage γ-irradiation, assayed expression γ-histone 2AX at time points up 24 hr after induction damage. RESULTS We demonstrated that influenced...
Members of the Mix/Bix family paired class homeobox genes play important roles in development vertebrate mesoderm and endoderm. The single member identified mouse, Mix-like 1 (Mixl1), is required for mesendoderm patterning during gastrulation promotes formation hematopoiesis embryonic stem cell (ESC)-derived embryoid bodies. Despite its crucial functions transcriptional activity targets Mixl1 have not been well described. To investigate molecular mechanisms Mixl1-mediated regulation, we...
Men who have sex with men (MSM) continue to be disproportionately impacted by human immunodeficiency virus (HIV) and syphilis in China. Little is known about HIV infections among MSM Xi'an, a developing city Northwest A cross-sectional study recruitment via snowball sampling was conducted collect infection status risk factors Xi'an between April 2013 December 2016. Among the 5000 participants, mean age 29.0 years (SD 7.7) prevalence of HIV, syphilis, coinfection 6.5%, 2.2%, 0.4%,...
In children less than 3 years of age, there is little experience in the nonoperative management appendiceal phlegmon or abscess (APA), especially APA with an appendicolith. The purposes this study were to evaluate effects appendicolith and success rate for these young children. Children younger age who underwent attempted initial treatment between January 2008 December 2016 reviewed. Based on presence absence admission ultrasonography examination computed tomography scan, divided into two...