A5048039887- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Intensive Care Unit Cognitive Disorders
- Sepsis Diagnosis and Treatment
- Diet and metabolism studies
- Tryptophan and brain disorders
- Medicinal Plants and Bioactive Compounds
- Nosocomial Infections in ICU
- Mitochondrial Function and Pathology
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolic Engineering and Bioproduction
- Cardiac, Anesthesia and Surgical Outcomes
- Antifungal resistance and susceptibility
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Metabolism and Genetic Disorders
- S100 Proteins and Annexins
- Infective Endocarditis Diagnosis and Management
- Anesthesia and Neurotoxicity Research
- thermodynamics and calorimetric analyses
- Pharmacological Effects of Natural Compounds
- Chemical Reactions and Isotopes
- Immune Response and Inflammation
V.A. Negovsky Scientific Research Institute of General Reanimatology
2016-2025
Resuscitation Council
2022
Russian Academy of Sciences
1998-2015
Academy of Medical Sciences
1998-2012
Bakulev Scientific Center for Cardiovascular Surgery Russian Academy of Medical Sciences
2003-2011
City Clinical Hospital No15 named. O. M. Filatova
1998
Abstract Background Several low-molecular-weight phenolic acids are present in the blood of septic patients at high levels. The microbial origin most human body was shown previously, but pathophysiological role is not clear. Sepsis associated with excessive production reactive oxygen species (ROS) both circulation and affected organs. In this work influence on ROS mitochondria neutrophils investigated. Methods determined by MCLA- luminol-dependent chemiluminescence. rate consumption...
BackgroundThe pathogenesis of endotoxemic tubular dysfunction with failure in urine concentration is poorly understood.Urea plays an important role the urinary concentrating mechanism and expression urea transporters UT-A1, UT-A2, UT-A3, UT-A4 UT-B essential for reabsorption.The present study attempts to assess regulation renal during severe inflammation vivo.Materials methods By agreement animal protection committee C57BL/6J, mice were injected lipopolysaccharides (LPS, 10 mg/kg) or...
A hypothesis proposed, according to which non-specific purulent-inflammatory processes are mainly caused by disturbances at low-molecular level—i.e. small molecules originating from microbes (SMOM), while SMOM homeostasis decompensation takes the leading role in complex polyfactorial pathogenesis of sepsis. The is logically justified understanding reasonableness for creation within ontogenesis system signal molecules, would serve as mediators informational exchange between microorganisms and...
The paper postulates whether the phenomenon of integration metabolism in man and his microbiome exists. author's previously hypothesized fact that small molecules from microbes are involved human homeostasis [1, 2] has found confirmation subsequent experimental clinical studies. interactions bacterial metabolites with body, which natural harmonious health, shown to become distorted different diseases, may be present critical conditions at most worsening disintegration results multiple organ...
Mechanisms of mitochondrial dysfunction in sepsis are being extensively studied recent years. During our study, concentrations microbial phenolic acids and metabolites (succinic, α-ketoglutaric, fumaric, itaconic acids) as indicators dysfunction, respectively, measured by gas chromatography–mass spectrometry (GC–MS) the blood critically ill patients at early late stages documented sepsis. The increase levels some phenylcarboxylic (phenyllactic (PhLA), p-hydroxyphenylacetic (p-HPhAA),...
Abstract Background High serum levels of certain aromatic microbial metabolites (AMM) are associated with severity and mortality in critically ill patients. Omics-based studies suggest gut dysbiosis reduced microbiome diversity critical conditions. However, the landscape is still to be outlined, not mention interplay correlation between metabolome The aim this study was analyze association fecal AMM compare them composition microbiota patients acute chronic stages. Methods In prospective...
Gut microbiome in critically ill patients shows profound dysbiosis. The most vulnerable is the subgroup of chronically (CCI) - those suffering from long-term dependence on support systems intensive care units. It important to investigate their as a potential reservoir opportunistic taxa causing co-infections and morbidity factor. We explored dynamics composition CCI by combining "shotgun" metagenomics with chromosome conformation capture (Hi-C). Stool samples were collected at 2 time points...
INTRODUCTION: Microbiota metabolism disturbance, assessed by the dynamics of clinically significant aromatic metabolites in blood, plays an important role development complications after cardiac surgery, which is basis for search a new prevention strategy. OBJECTIVES: To evaluate feasibility microbial modulation using antibiotics-inhibitors protein synthesis bacterial cell infectious-inflammatory surgery. MATERIAL AND METHODS: A randomized prospective interventional study was conducted,...
Aim. To identify biomarkers for prediction and early diagnosis of infectious inflammatory complications in patients after aortic surgery. Materials methods . The study included 57 who underwent surgical procedures on the aorta its branches under cardiopulmonary bypass myocardial ischemia. cohort was divided into two groups: with an uneventful postoperative period (group 1, N=35) local surgery 2, N=22). Serum levels procalcitonin (PCT), interleukins (IL-6 IL-10), aromatic microbial...
Introduction: We hypothesized that aromatic microbial metabolites (AMM), such as phenyllactic (PhLA), p-hydroxyphenylacetic (p-HPhAA), and p-hydroxyphenyllactic (p-HPhLA) acids, contribute to the pathogenesis of septic shock. Methods: Clinical laboratory data patients with community-acquired pneumonia were obtained on intensive care unit admission next day. Patients divided into two groups based shock presence or absence. The levels AMM (PhLA, p-HPhAA, p-HPhLA, their sum, ∑3AMM),...
High concentration of sepsis-associated aromatic microbial metabolites (AMM) stands as a prognostically unfavorable factor, indicating the progression multiple organ dysfunction and an increased risk death in patients with sepsis septic shock. This study is based on hypothesis that excess AMM caused by metabolic alterations (dysfunction) intestinal microbiota. The aim this was to compare potential normobiota pathobiota bio-transform amino acids tyrosine phenylalanine, such phenyllactic...
Radical changes in the composition, diversity and metabolic activity of gut microbiome critically ill patients most probably affect adversely outcome treatment. Microbiota dysfunction may be a predictor presumably main cause infectious complications sepsis. Clinicists use objective scales for evaluation patient condition severity including specific parameters disorders organs systems; however, microbiota function is not considered and, hence, evaluated. Technical capabilities recent decade...
Indole-containing acids—tryptophan metabolites—found in serum and cerebrospinal fluid (CSF) samples of patients with diseases the central nervous system (CNS) were determined use microextraction by packed sorbent (MEPS) followed silylation gas chromatography–mass spectrometry (GC–MS) analysis. MEPS following led to reproducible formation derivatives an unsubstituted hydrogen ion indole ring, chromatographic peaks which are symmetric can be used for GC–MS analysis without additional...
Post-COVID-19 syndrome is a complex of different symptoms, which results in multisystemic impairment after the suffering from COVID-19 infection. The aim study was to reveal clinical, laboratory, and gut disorders patients with post-COVID-19 (n = 39) before taking part 14-day program rehabilitation. A complete blood count, coagulation test, chemistry, biomarkers, metabolites serum samples, dysbiosis were revealed on day admission rehabilitation, comparison variables healthy volunteers 48) or...
The human gastrointestinal tract is inhabited by a diverse and dense symbiotic microbiota, the composition of which result host-microbe co-evolution co-adaptation. This tight integration creates intense cross-talk signaling between host microbiota at cellular metabolic levels. In many genetic or infectious diseases balance may be compromised resulting in erroneous communication. Consequently, metabolome, includes gut different health disease states terms microbial products metabolites...
Postoperative complications in cardiovascular surgery remain an important unresolved problem, particular patients with aortic aneurysm. The role of the altered microbiota such is great interest. aim this pilot study was to determine whether development postoperative aneurysm related initial or acquired disorders metabolism by monitoring level some aromatic microbial metabolites (AMMs) circulating blood before and early period. comprised (n = 79), including without 36) all types 43). serum...
Introduction Urokinase receptor (uPAR, CD87), a glycosylphosphatidylinositol-anchored protein, is considered to play an important role in inflammation and fibrinolysis.The Gram-negative bacterium Burkholderia pseudomallei able survive replicate within leukocytes causes melioidosis, cause of pneumonia-derived community-acquired sepsis Southeast Asia.We here investigated the expression function uPAR both patients with septic melioidosis murine model experimental melioidosis.Methods Using...
The development of accurate and applicable methods to assess the severity patient condition is an urgent need in critical care medicine. goal study was determine whether quantitave evaluation phenylcarboxylic acids (PhCAs) concentrations blood might be employed treatment efficiency intensive unit. Materials methods. Clinical laboratory findings patients (n=58) with acute surgical diseases abdominal organs were registered on day admission unit during follow-up control including lactate level...
The community structure and metabolic potential of gut microbiome is not well investigated, especially in chronically critically ill patients with prolonged dependence on support systems after severe brain disorders. Microbial phenolic metabolites can target the function by direct indirect modulation inflammation. aim this study was to investigate features microbiota profile certain progression reversibility neurological disorders patients. Fecal samples were collected dynamics from such (n...