A5013425481- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- Neonatal Respiratory Health Research
- Mesenchymal stem cell research
- Peripheral Neuropathies and Disorders
- Myasthenia Gravis and Thymoma
- Venomous Animal Envenomation and Studies
- Nicotinic Acetylcholine Receptors Study
- Fibroblast Growth Factor Research
- Photochromic and Fluorescence Chemistry
- Transcranial Magnetic Stimulation Studies
- Vagus Nerve Stimulation Research
- Receptor Mechanisms and Signaling
- Immunotherapy and Immune Responses
- Calcium signaling and nucleotide metabolism
- Pancreatic function and diabetes
- Organ and Tissue Transplantation Research
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Neuroblastoma Research and Treatments
- Transplantation: Methods and Outcomes
- Marine Toxins and Detection Methods
- Genetics and Neurodevelopmental Disorders
- Neuroscience of respiration and sleep
- Neurological disorders and treatments
Universidade do Porto
2012-2024
Stockholm University
2008
Summary Intrahepatic immune cells (IHIC) are known to play central roles in immunological responses mediated by the liver, and isolation phenotypic characterization of these is therefore considerable importance. In present investigation, we developed a simple procedure for mechanical disruption mouse liver that allows efficient IHIC. These compared with corresponding purified from after enzymatic digestion different concentrations collagenase DNase. The yielded viable IHIC considerably...
Polymorphisms of the P2X7 receptor have been associated with increased risk fractures in postmenopausal women. Although both osteoblasts and osteoclasts express receptors, their function osteogenesis remains controversial. Here, we investigated role on osteogenic differentiation mineralization bone marrow mesenchymal stem cell (BMSC) cultures from women (age 71 ± 3 yr, n=18). We focused mechanisms related to intracellular [Ca2+]i oscillations plasma membrane-dynamics. ATP, agonist BzATP (100...
Abstract This study aimed at investigating the expression and function of uracil nucleotide‐sensitive receptors (P2Y 2 , P2Y 4 6 ) on osteogenic differentiation human bone marrow stromal cells (BMSCs) in culture. Bone specimens were obtained from postmenopausal female patients (68 ± 5 years old, n = 18) undergoing total hip arthroplasty. UTP UDP (100 µM) facilitated measured as increases alkaline phosphatase (ALP) activity, without affecting cell proliferation. Uracil nucleotides...
In healthy motor endplates, tetanic depression is overcome by tonic adenosine A(2A) -receptor-mediated facilitation of transmitter release. The receptor operates a coordinated shift from fast-desensitizing Ca(v) 2.1 (P/Q) calcium influx to long-lasting Ca(V) 1 (L) channels on nerve terminals. This study aimed at investigating whether receptors-operated Ca(2+) via (L)-type contribute sustain acetylcholine release evoked 50 Hz-bursts in toxin-induced Myasthenia gravis (TIMG) rats. contrast...
Acetylcholine (ACh) spillover from motor endplates occurs after neuronal firing bursts being potentiated by cholinesterase inhibitors (e.g., neostigmine). Nicotinic α7 receptors (α7nAChR) on perisynaptic Schwann cells (PSCs) can control ACh unknown mechanisms. We hypothesized that adenosine might be the gliotransmitter underlying PSCs-nerve terminal communication. Rat isolated hemidiaphragm preparations were used to measure (1) outflow of [3 H]ACh, (2) real-time transmitter exocytosis...
Abstract Background Endogenously released adenine and uracil nucleotides favour the osteogenic commitment of bone marrow-derived mesenchymal stromal cells (BM-MSCs) through activation ATP-sensitive P2X7 UDP-sensitive P2Y 6 receptors. Yet, these have their potential compromised in post-menopausal (Pm) women due to overexpression nucleotide metabolizing enzymes, namely NTPDase3. This prompted us investigate whether NTPDase3 gene silencing or inhibition its enzymatic activity could rehabilitate...
Mechanical stimulation (MS) significantly increases the release of adenine and uracil nucleotides from bone marrow-derived mesenchymal stem cells (BM-MSCs) undergoing osteogenic differentiation. Released acting via ionotropic P2X7 metabotropic P2Y
<b><i>Background/Aims:</i></b> In this study, we evaluated the functional impact of facilitatory presynaptic adenosine A<sub>2A</sub> and muscarinic M<sub>1</sub> receptors in recovery neuromuscular tetanic depression caused by blockage high-affinity choline transporter (HChT) hemicholinium-3 (HC-3), a condition that mimics myasthenia-like condition. <b><i>Methods:</i></b> Rat diaphragm preparations were indirectly...