A5101954925- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Genetic diversity and population structure
- Aquaculture Nutrition and Growth
- Molecular Biology Techniques and Applications
- Crustacean biology and ecology
- Forensic and Genetic Research
- Neurobiology and Insect Physiology Research
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Invertebrate Immune Response Mechanisms
- Identification and Quantification in Food
- Fish Biology and Ecology Studies
- Epigenetics and DNA Methylation
- Medicinal Plant Pharmacodynamics Research
- Poxvirus research and outbreaks
- Insect Resistance and Genetics
- Animal Behavior and Reproduction
- Collagen: Extraction and Characterization
- Estrogen and related hormone effects
- interferon and immune responses
- Advanced Computational Techniques and Applications
- Rangeland Management and Livestock Ecology
- Plant and animal studies
- Virus-based gene therapy research
Chinese Academy of Fishery Sciences
2012-2023
Ministry of Agriculture and Rural Affairs
2022-2023
Guiyang Medical University
2021
Inner Mongolia Medical University
2014
Hubei Academy of Agricultural Sciences
2014
Animal Science Research Institute
2014
Jiangsu Academy of Agricultural Sciences
2012
Fujian Medical University
2005
The doublesex and mab-3 related transcription factor (DMRT) gene family involvement in sex development is widely conserved from invertebrates to humans. In this study, we identified a DM (Doublesex/Mab-3)-domain Macrobrachium nipponense, which named MniDMRT11E because it has many similarities phylogenetically close relationships with the arthropod DMRT11E. Amino acid alignments structural prediction uncovered conservation putative active sites of domain. Real-time PCR analysis showed that...
Abstract Forkhead box protein L2 ( Foxl2 ) has dramatic effects on early differentiation and development of the female gonad in mammals fish through repression SOX9 expression. In this study, we aimed to isolate a full‐length cDNA sequence encoding from Macrobrachium nipponense Mn‐Foxl2 investigated its gene function. The was 2097 bp with an open reading frame 1740 bp, 579 amino acids. Phylogenetic analysis revealed considerable evolutionary divergence between fish. may be involved gonadal...
The eyestalk of crustacean species secretes many hormones, affecting the process reproduction, molting, metabolism glucose, and other functions in crustaceans. In this study, important metabolic pathways candidate genes involved male sexual development were identified through performing transcriptome profiling analysis testis after ablation from Macrobrachium nipponense . histological observations revealed that became vigorous ablation, indicating hormones secreted by have negative effects...
The steroid 17α-methyltestosterone (MT) inhibits ovarian function and is often used to induce sex reversal artificially in vertebrates. In the present study, different concentrations of MT were added as dietary supplementation, effects on ratio, growth, gonadal development examined. After 40 days, ratio (male:female) each group increased at degrees with 50 (1.36:1), 100 (1.57:1), 200 (2.61:1) mg/kg MT, neo-males testis–ovary coexistence observed group. Furthermore, could female reversion...
Gem-associated protein 2-like isoform X1 (GEM) was previously predicted to be involved in the sexual development of male Macrobrachium nipponense. In this study, we analyze GEM functions depth using quantitative polymerase chain reaction (qPCR), situ hybridization, and RNA interference (RNAi). The full-length Mn-GEM cDNA sequence 1018 base pairs (bp) long with an open reading frame 777 bp encoding 258 amino acids. qPCR analysis different tissues developmental stages showed that highly...
Sex reversal induced by 17β-estradiol (E2) has shown the potential possibility for monoculture technology development. The present study aimed to determine whether dietary supplementation with different concentrations of E2 could induce sex in M. nipponense, and select sex-related genes performing gonadal transcriptome analysis normal male (M), female (FM), sex-reversed prawns (RM), unreversed (NRM). Histology, analysis, qPCR were performed compare differences gonad development, key...
Male oriental river prawns (Macrobrachium nipponense) grow faster than females, and therefore, reach larger sizes by harvest time. Histological observations have indicated that the sex-differentiation sensitive period (which includes formation of androgenic gland, testis, ovary) is from post-larvae (PL) developmental stage for M. nipponense. In this study, we prepared four microRNA (miRNA) mRNA libraries using samples collected (PL7 to PL16) perform RNA-sequencing identifying sex-related...
Male-specifically inherited Y-STRs, harboring the features of haploidy and lack crossing over, have gained considerable attention in population genetics forensic investigations. Goldeneye® Y-PLUS kit was a recently developed amplification system focused on genetic diversity 36 Y-chromosomal short tandem repeats (Y-STRs) East Asians. However, no data corresponding were reported China. Here, Y-STRs first genotyped 400 unrelated healthy Tai-Kadai-speaking Bouyei male individuals. A total 371...
The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China.The androgenic gland produces hormones that play crucial roles the differentiation of crustaceans to male sex.MicroRNA (miRNA) post-transcriptionally regulates many protein-coding genes, influencing biological and metabolic processes.However, currently, there no published data identifying miRNA M. nipponense.In this study, we identified novel nipponense.Using high-throughput Illumina Solexa...
This study was performed by using D-loop region sequences to investigate genetic diversity and population structure across eight representative groups of M. nipponense from south north China. Eight populations comprising a total 144 individuals were Songhua River (SHJ), Yellow (HH), Yangtze (YZ), Pearl (ZJ), Weishan Lake (WSH), Taihu (TH), Bosten (BH) Wanquan (WQH). The 1130-bp exhibited higher content A + T (79.04%) than C G (20.95%). In addition, 239 variable sites 73 haplotypes detected....
Background Y-chromosomal short tandem repeats (Y-STRs) are widely used in paternity identification, pedigree investigation, and human population genetic history.Aim To investigate the Y-STR polymorphisms a typical Miao population, explore differentiation between reference groups.Subjects methods We detected 36 Y-STRs genotyping 455 unrelated individuals from Guizhou province, analysed 76 groups.Results A total of 369 alleles were obtained, allele frequencies ranged 0.0022 to 0.9802. In...
Cyclin A (CycA) plays essential roles in regulating multiple steps of the cell cycle, and it affects gonad development mammals invertebrates. Previous RNA interference (RNAi) analysis revealed that knocking-down expression CycA female oriental river prawns (Macrobrachium nipponense) inhibited ovarian development. was also predicted to have regulatory reproductive male M. nipponense based on significant changes Mn-CycA after eyestalk ablation. The goal this study investigate potential...
In the present study, quantitative real-time PCR was employed to investigate expression profiling and difference of ESR1 ESR2 in ovaries Shaoxing duck Jingjiang during three laying stages. Results showed levels were increased from age first egg 500 days both breeds. The lower than that for 180 days, it higher duck. converse two results suggest mediate process ducks, may play a more important role ovary egg-laying stages be closely related performance ducks.
This article presents a study of D-loop sequences to characterize the genetic diversity wild Macrobrachium nipponense populations in Yixing natural waters including two reservoirs (Hengshan reservoir, HS; Youche YC), 3 brooks (Linjin Dang, LJD; Magong Dushan MDD; Yangshan YSD) and shallow lakes (Dongjiu lake, DJ; Xijiu XJ; Tuanjiu XJ), compared differentiation population structure with Taihu Lake (TH), Yangtze River (YZ), main local artificially bred varieties “Taihu No. 2” (TH-2). A 747 bp...
Pyruvate dehydrogenase E1 ( PDHE1 ) is thought to play essential roles in energy metabolism, and a previous study suggested that it also has potential regulatory male sexual development the oriental river prawn, Macrobrachium nipponense . In this study, we used rapid amplification of cDNA ends, quantitative polymerase chain reaction (qPCR), situ hybridization, western blotting, RNA interference (RNAi), histological analyses assess functions Mn-PDHE1 M. The full sequence was 1,614 base pairs...
Abstract Background Male‐specifically inherited Y‐STRs have been widely used in population genetics and forensic investigations. Methods We genotyped analyzed Y chromosome haplotypes of 408 unrelated Tibeto‐Burman‐speaking Yi male individuals from Guizhou using Goldeneye ® Y‐PLUS kit. Population comparisons between the 67 reference groups were performed via AMOVA, MDS, phylogenetic relationship reconstruction. Results A total 389 alleles 396 could be detected, allelic frequencies ranged...
Objective To investigate the influence of tumor metastasis suppressor gene [homo sapiens longevity assurance homologue 2/tumor 1 (LASS2/TMSG-1)] on invisive and metastatic potentiality prostate cancer cell lines.Methods First,small RNA interference technology was used to silence expression LASS2/TMSG-1 in human line PC-3M-2B4 (low potentiality),and then real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) technique immunocytochemistry were screen most efficient small...
Abstract Three genes were predicted to be potentially involved in the male sexual development M . nipponense , including Gem-associated protein 2-like isoform X1 (GEM), Ferritin peptide, and DNA polymerase zeta catalytic subunit (Rev3). In this study, we aimed investigate their novel functions depth. The full-length cDNA sequence of Mn-GEM was 1,018 bp, encoding 258 amino acids. partial Mn-Rev3 6,832 1,203 Tissue distribution indicated that all these three have higher expression level testis...