A5007242104- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Chronic Lymphocytic Leukemia Research
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- RNA Research and Splicing
- Chronic Myeloid Leukemia Treatments
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Virus-based gene therapy research
- Hepatitis C virus research
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Public Health and Environmental Issues
- HIV/AIDS Research and Interventions
- RNA Interference and Gene Delivery
- Viral Infections and Outbreaks Research
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Bacteriophages and microbial interactions
- Vitamin D Research Studies
Instituto de Salud Carlos III
2016-2025
Centro Nacional de Microbiologia
2008-2025
Centro de Investigación Biomédica en Red
2022-2025
Centre for Biomedical Network Research on Rare Diseases
2022-2024
Escuela Nacional de Sanidad
2010-2019
Centro de Biología Molecular Severo Ochoa
2010
Universidad Autónoma de Madrid
2010
Universidad Complutense de Madrid
1998-2002
Long-COVID is a new emerging syndrome worldwide that characterized by the persistence of unresolved signs and symptoms COVID-19 more than 4 weeks after infection even 12 weeks. The underlying mechanisms for are still undefined, but sustained inflammatory response caused SARS-CoV-2 in organ tissue sanctuaries or resemblance with an autoimmune disease within most considered hypotheses. In this study, we analyzed usefulness several demographic, clinical, immunological parameters as diagnostic...
There is a need for rapid, sensitive, and accurate diagnosis of lower respiratory tract infections in children, elderly, immunocompromised patients, who are susceptible to serious complications. The multiplex RT-nested PCR assay has been used widely simultaneous detection non-related viruses involved infectious diseases because its high specificity sensitivity. A new RT-PCR described this report. This approach includes nested primer sets targeted conserve regions human parainfluenza virus...
The clinical presentation of infections caused by the heterogeneous group respiratory viruses can be very similar. Thus, implementation virological assays that rapidly identify most important involved is great interest. A new multiplex reverse transcription nested-polymerase chain reaction (RT-PCR) assay able to detect and type different simultaneously described. Primer sets were targeted conserved regions nucleoprotein genes influenza viruses, fusion protein syncytial (RSV), hexon...
Main cause of severe illness and death in COVID-19 patients appears to be an excessive but ineffectual inflammatory immune response that may acute respiratory distress syndrome (ARDS). Vitamin D favour anti-inflammatory environment improve cytotoxic against some infectious diseases. A multicenter, single-blind, prospective, randomized clinical trial was approved with pneumonia levels 25-hydroxyvitamin (25(OH)D) 14.8 ng/ml (SD: 6.18) test antiviral efficacy, tolerance safety 10,000 IU/day...
Coumarins and structurally related compounds have been recently shown to present anti-human immunodeficiency virus, type 1 (HIV-1) activity. Among them, the dietary furanocoumarin imperatorin is in citrus fruits, culinary herbs, some medicinal plants. In this study we report that inhibits either vesicular stomatitis virus-pseudotyped or gp160-enveloped recombinant HIV-1 infection several T cell lines HeLa cells. These viruses express luciferase as a marker of viral replication. Imperatorin...
ABSTRACT HIV-1 elite controllers (EC) maintain undetectable viral loads (VL) in the absence of antiretroviral treatment. However, these subjects have heterogeneous clinical outcomes, including a proportion that loses control over time. In this work, we compared, longitudinal design, transient EC, analyzed before and after loss virological control, with persistent EC. The aim was to identify factors leading natural infection retrospective study design. Gag-specific T-cell responses were...
Abstract Background Virus neutralization by antibodies is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing serum or plasma, we developed pseudovirion particles composed the spike glycoprotein SARS-CoV-2 incorporated onto murine leukemia virus capsids a modified minimal genome encoding firefly luciferase. This assay design intended for use laboratories with biocontainment level 2 therefore circumvents need 3 that would be required...
Infection by novel coronavirus SARS-CoV-2 causes different presentations of COVID-19 and some patients may progress to a critical, fatal form the disease that requires their admission ICU invasive mechanical ventilation. In order predict in advance which could be more susceptible develop critical COVID-19, it is essential define most adequate biomarkers. this study, we analyzed several parameters related cellular immune response blood samples from 109 with who were recruited Hospitals...
There is now sufficient evidence to support that vitamin D deficiency may predispose SARS-CoV-2 infection and increase COVID-19 severity mortality. It has been suggested 3 supplementation be used prophylactically as an affordable safe strategy could added the existing standard treatment. This multicenter, single-blinded, prospective randomized pilot clinical trial aimed evaluate safety, tolerability, effectiveness of 10,000 IU/day in comparison with 2000 cholecalciferol for 14 days reduce...
Introduction The HIV regulatory protein Tat enhances viral transcription and also modifies host gene expression, affecting cell functions like cycle apoptosis. Residual expression of is detected in blood other tissues even under antiretroviral treatment. Cohort studies have indicated that, despite virologic suppression, people with (PWH) are at increased risk comorbidities linked to chronic inflammation, accelerated immune ageing, cellular senescence, sometimes associated abnormal genomic...
The human immunodeficiency virus type 1 (HIV-1) regulator Tat is essential for viral replication because it achieves complete elongation of transcripts. can be released to the extracellular space and taken up by adjacent cells, exerting profound cytoskeleton rearrangements that lead apoptosis. In contrast, intracellular has been described as protector from gene composed two coding exons yield a protein 101 amino acids (aa). First exon (1–72aa) sufficient transcript second (73–101 aa) appears...
HIV-1 replication is efficiently controlled by the regulator protein Tat (101 amino acids) and codified two exons, although first exon (1–72 sufficient for this process. can be released to extracellular medium, acting as a soluble pro-apoptotic factor in neighboring cells. However, HIV-1-infected CD4+ T lymphocytes show higher resistance apoptosis. We observed that intracellular expression of delayed FasL-mediated apoptosis both peripheral blood Jurkat cells, it an essential pathway control...
A potential strategy to cure HIV-1 infection is use latency reversing agents (LRAs) eliminate latent reservoirs established in resting CD4+ T (rCD4+) cells. As no drug has been shown be completely effective, finding new drugs and combinations are of increasing importance. We studied the effect Maraviroc (MVC), a CCR5 antagonist that activates NF-κB, on replication from latency. HIV-1-latency models based CCL19 or IL7 treatment, before were used. Latently infected primary rCD4+ central memory...
The causative mutation responsible for limb girdle muscular dystrophy 1F (LGMD1F) is one heterozygous single nucleotide deletion in the stop codon of nuclear import factor Transportin 3 gene (TNPO3). This causes a carboxy-terminal extension 15 amino acids, producing protein unknown function (TNPO3_mut) that co-expressed with wild-type TNPO3 (TNPO3_wt). has been involved transport serine/arginine-rich proteins such as splicing factors and also HIV-1 infection through interaction viral...
SARS-CoV-2 infection causes COVID-19, ranging from mild to critical disease in symptomatic subjects. It is essential better understand the immunologic responses occurring patients with most severe outcomes. In this study, parameters related humoral immune response elicited against were analysed 61 different presentations of COVID-19 who recruited Hospitals and Primary Healthcare Centres Madrid, Spain, during first pandemic peak between April June 2020. Subjects allocated as without...
The effects of the human immunodeficiency virus type 1 (HIV-1) Tat protein on cellular gene expression were analysed using a Jurkat cell line that was stably transfected with tat in doxycycline-repressible system. Expressed (aa 1-101) proved to present basically nuclear localisation, and be fully functional induce HIV LTR transactivation. also resulted protection from Tunicamycin-induced apoptosis as determined by DNA staining TUNEL assays. We applied proteomics methods investigate changes...
In HIV-1/HCV-coinfected patients, chronic HCV infection leads to an increased T-lymphocyte immune activation compared HIV-monoinfected thereby likely contributing increase HIV-1 reservoir that is the major barrier for its eradication. Our objective was evaluate influence of coinfection in viral size resting (r) CD4+ T-cells (CD25-CD69-HLADR-). Multicenter cross-sectional study 97 cART-treated including 36 patients with HIV and HCV-chronic co-infection without anti-HCV treatment, 32...
The clinical presentations of COVID-19 may range from an asymptomatic or mild infection to a critical fatal disease. Several host factors such as elderly age, male gender, and previous comorbidities seem be involved in the most severe outcomes, but also impaired immune response that causes hyperinflammatory state is unable clear infection. In order get further understanding about this response, we aimed determine association specific HLA alleles with different COVID-19. Therefore, analyzed...
HIV-1 replication results in mitochondrial damage that is enhanced during antiretroviral therapy (ART). The onset of regulated by viral protein Tat, a 101-residue codified two exons elongates transcripts. Although the first exon Tat (aa 1–72) forms itself an active protein, presence second 73–101) more competent transcriptional with additional functions. Mitochondrial overall functions were analyzed Jurkat cells stably expressing full-length (Tat101) or one-exon (Tat72). Representative...