A5071250446- Salmonella and Campylobacter epidemiology
- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Herpesvirus Infections and Treatments
- Aquaculture disease management and microbiota
- SARS-CoV-2 and COVID-19 Research
- Food Safety and Hygiene
- Identification and Quantification in Food
- Animal Nutrition and Physiology
- Microbial bioremediation and biosurfactants
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Probiotics and Fermented Foods
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Reproductive System and Pregnancy
- Pineapple and bromelain studies
- COVID-19 and Mental Health
- Vibrio bacteria research studies
- Zoonotic diseases and public health
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- Microbial infections and disease research
- Microbial Community Ecology and Physiology
Universidade Estadual de Santa Cruz
2015-2025
Universidade Federal de Campina Grande
2024
Universidade Federal Fluminense
2000-2017
Southwest Bahia State University
2017
This study aimed to identify Leishmania species in small non-flying mammals captured semi-deciduous forest fragments of the Atlantic Forest and pastures Southwest region Bahia state, Northeast Brazil. A total 445 animals belonging 11 different were captured, majority being rodents (75.7%; 337), followed by marsupials (24.2%; 108), most prevalent Cerradomys vivoi, Calomys expulsus, Necromys Lasiurus, Marmosops incanus. Liver, spleen, kidney, heart, lung collected for subsequent molecular...
A tripanossomíase bovina é uma doença causada pelo hemoparasita Trypanosoma vivax, sendo transmitida, principalmente, por insetos hematófagos, mas também via iatrogênica, responsável perdas econômicas significativas. Este estudo teve o objetivo de relatar a ocorrência T. vivax em fazenda produtora leite, localizada no munícipio Itapé, região sul da Bahia, Brasil. Trata-se um descritivo caráter narrativo reflexivo. No início, os animais apresentaram sinais clínicos compatíveis com fase aguda...
An 11-year-old male mixed-breed cat, with exclusively indoor life, presented 3 cough episodes after the owners tested positive by RT-PCR for SARS-CoV-2. The house is inhabited 5 people (3 adults and 2 children), of have shown mild symptoms associated throat discomfort. cat was vaccinated, had no history any previous disease, negative feline coronavirus (FCoV), immunodeficiency virus (FIV) leukaemia (FeLV). Rectal sample collected from SARS-CoV-2 RT-PCR. Viral genome sequences recovered human...
The sharp increase of COVID-19 cases in late 2020 has made Brazil the new epicenter ongoing SARS-CoV-2 pandemic. novel viral lineages P.1 (Variant Concern Gamma) and P.2, respectively identified Brazilian states Amazonas Rio de Janeiro, have been associated with potentially higher transmission rates antibody neutralization escape. In this study, we performed whole-genome sequencing 185 samples isolated from three out five regions, including (North region), Grande do Norte, Paraíba Bahia...
The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk naturally infected sheep.In order accomplish this, 275 lactating ewes were used; these bred extensively 17 estates distributed across nine cities. Polymerase chain reaction amplification used detect T. DNA samples, and indirect immunofluorescence test employed for detection anti-T.gondii IgG antibodies sera, with a cut-off value 1:64.It possible presence parasite 6.5% (18/275) studied...
teria, Planctomycetes, Bacteroidetes, and Firmicutes).Forty percent of the sequences could not be classified into these phyla, demonstrating genetic diversity this microbial community.Only eight isolates had similar to known 16S rRNA cultivable organisms or environmental therefore identified genus level.This method DNA extraction is a useful tool for analysis bacteria responsible petroleum degradation in contaminated environments.
Quantification of Salmonella in asymptomatic carrier animals can be used to assess microbial risk and monitor the level contamination domestic for food production. We examined sensitivity, specificity accuracy real-time qPCR, without pre-enrichment or selective enrichment stages, quantification S. enterica serovar Enteritidis resistant mice, as a model animal. The results were compared with those obtained by traditional bacteriological culture methods, gold standard test. Two hundred...
This study genetically Toxoplasma gondii isolates obtained from pigs intended for human consumption in northeastern Brazil; multilocus PCR-RFLP and sequencing techniques were utilized. Bioassays conducted using the brain tongue of 20 pig heads purchased at butcher shops city Ilheus, Bahia, Brazil. Overall, 11 T. designated TgPgBr06-16 identified. Application with seven molecular markers (SAG1, SAG2, SAG3, BTUB, C22-8, PK1 Apico) identified six different genotypes. Isolates TgPgBr 06, 08, 11,...
Abstract The sharp increase of COVID-19 cases in late 2020 has made Brazil the new epicenter ongoing SARS-CoV-2 pandemic. Novel lineages P.1 and P.2, first identified respectively Manaus Rio de Janeiro, have been associated with potentially higher transmission rates antibody neutralization escape. In this study, we performed a whole-genome sequencing 185 samples isolated from three out five Brazilian regions, including Amazonas (North region), Grande do Norte, Paraíba Bahia (Northeast...
The aim of this study was to detect evidence Toxoplasma gondii using polymerase chain reaction (PCR)-based techniques in oysters (Crassostrea rhizophorae) obtained from the southern coastal region Bahia, Brazil. A total 624 were collected, and gills digestive glands dissected. Each tissue sample separated into pools containing tissues (of same type) three animals, leading a 416 experimental samples for analysis (208 each glands). Molecular PCR-based detection T. AF 146527 repetitive fragment...
COVID-19 is a lethal disease caused by the pandemic SARS-CoV-2, which continues to be public health threat. principally respiratory and often associated with sputum retention cytokine storm, for there are limited therapeutic options. In this regard, we evaluated use of BromAc®, combination Bromelain Acetylcysteine (NAC). Both drugs present mucolytic effect have been studied treat COVID-19. Therefore, sought examine anti-inflammatory BromAc® in tracheal aspirate samples from critically ill...
Abstract Practical methodologies that include food safety and hygiene education in pedagogical activities are strategies to prevent foodborne diseases (FBDs). Thus, the aim of this study was investigate knowledge 7th‐grade middle school students regarding microbiology safety, apply workshop‐based educational focus on scientific literacy. The (144) were initially evaluated using a Likert‐scale questionnaire (pre‐intervention, Q0) with ten objective questions safety. Once evaluated,...
Quantitative real-time polymerase chain reactions (qPCRs) of the most prevalent bacteria causing foodborne diseases worldwide, such as Salmonella spp., Escherichia coli, and Staphylococcus aureus, can be an important tool for quantitative microbial risk assessment, which requires numerical data to determine level contamination at a specific stage food production. However, qPCR assays described in literature these pathogens are qualitative; their objective is pathogen detection not...
The genotyping of 25 isolates Toxoplasma gondii from free-range chickens in the state Bahia, Brazil, was performed by PCR-restriction fragment length polymorphism using 11 genetic markers: SAG1, 5′ 3′SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3. analysis revealed 8 genotypes, 3 which had not been previously reported. Four genotypes were represented single isolates, whereas other 2 or more isolates. Five showed mixed infections, them identical. None clonal types...
Wild animals have an ecological function and can serve as sentinels to identify infectious agents indicators of environmental health. Among the zoonotic pathogens, Salmonella spp. deserve special attention due their high worldwide prevalence ubiquity hosts. With aim investigating presence in wild birds from Atlantic Forest southern Bahia, Brazil, we collected 114 fecal samples (14 families) between 2016 2017. Fecal were by means cloacal swab subjected microbiological culture isolate serotype...
Summary Species of tegu ( Tupinambis ) are the largest lizards in South America. Large numbers these hunted; there is a vigorous trade their skins and meat consumed by rural native peoples. The animals also bred captivity, an economic activity for populations which can help animals’ conservation. Faecal samples from 30 captive‐born tegus were analysed presence Salmonella two separate samplings. In first analysis, 26 (87%) yielded enterica 23% Rubislaw serotype; 20% Carrau Agona serotypes; 7%...
The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), challenged public health systems worldwide. Individuals in low-income countries/regions are still at individual and community risk concerning inequality, sanitation, economic conditions. Besides, during transmission municipalities communities countryside less developed regions kept viral spread required structured strengthened clinical laboratory surveillance. Here, we present an observational,...
Microbiological evaluation is one of the most important parameters for analyzing viability an oyster farming system, which addresses public health and ecological concerns.Here, microbiological quality Crassostrea rhizophorae cultivated in a monitored environment from natural beds Bahia, northeastern Brazil, was determined.Bacterial diversity oysters measured by polymerase chain reaction-denaturing gradient gel electrophoresis.Sequence analysis revealed that bacterial species showed...
The goal of this study was to assess the presence and surfactant potential naturally occurring microbes from a tropical soil with petrochemical contamination. Microorganisms in sample Brazilian landfarm were isolated grown on petroleum as sole carbon source. Of 60 isolates screened for petroleum-based growth, 7 demonstrated activities by drop–collapse methodology over various types oils. From their growth profiles liquid culture during 132 h, all had first detection activity after 96 h....
The objective of this study was to standardize the high-resolution melting method for identification and discrimination Toxoplasma gondii, Sarcocystis spp., Neospora Cryptosporidium spp. by amplification 18S ribosomal DNA (rDNA) using a single primer pair. analyses were performed on individual reactions (containing from species protozoan), duplex two protozoa in each reaction), multiplex reaction four parasites reaction). proposed allowed us identify discriminate analyzing derivative,...
Strains of Toxoplasma gondii in Brazil are highly genetically diverse compared to strains from North America and Europe. Dogs epidemiologically important because they act as sentinels for T. infections humans good indicators environmental contamination. The aim this study was isolate characterize tissues naturally infected Brazilian dogs. For study, 21 blood samples were collected dogs at the Zoonosis Control Centers Ilhéus Itabuna cities, Bahia, Brazil. sera examined antibodies using...
Fecal samples from dogs residing in areas of poor living conditions Ilheus city / BA were examined for Salmonella spp June 2001 to April 2002. Eighteen (9.47%) 190 animals found be positive. 66.6% those taken one year old puppies, 56% the positive fed by table scrap, 66% did not drink treated water and 67% had a domiciliary behavior. Symptoms salmonellosis present 83% all animals. S. Gafsa represented most prevalent serovar (38.9%), followed Rubislaw (27.8%), Carrau (16.7%) enterica subsp....
Strains of Toxoplasma gondii in Brazil have high genetic diversity compared to North America and Europe. The bristle-spined porcupine, Chaetomys subspinosus, is often subject hunting for human food, but it not known whether can be a reservoir this parasite. aim study was verify the occurrence T. C. subspinosus from southern Bahia, Brazil, genetically characterize compare strains found with those isolated previous studies same region quantify their by multilocus PCR-RFLP PCR sequencing....