Abstract Purpose: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for vivo quantitative imaging of receptors using near-infrared (NIR) optical imaging. The goal is provide that will minimally interfere the studied system, is, whose binding does not therapeutic agents effect on target cells minimal. Experimental Design: used three different types HER2-specific Affibody molecules [monomer...

This research describes a noninvasive, noncontact method used to quantitatively analyze the functional characteristics of tissue. Multispectral images collected at several near-infrared wavelengths are input into mathematical optical skin model that considers contributions from different analytes in epidermis and dermis layers. Through reconstruction algorithm, we can quantify percent blood given area tissue fraction is oxygenated. Imaging normal confirms previously reported values for...

In this article, a fluorescence lifetime imaging system for small animals is presented. Data were collected by scanning region of interest with measurement head, linear fiber array fixed separations between single source and several detection fibers. The goal was to localize tumors monitor their progression using specific fluorescent markers. We chose near-infrared contrast agent, Alexa Fluor 750 (Invitrogen Corp., Carlsbad, CA). Preliminary results show that the dye sensitive immediate...

Abstract Background Atrial fibrillation is a progressive arrhythmia that has become global concern affecting the quality of life millions patients. This bibliometric analysis aims to highlight top 50 most cited articles in field atrial patients, focusing on publication trends, citations, influential authors and journals, promising institutions, key contributing countries. Results The were published between 1995 2021 across 15 with an average number citations 380.5 per article. article...

Cancers overexpressing the HER2/neu gene are usually more aggressive and associated with poor prognosis. Although trastuzumab has significantly improved outcome, many tumors do not respond or acquire resistance to current therapies. To provide an alternative HER2-targeted therapy, we have developed characterized a novel recombinant protein combining HER2-specific Affibody modified Pseudomonas aeruginosa exotoxin A (PE 38), which, after binding HER2, is internalized delivered cytosol of tumor...

Noncontact optical imaging of curved objects can result in strong artifacts due to the object's shape, leading curvature biased intensity distributions. This artifact mask variations properties, and makes reconstruction optical/physiological properties difficult. In this work we demonstrate a correction method that removes recovers underlying data, without necessity measuring shape. is applicable many modalities suffer from shape-based biases. By separating spatially varying data (e.g.,...

One of the most important factors in choosing a treatment strategy for cancer is characterization biomarkers cells. Particularly, recent advances Monoclonal Antibodies (MAB) as primary-specific drugs targeting tumor receptors show that their efficacy depends strongly on biomarkers. Assessment status individual patients would facilitate selection an optimal strategy, and continuous monitoring those binding process to therapy provide means early evaluation therapeutic intervention. In this...

Anisotropy of mouse and human skin is investigated in vivo using polarized videoreflectometry. An incident beam (linearly polarized, wavelength 650 nm) focused at the sample surface. Two types tissuelike media are used as controls to verify technique: isotropic delrin highly anisotropic demineralized bone with a priori knowledge preferential orientation collagen fibers. Equi-intensity profiles light, backscattered from sample, fitted ellipses that appear follow The ratio ellipse semiaxes...

Limited data are available regarding the physicochemical dynamics of tissue hypoxia in sickle cell disease. Studies using near-infrared spectroscopy (NIRS) have reported that patients with disease (SCD) lower cerebral oxygen saturation values (rSO2) than normal individuals. The reason SCD subnormal rSO2 is not known. It may be related to degree anaemia, haemoglobin, complications and possibility different NIRS absorbance spectra normal. This study compared blood adult haemoglobin AA, SS, AS....

Human epidermal growth factor receptor 2 (HER2) overexpression in breast cancers is associated with poor prognosis and resistance to therapy. Current techniques for estimating this important characteristic use ex vivo assays that require tissue biopsies. We suggest a novel noninvasive method characterize HER2 expression vivo, using optical imaging, based on HER2-specific probes (albumin-binding domain–fused-(Z HER2:342)2 -Cys Affibody molecules [Affibody AB, Solna, Sweden], labeled Alexa...

Abstract Purpose: Advances in tumor biology created a foundation for targeted therapy aimed at inactivation of specific molecular mechanisms responsible cell malignancy. In this paper, we used vivo fluorescence lifetime imaging with HER2-targeted fluorescent probes as an alternative method to investigate the efficacy 17-DMAG (an HSP90 inhibitor) on tumors high expression HER2 receptors. Experimental Design: HER2-specific Affibody, conjugated Alexafluor 750, was injected into nude mice...

Two noninvasive methods, thermography and laser Doppler imaging (LDI), were assessed for their ability to quantitatively assess parameters of vascularity in lesions HIV-associated Kaposi's sarcoma (KS). Thermography LDI images a representative KS lesion recorded 16 patients compared normal skin either adjacent the or on contralateral side. Eleven had greater than 0.5 degrees C increased temperature 12 flux (measured by LDI) as skin. There was strong correlation between these two (R = 0.81, p...

Abstract In vivo optical imaging using fluorescently labeled self‐quenched monoclonal antibodies, activated through binding and internalization within target cells, results in excellent target‐to‐background ratios. We hypothesized that these molecular probes could be utilized to accurately report on cellular with fluorescence lifetime (FLI). Two were synthesized, consisting of the antibody trastuzumab (targeting HER2/neu) conjugated Alexa Fluor750 ratios either 1:8 or 1:1. Fluorescence...

Multispectral images of skin contain information on the spatial distribution biological chromophores, such as blood and melanin. From this, parameters volume oxygenation can be retrieved using reconstruction algorithms. Most approaches use some form pixelwise or volumetric code. We explore principal component analysis (PCA) multispectral to access in near real time. present data from healthy volunteers under arterial occlusion forearm, experiencing ischemia reactive hyperemia. Using a...

Purpose Amplification of the HER2/neu gene and/or overexpression corresponding protein have been identified in approximately 20% invasive breast carcinomas. Assessment HER2 expression vivo would advance development new HER2-targeted therapeutic agents and, potentially, facilitate choice proper treatment strategy offered to individual patient. We present novel HER2-specific probes for evaluation receptor status by near-infrared (NIR) optical imaging. Experimental Design Affibody molecules...

In this paper we examine possible data types for time resolved fluorescence enhanced diffuse optical tomography (FDOT). FDOT is a particular case of tomography, where our goal to analyze fluorophores deeply embedded in turbid medium. We focus on the relative robustness different sets noise. use an analytical model generate expected temporal point spread function (TPSF) and from this. Varying levels noise are applied TPSF before generating types. show that local more robust than global types,...

We describe a novel reconstruction algorithm based on Principal Component Analysis (PCA) applied to multi-spectral imaging data. Using numerical phantoms, two layered skin model developed previously, we found analytical expressions, which convert qualitative PCA results into quantitative blood volume and oxygenation values, assuming the epidermal thickness be known. also evaluate limits of accuracy this method when value is not show that can reliably extracted (less than 6% error) even if...

Human epidermal growth factor receptor type 2 (HER2) is a well-known biomarker that overexpressed in many breast carcinomas. HER2 expression level an important to optimize the therapeutic strategy and monitor treatment. We used albumin binding domain–fused HER2-specific Affibody molecules, labeled with Alexa Fluor750 dye, characterize vivo. Near-infrared optical imaging studies were carried out using mice subcutaneous HER2-positive tumors. Animals divided into groups of five: no treatment 12...