A5051425071- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Biosimilars and Bioanalytical Methods
- Immune Response and Inflammation
- Computational Drug Discovery Methods
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- interferon and immune responses
- Ultrasound and Hyperthermia Applications
- Inflammatory mediators and NSAID effects
- Adipokines, Inflammation, and Metabolic Diseases
- RNA Interference and Gene Delivery
- Inflammasome and immune disorders
- Lipid metabolism and biosynthesis
- Microbial Inactivation Methods
- Cancer, Stress, Anesthesia, and Immune Response
- Adipose Tissue and Metabolism
- Microfluidic and Bio-sensing Technologies
- Malaria Research and Control
Radboud University Nijmegen
2018-2023
Radboud University Medical Center
2018-2023
Radboud Institute for Molecular Life Sciences
2018-2023
University Medical Center
2018
Introduction In situ tumor ablation releases a unique repertoire of antigens from heterogeneous population cells. High-intensity focused ultrasound (HIFU) is completely noninvasive therapy that can be used to ablate tumors either by heating (thermal (T)-HIFU) or mechanical disruption (mechanical (M)-HIFU). How different HIFU techniques compare with respect their antigen release profile, activation responder T cells, and ability synergize immune stimuli remains elucidated. Methods results...
The recent decline in global malaria burden has stimulated efforts toward Plasmodium falciparum elimination. Understanding the biology of transmission stages may provide opportunities to reduce or prevent onward mosquitoes. Immature P. stages, termed I IV gametocytes, sequester human bone marrow before release into circulation as mature stage V gametocytes. This process likely involves interactions between host receptors and potentially immunogenic adhesins on infected red blood cell (iRBC)...
Background Tumor ablation techniques, like cryoablation, are successfully used in the clinic to treat tumors. The tumor debris remaining situ after is a major antigen depot, including neoantigens, which presented by dendritic cells (DCs) draining lymph nodes induce tumor-specific CD8 + T cells. We have previously shown that co-administration of adjuvants essential evoke strong vivo antitumor immunity and induction long-term memory. However, most effectively combine with remains unclear....
Abstract Cytotoxic T lymphocytes (CTL) mediate cytotoxicity toward tumor cells by multistep cell–cell interactions. However, the microenvironment can metabolically perturb local CTL effector function. activity is typically studied in two-dimensional (2D) liquid coculture, which limited recapitulating mechanisms and efficacy of response. We here developed a microscopy-based, automated three-dimensional (3D) interface coculture model suitable for medium-throughput screening to delineate steps...
Immunotherapy is now considered as the new pillar in treatment of cancer patients. Dendritic cells (DCs) play an essential role stimulating anti-tumor immune responses, they are capable cross-presenting exogenous tumor antigens MHCI complexes to activate naïve CD8+ T cells. Analgesics, like non-steroid anti-inflammatory drugs (NSAIDs), frequently given patients help relieve pain, however little known about their impact on DC function.Here, we investigated effect NSAIDs diclofenac, ibuprofen...
<div>Abstract<p>Cytotoxic T lymphocytes (CTL) mediate cytotoxicity toward tumor cells by multistep cell–cell interactions. However, the microenvironment can metabolically perturb local CTL effector function. activity is typically studied in two-dimensional (2D) liquid coculture, which limited recapitulating mechanisms and efficacy of response. We here developed a microscopy-based, automated three-dimensional (3D) interface coculture model suitable for medium-throughput screening...
<p>Supplemental Figures and Legends for Supplemental S1-S8, Movie Movies 1-7</p>
<p>Single CTL-associated MEC1/OVA Ca2+ event followed by target cell death in control culture (DMSO) and lack of events despite stable CTL-target interactions the presence BTP2. Arrowhead highlights event. Time stamp, h:min. Fire LUT, intensity. Scale bar, 10 µm.</p>
<p>Human CTL engagement with an αCD3-coated surface in the absence (DMSO) or presence of BTP2. Morgenstemning LUT, Fura-2 340/380 ratio. Time stamp, min:sec (0 min denoting time-point contact initiation). Scale bar, 10 µm.</p>
<p>OT-I CTL conjugation with MEC1/OVA cells in the absence (DMSO) or presence of BTP2. Time stamp, min:sec. Fire LUT, Fura-2 340/380-ratio. Scale bar, 10 µm.</p>
<p>OT-I CTL engaging with a MEC1/OVA target cell followed by rounding and death in the absence (DMSO) or presence of BTP2. Time stamp, h:min. Scale bar, 10 µm.</p>
<p>OT-I CTL conjugation with B16F10/OVA cells in the absence (left) or presence (right) of 100 µM H2O2. Morgenstemning LUT, Fura-2 340/380-ratio. Time stamp, min:sec (0 min denoting timepoint contact initiation). Scale bar, 10 µm.</p>
<p>OT-I CTL engagement with H2B-GFP-labeled MEC1/OVA cells followed by target cell death. The migration tracks and position of individual CTLs (numbers) are highlighted. Arrowheads highlight different phases death, including membrane blebbing, chromatin condensation, nuclear fragmentation fading GFP fluorescence (green color). Time stamp, h:min. Scale bar, 20 µm.</p>
<p>OT-I CTLs engaging with and detaching from B16F10/OVA cells in 2D matrix-free or 3D interface co-culture. Note the frequent instable interactions co-culture but not culture. Time stamp, h:min:sec. Scale bar, 10 µm.</p>
<div>Abstract<p>Cytotoxic T lymphocytes (CTL) mediate cytotoxicity toward tumor cells by multistep cell–cell interactions. However, the microenvironment can metabolically perturb local CTL effector function. activity is typically studied in two-dimensional (2D) liquid coculture, which limited recapitulating mechanisms and efficacy of response. We here developed a microscopy-based, automated three-dimensional (3D) interface coculture model suitable for medium-throughput screening...
<p>OT-I CTL conjugation with MEC1/OVA cells in the absence (DMSO) or presence of BTP2. Time stamp, min:sec. Fire LUT, Fura-2 340/380-ratio. Scale bar, 10 µm.</p>
<p>Supplemental Figures and Legends for Supplemental S1-S8, Movie Movies 1-7</p>
<p>OT-I CTL engaging with a MEC1/OVA target cell followed by rounding and death in the absence (DMSO) or presence of BTP2. Time stamp, h:min. Scale bar, 10 µm.</p>
<p>OT-I CTLs engaging with and detaching from B16F10/OVA cells in 2D matrix-free or 3D interface co-culture. Note the frequent instable interactions co-culture but not culture. Time stamp, h:min:sec. Scale bar, 10 µm.</p>
<p>Human CTL engagement with an αCD3-coated surface in the absence (DMSO) or presence of BTP2. Morgenstemning LUT, Fura-2 340/380 ratio. Time stamp, min:sec (0 min denoting time-point contact initiation). Scale bar, 10 µm.</p>
<p>OT-I CTL conjugation with B16F10/OVA cells in the absence (left) or presence (right) of 100 µM H2O2. Morgenstemning LUT, Fura-2 340/380-ratio. Time stamp, min:sec (0 min denoting timepoint contact initiation). Scale bar, 10 µm.</p>
<p>Single CTL-associated MEC1/OVA Ca2+ event followed by target cell death in control culture (DMSO) and lack of events despite stable CTL-target interactions the presence BTP2. Arrowhead highlights event. Time stamp, h:min. Fire LUT, intensity. Scale bar, 10 µm.</p>