A5064120248- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Intensive Care Unit Cognitive Disorders
- Advanced Chemical Sensor Technologies
- Gut microbiota and health
- Microbial Metabolic Engineering and Bioproduction
- Sepsis Diagnosis and Treatment
- Cardiac, Anesthesia and Surgical Outcomes
- Diet and metabolism studies
- Chemical Reactions and Isotopes
- Anesthesia and Neurotoxicity Research
- Immune Response and Inflammation
- thermodynamics and calorimetric analyses
- Tryptophan and brain disorders
- Drug-Induced Hepatotoxicity and Protection
- Mitochondrial Function and Pathology
- Traumatic Brain Injury and Neurovascular Disturbances
- Infectious Aortic and Vascular Conditions
- Aortic aneurysm repair treatments
- Nephrotoxicity and Medicinal Plants
- Hemodynamic Monitoring and Therapy
- Alcohol Consumption and Health Effects
- Frailty in Older Adults
- Anesthesia and Sedative Agents
- Lung Cancer Research Studies
V.A. Negovsky Scientific Research Institute of General Reanimatology
2016-2025
Samara State Medical University
2023
Bashkir State Medical University
2023
Federal Research Centre of Nutrition and Biotechnology
2023
Kursk State Medical University
2023
Immanuel Kant Baltic Federal University
2023
Rostov State Medical University
2023
Sechenov University
2023
Lomonosov Moscow State University
2015-2019
Moscow State University
2014
Mechanisms of mitochondrial dysfunction in sepsis are being extensively studied recent years. During our study, concentrations microbial phenolic acids and metabolites (succinic, α-ketoglutaric, fumaric, itaconic acids) as indicators dysfunction, respectively, measured by gas chromatography–mass spectrometry (GC–MS) the blood critically ill patients at early late stages documented sepsis. The increase levels some phenylcarboxylic (phenyllactic (PhLA), p-hydroxyphenylacetic (p-HPhAA),...
Abstract Background High serum levels of certain aromatic microbial metabolites (AMM) are associated with severity and mortality in critically ill patients. Omics-based studies suggest gut dysbiosis reduced microbiome diversity critical conditions. However, the landscape is still to be outlined, not mention interplay correlation between metabolome The aim this study was analyze association fecal AMM compare them composition microbiota patients acute chronic stages. Methods In prospective...
INTRODUCTION: Microbiota metabolism disturbance, assessed by the dynamics of clinically significant aromatic metabolites in blood, plays an important role development complications after cardiac surgery, which is basis for search a new prevention strategy. OBJECTIVES: To evaluate feasibility microbial modulation using antibiotics-inhibitors protein synthesis bacterial cell infectious-inflammatory surgery. MATERIAL AND METHODS: A randomized prospective interventional study was conducted,...
Aim. To identify biomarkers for prediction and early diagnosis of infectious inflammatory complications in patients after aortic surgery. Materials methods . The study included 57 who underwent surgical procedures on the aorta its branches under cardiopulmonary bypass myocardial ischemia. cohort was divided into two groups: with an uneventful postoperative period (group 1, N=35) local surgery 2, N=22). Serum levels procalcitonin (PCT), interleukins (IL-6 IL-10), aromatic microbial...
Introduction: We hypothesized that aromatic microbial metabolites (AMM), such as phenyllactic (PhLA), p-hydroxyphenylacetic (p-HPhAA), and p-hydroxyphenyllactic (p-HPhLA) acids, contribute to the pathogenesis of septic shock. Methods: Clinical laboratory data patients with community-acquired pneumonia were obtained on intensive care unit admission next day. Patients divided into two groups based shock presence or absence. The levels AMM (PhLA, p-HPhAA, p-HPhLA, their sum, ∑3AMM),...
Indole-containing acids—tryptophan metabolites—found in serum and cerebrospinal fluid (CSF) samples of patients with diseases the central nervous system (CNS) were determined use microextraction by packed sorbent (MEPS) followed silylation gas chromatography–mass spectrometry (GC–MS) analysis. MEPS following led to reproducible formation derivatives an unsubstituted hydrogen ion indole ring, chromatographic peaks which are symmetric can be used for GC–MS analysis without additional...
Post-COVID-19 syndrome is a complex of different symptoms, which results in multisystemic impairment after the suffering from COVID-19 infection. The aim study was to reveal clinical, laboratory, and gut disorders patients with post-COVID-19 (n = 39) before taking part 14-day program rehabilitation. A complete blood count, coagulation test, chemistry, biomarkers, metabolites serum samples, dysbiosis were revealed on day admission rehabilitation, comparison variables healthy volunteers 48) or...
Postoperative complications in cardiovascular surgery remain an important unresolved problem, particular patients with aortic aneurysm. The role of the altered microbiota such is great interest. aim this pilot study was to determine whether development postoperative aneurysm related initial or acquired disorders metabolism by monitoring level some aromatic microbial metabolites (AMMs) circulating blood before and early period. comprised (n = 79), including without 36) all types 43). serum...
A review on the quantitative determination of aromatic monocarboxylic acids (AMCAs), intermediates in metabolism proteinogenic amino phenylalanine and tyrosine, body fluids is presented. The importance these compounds as potential diagnostic low-molecular-weight biomarkers some human diseases described. main methodical features current practical solutions field highly sensitive screening AMCAs using chromatography-mass spectrometry other methods actively used clinical laboratory analysis are...
The development of accurate and applicable methods to assess the severity patient condition is an urgent need in critical care medicine. goal study was determine whether quantitave evaluation phenylcarboxylic acids (PhCAs) concentrations blood might be employed treatment efficiency intensive unit. Materials methods. Clinical laboratory findings patients (n=58) with acute surgical diseases abdominal organs were registered on day admission unit during follow-up control including lactate level...
The community structure and metabolic potential of gut microbiome is not well investigated, especially in chronically critically ill patients with prolonged dependence on support systems after severe brain disorders. Microbial phenolic metabolites can target the function by direct indirect modulation inflammation. aim this study was to investigate features microbiota profile certain progression reversibility neurological disorders patients. Fecal samples were collected dynamics from such (n...
Background: Early diagnosis of postoperative complications is an urgent task, allowing timely prescribing appropriate therapy and reducing the cost patient treatment. Prognostic models based on various data could help in early after cardiac surgery. Objectives: The purpose study was to determine whether integrated approach clinical along with metabolites biomarkers had greater predictive value than built less data. Methods: included patients (n=62) admitted for planned surgery (coronary...
The search for new potential biomarkers the diagnostics of post-neurosurgical bacterial meningitis is required because difficulties in its early verification using results routine laboratory and biochemical analyses cerebrospinal fluid (CSF). goal study was to determine contents aromatic metabolites CSF samples patients (n = 82) their diagnostical significance evaluation risk meningitis. Patients with signs 30) had lower median values glucose higher cell count, neutrophils, lactate, protein,...
The profile of and dynamic concentration changes in tyrosine, phenylalanine, tryptophan metabolites blood are great interest since they could be considered potential biomarkers different disorders. Some aromatic metabolites, such as 4-hydroxyphenyllactic, 4-hydroxyphenylacetic, phenyllactic, 4-hydroxybenzoic acids have previously demonstrated their diagnostic significance critically ill patients with post-COVID-19 syndrome. In this study, a sensitive method, including serum protein...
Abstract A new approach to the quantitative analysis of aromatic metabolites in cerebrospinal fluid samples neurosurgical patients based on microextraction by packed sorbent coupled with derivatization and GC–MS was developed. Analytical characteristics such as recoveries (40–90%), limit detection (0.1–0.3 μ m ) quantitation (0.4–0.7 values, accuracy (<±20%), precision (<20%) linear correlations ( R 2 ≥ 0.99) over a 0.4–10 range concentrations demonstrated that provides results for...
A number of aromatic metabolites tyrosine and phenylalanine have been investigated as new perspective markers infectious complications in the critically ill patients intensive care units (ICUs). The goal our research was to build a multivariate model for predicting outcome regardless main pathology on day admission ICU. Eight were detected serum using gas chromatography-mass spectrometry. samples obtained from (n = 79), including survivors 44) non-survivors 35), healthy volunteers 52)....