A5080146887- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Crustacean biology and ecology
- Invertebrate Immune Response Mechanisms
- Reproductive biology and impacts on aquatic species
- Neurobiology and Insect Physiology Research
- Animal Genetics and Reproduction
- Physiological and biochemical adaptations
- Aquaculture disease management and microbiota
- Genetic diversity and population structure
- Fish Biology and Ecology Studies
- Metabolomics and Mass Spectrometry Studies
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Protease and Inhibitor Mechanisms
- Autophagy in Disease and Therapy
- Effects of Environmental Stressors on Livestock
- MicroRNA in disease regulation
- Aquatic life and conservation
- Marine Biology and Environmental Chemistry
- RNA modifications and cancer
- Insect Resistance and Genetics
- Peptidase Inhibition and Analysis
- Sperm and Testicular Function
- Cell Adhesion Molecules Research
Chinese Academy of Fishery Sciences
2015-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Suzhou Municipal Hospital
2017-2024
Nanjing Medical University
2010-2024
Chongqing Medical University
2024
Affiliated Hospital of Liaoning University of Traditional Chinese Medicine
2024
Jiangxi Science and Technology Normal University
2024
Jiangsu Province Hospital
2023-2024
Fudan University
2023
Wenzhou Medical University
2007-2022
Background The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China, even whole of Asia. androgenic gland produces hormones that play crucial roles sexual differentiation to maleness. This study the first de novo M. nipponense transcriptome analysis using cDNA prepared from mRNA isolated gland. Illumina/Solexa was used for sequencing. Methodology and Principal Finding total volume RNA sample more than 5 ug. We generated 70,853,361 high quality reads...
Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of quantitative results determined by stability reference genes used. Recent studies have investigated suitable for some crustaceans under various conditions, but Macrobrachium nipponense are currently lacking. In this study, we selected following seven from among 35 commonly housekeeping as candidate qPCR temporal and spatial expression: EIF (eukaryotic translation initiation factor 5A), 18S (18S...
Objective To assess the global changes in and characteristics of transcriptome long noncoding RNAs (LncRNAs) heart tissue, whole blood plasma during failure (HF) association with expression paired coding genes. Methods Here we used microarray assay to examine LncRNAs deregulated heart, blood, HF mice. We confirmed by quantitative PCR. Results revealed a number that were HF, which suggests potential role HF. Strikingly, patterns LncRNA differed between other tissue was associated length all...
The oriental river prawn, Macrobrachium nipponense, is an economically important shrimp in China. Male prawns have higher commercial value than females because the former grow faster and reach larger sizes. It therefore to reveal sex-differentiation development mechanisms of prawn enable genetic improvement.We sequenced 293.3 Gb raw Illumina short reads 405.7 Pacific Biosciences long reads. final whole-genome assembly Oriental was ∼4.5 size, with predictions 44,086 protein-coding genes. A...
The doublesex and mab-3 related transcription factor (DMRT) gene family involvement in sex development is widely conserved from invertebrates to humans. In this study, we identified a DM (Doublesex/Mab-3)-domain Macrobrachium nipponense, which named MniDMRT11E because it has many similarities phylogenetically close relationships with the arthropod DMRT11E. Amino acid alignments structural prediction uncovered conservation putative active sites of domain. Real-time PCR analysis showed that...
Abstract Background Macrobrachium nipponense is an economically important species of freshwater shrimp in China. Unlike other marine shrimps, the ovaries adult female M. can mature rapidly and periodically during reproductive period, but resulting high stocking densities environmental deterioration negatively impact harvest yield economic benefits. To better understand ovary development , we performed systematic transcriptome sequencing five different stages ovarian maturation. Results We...
Abstract Background Macrobrachium nipponense is a freshwater prawn of economic importance in China. Its reproductive molt crucial for seedling rearing and directly impacts the industry’s efficiency. 20-hydroxyecdysone (20E) controls various physiological behaviors crustaceans, among which initiation molt. Previous studies have shown that 20E plays vital role regulating oviposition M. . However, research on molecular mechanisms underlying still limited. Results A total 240.24 Gb data was...
Abstract The oriental river prawn, Macrobrachium nipponense , is an important commercial aquaculture resource in China. During breeding season, short ovary maturation cycles of female prawns cause multi-generation reunions ponds and affect the growth females representing individual miniaturization (known as autumn -propagation). These reproductive characteristics pose problems for large - scale farming. To date, molecular mechanisms reproduction regulation M . remain unclear. address this...
White spot syndrome virus (WSSV) is one of the most devastating pathogens cultured shrimp, responsible for massive loss its commercial products worldwide. The oriental river prawn Macrobrachium nipponense an economically important species that widely farmed in China and adult prawns can be infected by WSSV. However, molecular mechanisms host pathogen interaction remain unknown. There urgent need to learn between M. WSSV which will able offer a solution controlling spread Next Generation...
Photoperiod is an important factor in stimulating broiler performance commercial poultry practice. However, the mechanism by which photoperiod affects of chickens has not been adequately explored. The current study evaluated effects three different regimes (short day (LD) = 8 h light, control (CTR) 12.5 and long (SD) 16 light) on cecal microbiota roosters sequencing bacterial 16S rRNA genes. At phylum level, dominant bacteria were Firmicutes (CTR: 68%, SD: 69%, LD: 67%) Bacteroidetes 25%,...
Abstract The transformer‐2 gene ( tra‐2 ) plays a key role in the sexual differentiation regulatory hierarchy. In this study, homologs designated as Mntra‐2 was cloned and characterized from Macrobrachium nipponense . full‐length cDNA of consists 1724 bp with an open reading frame ORF encoding 192 amino acids, 827 5′‐untranslated region UTR 318 3′‐ predicated molecular mass 20.805 kDa estimated theoretical isoelectric point 10.36. deduced acid sequence shares high homology Penaeus monodon...
Abstract Forkhead box protein L2 ( Foxl2 ) has dramatic effects on early differentiation and development of the female gonad in mammals fish through repression SOX9 expression. In this study, we aimed to isolate a full‐length cDNA sequence encoding from Macrobrachium nipponense Mn‐Foxl2 investigated its gene function. The was 2097 bp with an open reading frame 1740 bp, 579 amino acids. Phylogenetic analysis revealed considerable evolutionary divergence between fish. may be involved gonadal...
The oriental river prawn, Macrobrachium nipponense, is an important commercial aquaculture resource in China. In order to overwinter, M. nipponense displays decreased physiological activity and less consumption of energy. Sudden warming would trigger molting cause extensive death, resulting huge economic losses. Therefore, it great practical significance study the mechanism prawns. Molt-inhibiting hormone gene (MIH) plays a major role regulating crustaceans. this study, full length MIH cDNA...
Background: Fibrosis results in excessive buildup of extracellular matrix proteins along with abnormalities structure and is partly derived by a process involving transforming growth factor β (TGF-β) called endothelial-to-mesenchymal transition (EndMT). We investigated whether the aldosterone receptor-blocker spironolactone could abrogate TGF-β-induced fibrosis EndMT underlying mechanism. Methods: Human umbilical vein endothelial cells (HUVECs) were divided into 5 groups for treatment:...