A5081185604- Neurogenesis and neuroplasticity mechanisms
- Neurobiology and Insect Physiology Research
- Olfactory and Sensory Function Studies
- Insect Pheromone Research and Control
- Coral and Marine Ecosystems Studies
- Reproductive System and Pregnancy
- Axon Guidance and Neuronal Signaling
- Crustacean biology and ecology
- Multiple Sclerosis Research Studies
- TGF-β signaling in diseases
- Animal Behavior and Reproduction
- Neuroinflammation and Neurodegeneration Mechanisms
- Spinal Dysraphism and Malformations
- Insect and Arachnid Ecology and Behavior
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Biochemical Analysis and Sensing Techniques
- Mesenchymal stem cell research
- Advanced Neuroimaging Techniques and Applications
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
James Cook University
2024
MainStream Aquaculture (Australia)
2024
The University of Melbourne
2007-2019
Florey Institute of Neuroscience and Mental Health
2006-2015
Georgia State University
1997-2004
Whitney Museum of American Art
1998
University of Florida
1998
University of North Carolina at Chapel Hill
1997
University of North Carolina Wilmington
1997
The critical role of oligodendrocytes in producing and maintaining myelin that supports rapid axonal conduction CNS neurons is well established. More recently, additional roles for have been posited, including provision trophic factors metabolic support neurons. To investigate the functional consequences oligodendrocyte loss, we generated a transgenic mouse model conditional ablation. In this model, are rendered selectively sensitive to exogenously administered diphtheria toxin (DT) by...
The TAM family of receptor protein tyrosine kinases comprises three known members, namely Tyro3, Axl, and Mer. These receptors are widely expressed in the nervous system, including by oligodendrocytes, cell type responsible for myelinating CNS. We examined potential role their principle cognate ligand, Gas6 (growth arrest gene 6), modulating phenotype cuprizone model demyelination. found that expression profiles Axl , Mer mRNA were increased corpus callosum a temporal profile correlating...
Remyelination of the CNS involves regeneration mature oligodendrocytes by endogenous oligodendrocyte progenitor cells (OPCs). Previous studies have shown that bone morphogenic proteins (BMPs) inhibit production in healthy CNS. However, there is currently no information on influence BMP signaling vivo within demyelinated lesions brain or subsequent remyelination. Here, we determine a role for modulating oligodendrogliogenesis and remyelination following cuprizone-induced demyelination. We...
Oligodendrocytes are responsible for producing and maintaining myelin throughout the CNS. One of pathological features observed following traumatic brain injury (TBI) is progressive demyelination degeneration axons within white matter tracts. While effect TBI on axonal health has been well documented, there limited information regarding response oligodendrocytes these areas. The aim this study was to characterize both mature immature proliferative oligodendrocyte lineage cells across a 3...
Differentiation of oligodendrocyte progenitor cells (OPCs) into mature oligodendrocytes is regulated by the interplay between extrinsic signals and intrinsic epigenetic determinants. In this study, we analyze effect that extracellular ligands sonic hedgehog (Shh) bone morphogenetic protein 4 (BMP4), have on histone acetylation gene expression in cultured OPCs. Shh treatment favored progression toward decreasing inducing peripheral chromatin condensation. BMP4 treatment, contrast, inhibited...
A complex nervous system enables spiny lobsters to have a rich behavioural repertoire. The present paper discusses the ways in which sensory systems of Caribbean lobster, Panulirus argus, particularly its chemosensory systems, are involved feeding behaviour. It addresses neural mechanisms three aspects their food-finding ability: detection, identification, and discrimination natural food odours; effect learning on responses by orient odours from distance under flow conditions. demonstrates...
Abstract Leukemia inhibitory factor (LIF) receptor signaling limits the severity of inflammatory demyelination in experimental autoimmune encephalomyelitis, a T‐cell dependent animal model multiple sclerosis (MS) [Butzkueven et al. ( 2002 ) Nat Med 8:613–619]. To identify whether LIF exerts direct effects within central nervous system to limit demyelination, we have studied influence upon phenotype mice challenged with cuprizone, copper chelator, which produces toxic oligodendrocytopathy. We...
Abstract Purpose To characterize and compare histological MRI‐based changes within the corpus callosum (CC) in cuprizone mouse model of multiple sclerosis (MS). Materials Methods A total 12 C57/BL6 mice were fed from eight weeks age for four weeks. One cohort six two control scanned with a T2‐weighted (T2W) sequence. The other using dual‐echo sequence T2‐mapping diffusion‐weighted orthogonal diffusion encoding directions to calculate water diffusivities parallel perpendicular CC fiber...
The adult subventricular zone (SVZ) is a potential source of precursor cells to replace neural lost during demyelination. To better understand the molecular events that regulate cell responsiveness in this context we undertook microarray and quantitative PCR based analysis genes expressed within SVZ cuprizone-induced We identified an up-regulation encoding bone morphogenic protein 4 (BMP4) its receptors. Immunohistochemistry confirmed increase BMP4 levels also showed phosphorylated SMAD...
Abstract Astrocytes are a target for regenerative neurobiology because in brain injury their phenotype arbitrates integrity, neuronal death and subsequent repair reconstruction. We explored the ability of 3D scaffolds to direct astrocytes into phenotypes with potential support survival. Poly‐ε‐caprolactone were electrospun random aligned fibre orientations on which murine sub‐cultured analysed at 4 12 DIV. survived, proliferated migrated adopting morphologies, mimicking vivo stellated...
Enhancement of oligodendrocyte survival through activation leukemia inhibitory factor receptor (LIFR) signaling is a candidate therapeutic strategy for demyelinating disease. However, in other cell types, LIFR under tight negative regulation by the intracellular protein suppressor cytokine 3 (SOCS3). We, therefore, postulated that deletion SOCS3 gene oligodendrocytes would promote beneficial effects limiting demyelination. By studying wild-type and LIF-knockout mice, we established...
Inhibitors of Rho kinase (ROCK) have potential for management neurological disorders by inhibition glial scarring. Since astrocytes play key roles in brain physiology and pathology, we determined changes the astrocytic transcriptome produced ROCK inhibitor Fasudil to obtain mechanistic insights into its beneficial action during injury. Cultured murine were treated with (100 µM) morphological analyses revealed rapid stellation 1 h time-dependent (2-24 h) dissipation F-actin-labelled stress...
Olfactory receptor neurons (ORNs) of crustaceans are housed in aesthetasc sensilla that located on the lateral flagellum antennule. We used young adult spiny lobsters to examine turnover aesthetascs and functional maturation their ORNs after molting. The proliferation zone for new is proximal part aesthetasc-bearing region progressively moves along a distoproximal axis. Older lost distal region. As result, an may be shed three six molts it differentiates. Taurine-like immunoreactivity...
Multiple sclerosis is an autoimmune disease of the CNS resulting in degeneration myelin sheaths and loss oligodendrocytes, which means that protection electrical insulation axons rapid signal propagation are impaired, leading to axonal damage permanent disabilities. Partial replacement lost oligodendrocytes remyelination can occur as a result activation recruitment resident oligodendroglial precursor cells. However, overall capacity remains inefficient because cells often fail generate new...
The lateral flagellum of the antennule spiny lobster Panulirus argus houses more than 1,000 morphologically similar olfactory sensilla, called aesthetascs. By using a high-resolution activity labeling technique that depends on entry agmatine into receptor neurons (ORNs) through cation channels during odor stimulation, we examined distribution different functional types ORNs within and across mature A significant number in aesthetascs are labeled with stimulation by single odorants, including...
Abstract Olfactory systems undergo continuous growth and turnover in many animals. Many decapod crustaceans, such as lobsters crayfish, have indeterminate growth, these animals, of both peripheral central components the olfactory system occurs continuously throughout life. In this study, we examine dynamics receptor neuron (ORN) proliferation antennule Caribbean spiny lobster, Panulirus argus, using vivo incorporation cell marker BrdU. We show that addition ORNs a “proximal zone” (PPZ),...
Abstract The antennules of decapod crustaceans are covered with thousands chemosensilla that mediate odor discrimination and orientation behaviors. Most studies on chemoreception in decapods have focused the prominent aesthetasc sensilla. However, previous behavioral lobsters following selective sensillar ablation revealed input from nonaesthetasc antennular is sufficient for many odor‐mediated Our earlier examination setal types Caribbean spiny lobster Panulirus argus three chemosensilla....
Blocking inhibitory factors within CNS demyelinating lesions is regarded as a promising strategy to promote remyelination. Bone morphogenetic protein 4 (BMP4) an factor present in lesions. Noggin, endogenous antagonist BMP, has previously been shown increase the number of oligodendrocytes and remyelination vivo. However, it remains unclear how BMP4 signaling inhibits Here we investigated downstream pathway that mediates effect exerts upon through pharmacological transgenic approaches. Using...
Abstract A diversity of sensilla has been described in crustaceans, both across species and within a given species. However, few homologous setal types have identified crustaceans. In this study we examined setae with features the hooded sensillum, which is class bimodal chemomechanosensilla first on antennules Caribbean spiny lobster Panulirus argus . We 13 representing seven families malacostracan most body surfaces P. , compared sensillar morphology from different regions to identify...
In order to further investigate the molecular mechanisms that regulate oligodendrocyte (OC) survival, we utilized microarrays characterize changes in OC gene expression after exposure cytokines neurotrophin3, insulin, or leukemia inhibitory factor (LIF) vitro. We identified and validated induction secretion of neuropeptide galanin OCs, specifically response LIF. next established is an survival showed autocrine paracrine mediates LIF-induced also revealed up-regulated OCs cuprizone model...