A5003529989- Antibiotic Resistance in Bacteria
- Lichen and fungal ecology
- Microbial Natural Products and Biosynthesis
- Pneumocystis jirovecii pneumonia detection and treatment
- Pharmaceutical and Antibiotic Environmental Impacts
- Tuberculosis Research and Epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Computational Drug Discovery Methods
- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Antimicrobial Peptides and Activities
- Drug Transport and Resistance Mechanisms
- Essential Oils and Antimicrobial Activity
- Probiotics and Fermented Foods
- Cancer therapeutics and mechanisms
- Phenothiazines and Benzothiazines Synthesis and Activities
- Chromium effects and bioremediation
- Antifungal resistance and susceptibility
- Curcumin's Biomedical Applications
- Cell Adhesion Molecules Research
- Bryophyte Studies and Records
- Enzyme function and inhibition
University of L'Aquila
2014-2024
San Salvatore Hospital
2020
To evaluate the effect of two combined antimicrobial drugs, one method currently in use is checkerboard assay a 96-well microplate, which gives good vivo estimation drug-drug combination effect. Appropriate and consolidated methods are described numerous scientific publications are, however, turn, laborious time-spending, specifically for setting microplate preparation. Each drug every must be prepared dispensed individually several steps, often limiting its terms consumed materials working...
Bacterial resistance has become a worldwide concern after the emergence of metallo-β-lactamases (MBLs). They represent one major mechanisms bacterial against beta-lactam antibiotics. Among MBLs, New Delhi metallo-β-lactamase-1 NDM-1, most prevalent type, is extremely efficient in inactivating nearly all-available antibiotics including last resort carbapenems. No inhibitors for NDM-1 are currently available therapy, making spread producing strains serious menace. With this perspective, we...
Recent decades have witnessed a dramatic increase of multidrug resistant (MDR) bacteria, compromising the efficacy available antibiotics, and continual decline in discovery novel antibacterials. We recently reported first library benzo[b]thiophen-2-ylboronic acid inhibitors sharing broad spectrum activity against β-lactamases (BLs). The ability these compounds to inhibit structurally mechanistically different types has been here investigated. An extensive X-ray crystallographic analysis...
Purpose: This study aims to investigate the antifungal activity and mechanism of action ozonized oil eye drops in liposomes (Ozodrop), commercialized as lubricant for treatment dry syndrome inflammation. The was tested against four clinical Candida species: C albicans, glabrata, krusei, orthopsilosis. Methods: drop solution ascertained by microdilution method accordance with EUCAST obtaining minimum inhibitory concentration Ozodrop. further investigated albicans measuring cell vitality,...
Abstract The emergence and dissemination of multidrug resistant (MDR) pathogens to nearly all available antibiotics poses a significant threat in clinical therapy. Among them, Klebsiella pneumoniae isolates overexpressing KPC‐2 carbapenemase are the most worrisome, extending bacterial resistance last‐resort carbapenems. In this study, we investigate molecular recognition requirements active site by small phenylboronic acid derivatives. Four new derivatives were designed tested against KPC‐2....
The purpose of this study was to investigate the presence and distribution integron-carrying isolates among Enterobacteriaceae resistant β-lactam antibiotics collected from a wastewater effluent city L'Aquila (Italy). A total 471 were during period 2 years (2005-2006). class 1 integrons investigated by colony blot hybridization using specific probes labelled with dUTP-fluoresceine kit. variable region analyzed polymerase chain reaction DNA sequencing in 24 different random amplified...
The in vitro antibacterial activities of eight compounds isolated from lichens, collected several Southern regions Chile (including Antarctica), were evaluated against methicillin-resistant clinical isolates strains Staphylococcus aureus, haemolyticus and warneri. minimum inhibitory concentrations, calculated microdilution, ranging 8 µg mL−1 for sphaerophorin to 1024 fumarprotocetraric acid. These findings suggest, however, that the natural lichens are good candidates individuation novel...
The emergence of bacteria that co-express serine- and metallo- carbapenemases is a threat to the efficacy available β-lactam antibiotic armamentarium. 4-amino-1,2,4-triazole-3-thione scaffold has been selected as starting chemical moiety in design small library β-Lactamase inhibitors (BLIs) with extended activity profiles. synthesised compounds have validated vitro against class A serine β−Lactamase (SBLs) KPC-2 B1 metallo β−Lactamases (MBLs) VIM-1 IMP-1. Of derivatives, four showed...
Abstract (+)‐Usnic acid (UA) is a natural substance that displays pharmacological activity, but it barely soluble in water, so was included liposomes order to study its properties. First, the effects of phospholipid structure and loading methodology on UA entrapment efficacy were evaluated. Then, physicochemical biological properties (UA delivery Staphylococcus aureus bacterial cells) different liposome formulations containing structurally related amphiphiles derived from L‐ prolinol fully...
The study investigates the regulatory effects exhibited by lysate of Lactobacillus sakei pro-Bio65 (4%; L.SK) on human conjunctival epithelial (HCE) cell line.Trypan blue and methylthiazol tetrazolium (MTT) methods were used to assess growth viability. Mitochondrial membrane potential was assessed JC-1 staining cytofluorimetric detection methods. antioxidant pattern intracellular reactive oxygen species (ROS) levels analyzed spectrophotometric spectrofluorimetric NF-κB luciferase activity...