A5050693504- Neuroblastoma Research and Treatments
- Sarcoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Renal Diseases and Glomerulopathies
- Cell Adhesion Molecules Research
- Cancer therapeutics and mechanisms
- Tumors and Oncological Cases
- Congenital Diaphragmatic Hernia Studies
- Genomic variations and chromosomal abnormalities
- Glioma Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Platelet Disorders and Treatments
- Vasculitis and related conditions
- Bone Tumor Diagnosis and Treatments
- Parvovirus B19 Infection Studies
- Soft tissue tumor case studies
- Lung Cancer Research Studies
- Prenatal Screening and Diagnostics
- Renal cell carcinoma treatment
- Pregnancy and preeclampsia studies
- Salivary Gland Tumors Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
University of Toronto
2015-2024
Chulalongkorn University
2015-2024
King Chulalongkorn Memorial Hospital
2016-2024
Hospital for Sick Children
2011-2020
SickKids Foundation
2010-2020
Faculty (United Kingdom)
2019
Canada Research Chairs
2015
University of Chicago
2012
University of Illinois Chicago
2012
University of Calgary
1993-2012
In the Children's Oncology Group, risk group assignment for neuroblastoma is critical therapeutic decisions, and patients are stratified by International Neuroblastoma Staging System stage, MYCN status, ploidy, Shimada histopathology, diagnosis age. Age less than 365 days has been associated with favorable outcome, but recent studies suggest that older age cutoff may improve prognostic precision.To identify optimal cutoff, we retrospectively analyzed data from Pediatric Group biology study...
Increased expression of P-glycoprotein is associated with multidrug resistance (MDR) in many cell lines. Significant levels have been detected a number human tumors. The purpose this study was to determine whether correlates both response chemotherapy and prognosis soft tissue sarcoma childhood. In retrospective study, biopsy samples from 30 cases rhabdomyosarcoma (RMS) undifferentiated (US) treated at Hospital for Sick Children Toronto were analyzed using semiquantitative...
Multidrug resistance in chemotherapy for cancer is characterized by increased genetic expression of P-glycoprotein, which acts as an ATP-dependent drug-efflux pump. To determine whether P-glycoprotein levels are prognostic value such cases, we measured these immunohistochemically a retrospective study sequential tumor samples from 67 children with neuroblastoma.
The primary objective of Children's Oncology Group study P9641 was to demonstrate that surgery alone would achieve 3-year overall survival (OS) ≥ 95% for patients with asymptomatic International Neuroblastoma Staging System stages 2a and 2b neuroblastoma (NBL). Secondary objectives focused on other low-risk NBL those who required chemotherapy according protocol-defined criteria.Patients underwent maximally safe resection tumor. Chemotherapy reserved with, or at risk for, symptomatic disease,...
Some reports suggest that autologous hematopoietic stem cell transplantation holds potential for treatment of renal diseases such as lupus nephritis, but the safety delivering various types (hematopoietic, mesenchymal, and endothelial precursors) is not well established. Here, we report a case nephritis treated by direct injection cells recovered from peripheral blood. The patient developed masses at sites hematuria. We suspected transitional carcinoma nephrectomy revealed were...
PURPOSE Although a high rate of spontaneous regression is observed in infants with stage D(S) neuroblastoma (NB), survival not uniform. To determine the prognostic relevance age at diagnosis, therapy, and tumor biology NB, we reviewed Pediatric Oncology Group (POG) experience. PATIENTS AND METHODS A review patients diagnosed NB registered on POG protocols was performed. Survival according to treatment, determined. RESULTS Between 1987 1996, 110 had an estimated 3-year 85% +/- 4%; 71% 8% for...
Abstract Objective Wegener's granulomatosis (WG) is a predominantly small‐vessel vasculitis associated with antineutrophil cytoplasmic antibodies (ANCAs). There are few reports describing its clinical features and outcome in children. We report on the experience at single tertiary referral center over 21 years. Methods conducted retrospective chart review of all patients diagnosed WG The Hospital for Sick Children between 1984 2005. Results Twenty‐five were identified. Median age diagnosis...
Abstract Neuroblastoma is a heterogeneous pediatric tumor thought to arise from the embryonic neural crest. Identification of cell responsible for propagating neuroblastomas essential understanding this often recurrent, rapidly progressing disease. We have isolated and characterized putative tumor-initiating cells 16 tumors bone marrow metastases patients in all neuroblastoma risk groups. Dissociated or grew as spheres conditions used culture crest stem cells, were capable self-renewal,...
Abstract Background Human osteosarcoma is the most common pediatric bone tumor. There limited understanding of molecular mechanisms underlying oncogenesis, and a lack good diagnostic as well prognostic clinical markers for this disease. Recent discoveries have highlighted potential role number genes including: RECQL4 , DOCK5 SPP1 RUNX2 RB1 CDKN1A P53 IBSP LSAMP MYC TNFRSF1B BMP2 HISTH2BE FOS CCNB1 CDC5L . Methods Our objective was to assess relative expression levels these 16 biomarkers...
Altered gene expression in tumors can be caused by copy number alterations to DNA or mutation affecting coding regulatory regions of genes. However, epigenetic events may also influence expression. Malignant cells show major disruptions methylation profiles, which are manifested as aberrant hypermethylation hypomethylation promoters, well global genomic hypomethylation. In this study we performed integrative whole-genome analysis number, promoter and using 10 osteosarcomas. We identified...
Abstract Flt1, an “fms-like tyrosine kinase” receptor, has been suggested to play active role in vascular endothelial growth factor (VEGF)–mediated autocrine signaling of tumor and angiogenesis. Here, we used a neuroblastoma model investigate the VEGF/Flt1 hypoxia-mediated cell survival, drug resistance, vivo SK-N-BE(2), highly malignant line resistant hypoxia-induced apoptosis expresses Flt1 but lacks VEGFR2 expression. We found that 24-hour hypoxia (<0.1% O2) alone (no serum...
The cellular composition of crescents in glomerular disease is controversial. role podocytes crescent formation has been especially difficult to study because typically lose their characteristic terminally differentiated phenotype under conditions, making them identify. We reasoned that the intermediate filament protein nestin, a marker progenitor cells recently identified podocytes, may allow investigation podocyte involvement crescents. In series 35 biopsies with crescentic disease, all...
Persistent proteinuria, chronic renal failure, and focal segmental glomerulosclerosis developed in three children with solitary kidneys. Two of these were born unilateral The third had bilateral reflux underwent a nephrectomy reimplantation the remaining ureter; persistent proteinuria 7 years later. It is postulated that hyperperfusion critical number glomeruli during childhood may be mechanism responsible for production patients.
Abstract Conventional cytogenetic studies have shown that osteosarcomas (OSs) are often highly aneuploid, with a large number of both structural and numerical chromosomal alterations. To investigate the complexity OS karyotypes in detail, we applied spectral karyotyping (SKY) to series 14 primary tumors four established cell lines. A total 531 rearrangements were identified by SKY, which 300 breakpoints could be assigned specific chromosome band. There was an average 38.5 SKY per tumor....
Many families with X-chromosome linked hereditary nephritis (HN) have mutations in the gene on X chromosome that codes for alpha 5 chain of collagen type IV. Canine X-linked HN is an animal model human HN. To study 5(IV) this model, we used nucleotide sequence published cDNA to construct sets primers covering approximately 95% complete cDNA. from both affected and normal dog kidneys was amplified by PCR nine overlapping regions. The encoding noncollagenous domain NC1 hybridized 93% identical...