A5100357262- Congenital gastrointestinal and neural anomalies
- Congenital Anomalies and Fetal Surgery
- Pelvic floor disorders treatments
- Gastrointestinal motility and disorders
- Anorectal Disease Treatments and Outcomes
- Urological Disorders and Treatments
- Hernia repair and management
- Spinal Dysraphism and Malformations
- Congenital Diaphragmatic Hernia Studies
- Intestinal Malrotation and Obstruction Disorders
- Gastrointestinal disorders and treatments
- Esophageal and GI Pathology
- Diverticular Disease and Complications
- Circular RNAs in diseases
- Mesenchymal stem cell research
- Digestive system and related health
- RNA modifications and cancer
- Neurogenetic and Muscular Disorders Research
- Fetal and Pediatric Neurological Disorders
- Pediatric Hepatobiliary Diseases and Treatments
- Pregnancy and preeclampsia studies
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Congenital heart defects research
- Tumors and Oncological Cases
Shandong University
2025
China Medical University
2015-2024
Dazhou Central Hospital
2024
Second Affiliated Hospital of Zhejiang University
2024
Wenzhou Medical University
2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2018
Chongqing Medical University
2018
Shenyang The Fourth Hospital of People
2010
Abstract To identify candidate serum molecule biomarkers for the non-invasive early prenatal diagnosis of neural tube defects (NTDs), we employed an iTRAQ-based quantitative proteomic approach to analyze changes in samples from embryonic day (E) 11 and E13 pregnant rats with spina bifida aperta (SBA) induced by all-trans retinoic acid. Among 390 proteins identified, 40 at E11 26 displayed significant differential expression SBA groups. We confirmed 5 ELISA. observed space-time proprotein...
Neural tube defects (NTDs) are complex congenital malformations resulting from incomplete neurulation in embryo. Despite surgical repair of the defect, most patients who survive with NTDs have a multiple system handicap due to neuron deficiency defective spinal cord. In this study, we successfully devised prenatal approach and transplanted mesenchymal stem cells (MSCs) foetal rat column treat retinoic acid induced rat. Transplanted MSCs survived, grew expressed markers neurons, glia...
Spina bifida aperta are complex congenital malformations resulting from failure of fusion in the spinal neural tube during embryogenesis. Despite surgical repair defect, most patients who survive with spina have a multiple system handicap due to neuron deficiency defective cord. Tissue engineering has emerged as novel treatment for replacement lost tissue. This study evaluated prenatal approach transplanting chitosan–gelatin scaffold seeded bone marrow mesenchymal stem cells (BMSCs) healing...
Abstract Spina bifida is a complex congenital malformation resulting from failure of fusion in the spinal neural tube during embryogenesis. However, cellular mechanism underlying spina not fully understood. Here, we investigated cell apoptosis whole embryos and proliferation progenitor cells neurulation all‐trans retinoic acid (atRA)‐induced fetal rats. Cell was assessed by TUNEL assay on whole‐mount serially sectioned samples rat with bifida. lumbo‐sacral staining for mitotic marker Ki67...
Abstract Neural tube defects (NTDs) lead to prenatal mortality and lifelong morbidity. Currently, surgical closure of NTD lesions results in limited functional recovery. We previously suggested that nerve regeneration was critical for therapy. Here, we report transamniotic bone marrow-derived mesenchymal stem cell (BMSC) therapy NTDs during early development may achieve beneficial In our ex vivo rat embryonic model, BMSCs injected into the amniotic cavity spontaneously migrated defective...
Abstract BACKGROUND: Defects of the pelvic nerve innervation levator ani muscle are associated with poor postoperative anorectal function in patients malformation (ARM). We have previously shown deficient development motoneurons innervating rats ARM. In this study we investigate whether there is a deficiency sensory neurons that innervate METHODS: ARM was induced by ethylenethiourea (ETU) fetal rats. Retrograde tracer fluorogold (FG) injected into muscle. Serial transverse sections...
Neural tube defects (NTDs) result in prenatal mortality and lifelong morbidity, available treatments have limited efficacy. We previously suggested that bone marrow-derived mesenchymal stem cell (BMSC) transplantation could treat neuron deficiency NTD rats; however, BMSC-based therapy is by the low survival rate of BMSCs when used to severe NTDs. Herein, a new using combined BMSC small interfering RNA collapsin response mediator protein 4 (CRMP4 siRNA), which was identified as novel...
The aims of this study were to identify the mutation gene a Chinese family with anorectal malformation (ARM) associated split hand-foot and determine spatiotemporal expression mutated during hindgut anorectum development in human embryos.A intrafamilial clinically variable manifestation was analyzed primers designed for exons 3-14 P63, DLX5, DLX6, DAC, HOXD13 as candidate genes direct sequence analysis performed. Immunohistochemical embryos 4th-10th weeks performed.Affected individuals found...
BACKGROUND In previous studies, we found that the deficiency of sensory and motor neurons was a primary defect associated with spinal malformation. Upon prenatal treatment spina bifida through in utero stem cell transplantation retinoic acid‐induced rat model, mesenchymal (MSCs) survived, migrated, differentiated into cells neural lineage. present study, investigated whether transplanted MSCs had potential to differentiate or protect defective cord. METHODS Pregnant rats treated acid on...
The discovery of N6-methyladenosine (m
Anorectal malformations (ARMs) are congenital diseases that lead to postoperative fecal incontinence, constipation, and soiling, despite improvements in surgery; however, their pathological mechanisms remain unclear. Here, we report the role of microRNA-141-3p maintaining homeostasis between apoptosis autophagy lumbosacral defecation center fetal rats with ARMs. Elevated expression inhibited YIN-YANG-1 by binding its 3' UTR, repressed triggered simultaneously. Then, adenylate cyclase 3 was...
Hepatoblastoma is one of the malignant liver tumors in children.However, genetic mechanisms underpinning initiation hepatoblastoma remain largely unclear.The previous study showed that lin-28 homolog B (LIN28B) might play a role development hepatoblastoma.To detect association between LIN28B gene polymorphisms and risk Chinese children, we conducted five-center case-control 275 patients 1018 cancer-free controls.Four potentially functional were genotyped using Taqman method.Odds ratios (ORs)...
Spina bifida aperta (SBA) is one of the most common neural tube defects. Neural injury in SBA occurs two stages involving failed closure and progressive degeneration through contact with amniotic fluid. We previously suggested that intra-amniotic bone marrow-derived mesenchymal stem cell (BMSC) therapy for fetal rat could achieve beneficial functional recovery lesion-specific differentiation. The aim this study to examine whether fluid microenvironment can be improved by BMSC...
Heterotopic pancreas (HP) is a congenital anomaly defined as pancreatic tissue that has no contact with the orthotopic and its own duct system vascular supply. The most common locations of HP are upper gastrointestinal tract, specifically, stomach, duodenum, proximal jejunum. Involvement mesentery rare. Here, we describe rare case mesenteric heterotopic (MHP) in 12-year-old girl who presented acute abdomen. patient underwent emergency laparotomy, mass adjacent small bowel were resected....