A5066888860- Parasitic Infections and Diagnostics
- Crustacean biology and ecology
- Neurobiology and Insect Physiology Research
- Amoebic Infections and Treatments
- Aquaculture Nutrition and Growth
- Invertebrate Immune Response Mechanisms
- Physiological and biochemical adaptations
- Helminth infection and control
- Coccidia and coccidiosis research
- Aquaculture disease management and microbiota
- Clostridium difficile and Clostridium perfringens research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Metabolomics and Mass Spectrometry Studies
- Reproductive biology and impacts on aquatic species
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Identification and Quantification in Food
- Microbial infections and disease research
- Toxoplasma gondii Research Studies
- Insect Resistance and Genetics
- Ferroptosis and cancer prognosis
- Cancer-related gene regulation
- Congenital Anomalies and Fetal Surgery
- Research on scale insects
- Reproductive Biology and Fertility
Henan Provincial Center for Disease Control and Prevention
2022-2025
Henan Agricultural University
2020-2025
Ministry of Agriculture and Rural Affairs
2022-2025
Chinese Academy of Fishery Sciences
2021-2024
Shanghai Ocean University
2022-2024
Shanghai Institute of Nutrition and Health
2020
Chinese Academy of Tropical Agricultural Sciences
2011
Ministry of Agriculture and Agro Based Industry
2011
Ministère de l'Agriculture et de la Souveraineté alimentaire
2011
Environment and Plant Protection Research Institute
2011
Eimeria are obligate protozoan parasites, and more than 1,500 species have been reported. However, genomes lag behind many other eukaryotes since obtaining oocysts is difficult due to a lack of sustainable in vitro culture, highly repetitive sequences, mixed infections. To address this challenge, we used whole-genome amplification single oocyst followed by long-read sequencing obtained chromosome-level genome tenella. The assembled was 52.13 Mb long, encompassing 15 chromosomes 46.94% repeat...
Abstract Background A close connection between a protozoan parasite and the balance of other gut microbes host has been demonstrated. The calves may be naturally co-infected with many parasites, co-effects parasites on intestinal remain unclear. This study aims to preliminarily reveal relationship in calves. Methods Fecal samples were collected from four bloody diarrhea, watery seven normal calves, microbial flora analyzed by whole-genome sequencing. Protozoal detected metagenome sequences...
Giardia duodenalis is one of the major causes diarrhea among humans, especially in young children. Statistical analysis revealed that pooled prevalence G. dogs, and cats was 9.72% (10,921/112383), 15.60% (7510/48140), 14.53% (1125/7740), respectively. Unquestionably, canine-specific assemblages C D feline-specific assemblage F were dominant genotypes dogs cats, Additionally, zoonotic (A B) 23.07% (875/3792) 41.42% (169/408), respectively, implying potential transmission from to human...
The eyestalk of crustacean species secretes many hormones, affecting the process reproduction, molting, metabolism glucose, and other functions in crustaceans. In this study, important metabolic pathways candidate genes involved male sexual development were identified through performing transcriptome profiling analysis testis after ablation from Macrobrachium nipponense . histological observations revealed that became vigorous ablation, indicating hormones secreted by have negative effects...
Abstract The eyestalk of crustaceans, such as Macrobrachium nipponense , contains many neurosecretory hormones affecting the process reproduction, molting, metabolism glucose, and other functions. In this study, important metabolic pathways candidate genes involved in male sexual development were selected from M. . methodology performing long-read next generation transcriptome sequencing androgenic gland after ablation. qPCR analysis revealed that mRNA expression Mn-IAG was significantly...
Blastocystis is a usual intestinal protist that always found in humans and various animals. Currently, the prevalence of migratory whooper swan (Cygnus cygnus) unknown. In our research, we aimed to determine occurrence, prevalence, subtype distribution dynamic transmission mechanisms China. We also assessed zoonotic potential isolates, as well possible routes impact this organism on One Health perspective. Fecal samples (n = 770) were collected from swans inhabiting Sanmenxia Swan Lake...
Variability in food availability leads to condition-dependent investments reproduction. This study is aimed at understanding the metabolic response and regulatory mechanism of female
Blastocystis sp., Enterocytozoon bieneusi, and Giardia duodenalis are three common zoonotic intestinal parasites, cattle important hosts of these protozoa. In this study, 1632 fecal samples were collected from dairy farms in Heilongjiang Province, China, screened for E. G. using polymerase chain reaction. Of these, 149 (9.13%) positive pathogens, including 104 (6.40%), 22 (1.35%), 23 (1.41%) duodenalis, respectively. Based on partial SSU rRNA gene sequencing analysis, sp. found, a total nine...
Giardia duodenalis is an important zoonotic parasite that causes economic losses to animal husbandry and threatens public health. In the present study, a total of 1466 fresh fecal samples were collected from sheep ( n = 797), goats 561) beef cattle 108) in Southwest Inner Mongolia, China. was initially screened via nested polymerase chain reaction (PCR) targeting β-giardin bg ) gene, -positive subjected PCR amplification glutamate dehydrogenase gdh triose phosphate isomerase tpi genes. A...
The mud crab, Scylla paramamosain, has abundant nutrients in the ovary, where numerous lipids accumulate during ovarian maturation. However, mechanism behind accumulation of ovary crab maturation is largely unknown. This study conducted a comparative transcriptome analysis ovaries crabs at various stages A total 63.69 Gb clean data was obtained, with Q30 93.34%, and 81,893 unigenes were identified, including 10,996 differentially expressed genes (DEGs). After KEGG enrichment these DEGs, MAPK...
Molting and ovulation are the basic processes responsible for growth reproduction of Macrobrachium nipponense ; however, molecular mechanisms molting in M. poorly understood. The present study aimed to use MnFtz-f1 as starting point phenomena at level. full-length cDNA sequence was 2,198 base pairs (bp) length with an open reading frame 1,899 bp encoding 632 amino acids. Quantitative real-time PCR analysis showed that highly expressed ovary cleavage stage on fifth day after hatching. In vivo...
Cryptosporidium is a leading cause of diarrheal disease and mortality in young children worldwide. invades small intestinal epithelial cells forms unique intracellular niche, process that may alter gut microbes their production metabolites. However, the mechanism interactions between microbes, metabolites, parasites poorly understood. Here, we first characterized impacts infection on microbiota using microbiome-to-metabolome association study. BALB/c mice were gavaged with muris, fecal...
Abstract In order to provide valuable guidelines for the conservation of germplasm Lateolabrax maculatus , genetic diversity and population structure analysis were evaluated eight geographic populations along coastal regions China, using 11 microsatellite DNA markers. The parameters obtained showed that, can be clustered into two groups, Northern group Southern group, concordant with their geographical positions. UPGMA tree constructed according Nei’s distance discriminant principal...
To investigate the embryonic development of mud crab Scylla paramamosain , we analyzed three critical parameters: egg color of, embryo morphology (through conventional and laser scanning confocal microscopy), distribution cell divisions. During development, exhibited a progressive transition, shifting from orange to reddish-orange, then brown, before ultimately darkening black. Each displayed spherical shape, measuring approximately 280 μm in diameter, characterized by smooth surface devoid...
The CYP306a1 gene is a member of the Halloween genes in cytochrome P450 supergene family, which play an important regulatory role synthesis 20-hydroxyecdysone (20E) arthropods. In this study, we cloned full-length DNA sequence (Mn-CYP306a1) oriental river prawn Macrobrachium nipponense. encoded 600 amino acids, including typical CYP450 conserved domains. We detected expression Mn-CYP306a1 different tissues male and female M. nipponense by qRT-PCR. results showed that was highly expressed its...
The sesquiterpenoid hormone methyl farnesoate (MF) plays a vital role during crustacean development, which is mainly evidenced by its varied titers different developmental stages. However, the biosynthesis pathways of MF remain obscure to some extent. In this study, we identified complete and related pathway genes in Scylla paramamosain, including three involved acetyl-CoA metabolism, eight mevalonate pathway, five sesquiterpenoids synthesis methionine cycle pathway. Bioinformatics, genomic...
Cryptosporidium parvum is a zoonotic parasite of the intestine and poses threat to human animal health. However, it difficult obtain large number oocysts for genome sequencing using in vitro culture. To address this challenge, we employed strategy whole-genome amplification 10 followed by long-read obtained high-quality assembly C. IIdA19G1 subtype isolated from pre-weaning calf with diarrhea. The assembled was 9.13 Mb long encompassed eight chromosomes six capped telomeric sequences at one...