A5091634778- Rabies epidemiology and control
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Viral Infections and Vectors
- Cellular transport and secretion
- Venomous Animal Envenomation and Studies
- Neuroscience of respiration and sleep
- Insect symbiosis and bacterial influences
- Parasitic Infections and Diagnostics
- COVID-19 Clinical Research Studies
- Neuroscience, Education and Cognitive Function
- Dermatological and COVID-19 studies
- Bacillus and Francisella bacterial research
- Yersinia bacterium, plague, ectoparasites research
- Yeasts and Rust Fungi Studies
- Medical and Biological Sciences
- Insect-Plant Interactions and Control
- Infectious Encephalopathies and Encephalitis
- AI in Service Interactions
- Plant Virus Research Studies
- Biotin and Related Studies
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Social Robot Interaction and HRI
- Poxvirus research and outbreaks
Instituto Nacional de Salud
2015-2025
University of Illinois Urbana-Champaign
2024
Instituto Nacional de Salud
2001-2003
Universidad Nacional de Colombia
1989-1991
Background: The Zika virus (ZIKV) is an arbovirus linked to “Congenital Syndrome” and a range of neurodevelopmental disorders (NDDs), with microcephaly as the most severe manifestation. Milder NDDs, such autism spectrum delays in neuropsychomotor language development, often go unnoticed neonates, resulting long-term social academic difficulties. Murine models ZIKV infection can be used mimic part motor cognitive deficits observed humans. These evaluated through behavioral tests, enabling...
Rabies has been an enigmatic disease of the nervous system because microscopic findings in brain tissue are not paralleled by severity clinical illness. The calcium binding protein calbindin (CB) is a neuronal marker great interest neuroanatomy and neuropathology. CB-ir neurons striatum cerebral cortex gabaergic cells. In present work CB-immunoreactivity was evaluated brains normal rabies-infected mice. infection caused loss CB-immunostaining cortical supragranular layers as well striatum....
The microtubule-associated protein MAP-2 is an integral part of the cytoskeleton and plays important role in neural morphogenesis.This essential component dendritic cytoskeleton, especially adult brain, its expression can be altered under experimental or pathological conditions.The purpose this study was to evaluate effect infection with rabies virus on immunoreactivity cerebral cortex mice.The mice were inoculated animals sacrificed when disease reached advanced stage, together uninfected...
Rabies is a viral infection that targets the nervous system, specifically neurons. The clinical manifestations of disease are dramatic and their outcome fatal; paradoxically, conventional histopathological descriptions reveal only subtle changes in affected tissue. Some researchers have considered pathophysiology rabies based more on biochemical than structural alterations, as case with some psychiatric diseases. However, we believe it has been necessary to resort other methods allow us...
Introduction: Rabies is a fatal infectious disease of the nervous system; however, knowledge about pathogenic neural mechanisms in rabies scarce. In addition, there are few studies pathology spinal cord.Objective: To study distribution calcium binding proteins calbindin and parvalbumin assessing effect virus infection on their expression cord mice.Materiales y methods: Mice were inoculated with virus, by intracerebral or intramuscular route. The was extracted to perform some crosscuts which...
A Zika virus (ZIKV) strain was isolated from an acute febrile patient during the epidemics in Colombia. The intraperitoneally inoculated into BALB/c mice, and 7 days postinoculation, neurological manifestations ZIKV infection brain were demonstrated. reported genome sequence is highly related to strains circulating Americas.
The family Tymoviridae comprises positive-sense RNA viruses, which mainly infect plants. Recently, a few Tymoviridae-like viruses have been found in mosquitoes, feed on vertebrate sources. We describe novel virus, putatively named, Guachaca virus (GUAV), isolated from Culex pipiens and quinquefasciatus species of mosquitoes collected the rural area Santa Marta, Colombia. After cytopathic effect was observed C6/36 cells, extracted processed through NetoVIR next-generation sequencing protocol,...
Zika virus (ZIKV) disease continues to be a threat public health, and it is estimated that millions of people have been infected there more cases serious complications than those already reported. Despite many studies on the pathogenesis ZIKV, several genes involved in malformations associated with viral infection are still unknown. In this work, morphological molecular changes cortex cerebellum mice ZIKV were evaluated. Neonatal BALB/c inoculated intraperitoneally, respective controls...
Los coronavirus son una familia de virus que se caracterizan por producir afectaciones respiratorias y gastrointestinales en animales seres humanos. El actual SARS-CoV-2, agente infeccioso la COVID-19, pertenece a un subgrupo denominado betacoronavirus del hacen parte el SARS-CoV MERS-CoV, responsables epidemias 2002 2012, respectivamente.Estos también pueden infectar sistema nervioso debido su afinidad con enzima convertidora angiotensina humana 2 (ACE2), cual expresa neuronas células...
Some clinical features of rabies and experimental evidence from cell culture laboratory animals suggest impairment gabaergic neurotransmission. Several types neurons occur in the cerebral cortex. They can be identified by three neuronal markers: calcium binding proteins (CaBPs) parvalbumin (PV), calbindin (CB) calretinin (CR). Rabies virus spreads throughout cortex; however, cytopathic effects on are unknown. The expression calcium-binding (CR) was studied frontal cortex mice. effect...
The Golgi silver impregnation technique is a simple histological procedure that reveals complete three-dimensional neuron morphology. This method based in the formation of opaque intracellular deposits chromate obtained by reaction between potassium dichromate and nitrate (black reaction). Camillo Golgi, its discoverer, Santiago Ramón y Cajal main exponent, shared Nobel Prize Medicine Physiology 1906 for their contribution to knowledge nervous system structure, Their successes were largely...
The neurological signs of rabies are very dramatic. Nevertheless, the infected brain manifests only subtle histological changes.The neuronal morphology in cerebral cortex rabies-infected mice was examined by means Golgi technique for detection neuropathy.Two groups were inoculated with rabies-one street virus isolated from an dog and second fixed CVS (challenge standard) virus. At terminal phase illness, animals sacrificed staining perfusion paraformaldehyde. Next, brains treated coronal...
Rabies is a lethal disease caused by neurotropic virus that produces inconspicuous morphological changes hardly observable with conventional histopathology.The fatal outcome rabies could be attributed to specific biochemical severely impact neuronal function.The nuclear protein (NeuN) has become widely used marker for the research and histopathological diagnosis of nervous system diseases.To evaluate distribution NeuN in motor cortex normal rabies-infected mice adult ICR were inoculated...
Introduction. The standard procedure for rabies diagnosis requires fresh samples of infected brain to be analyzed by two techniques, direct immunofluorescence and inoculation in mice. Rabies-infected, aldehyde-fixed tissues can examined immunohistochemistry, but the required commercial antibodies are scarce expensive.Objectives. An anti-rabies antiserum was produced tested evaluate effectiveness antigen detection aldehyde preserved tissue.Materials methods. Rabbits were inoculated with a...
There are few studies of infection by rabies virus in the olfactory bulb (OB). This work was carried out with purpose establishing time required to detect antigens OB mouse, after intramuscular inoculation and evaluate effect on expression three proteins: calbindin (CB), parvalbumin (PV) glial fibrillary acidic protein (GFAP). Mice were inoculated intramuscularly hind limbs. Every 8 hours, 72 hours postinoculation (p.i.), animals sacrificed perfusion paraformaldehyde coronal sections...
Objetivos: Estudiar el efecto de la infección con rabia sobre ultraestructura dendrítica las neuronas piramidales corteza cerebral en ratones inoculados virus por vía intramuscular. Métodos: Ratones adultos y solución vehículo sin (controles) fueron fijados perfusión intracardiaca, una que contenía paraformaldehído al 4% glutaraldehído 2%, cuando los animales infectados manifestaron signos avanzados enfermedad. Los encéfalos extraídos cortados plano coronal un vibrátomo. Fragmentos pequeños...
Introducción: La calbindina (CB) es una proteína reguladora del calcio intracelular y la célula de Purkinje cerebelo neurona con más alta concentración CB. Se ha demostrado pérdida inmunorreactividad a CB en diferentes áreas sistema nervioso ratones inoculados virus rabia, pero faltaba estudiar este fenómeno el cerebelo. Objetivo: Determinar efecto inoculación rabia sobre expresión células ratones. Metodología: inocularon por vía intramuscular. sacrificaron los animales cuando alcanzaron...
GABAergic neurons synthesize and release gamma-aminobutyric acid, the predominant inhibitory neurotransmitter in brain. Certain clinical signs of rabies previous experimental studies have suggested that viral infections affect host system.The effect virus infection on expression GABA was evaluated mouse cerebral cortex.Adult mice were inoculated by intramuscular injection with standard strain (CVS virus). The animals sacrificed terminal stage illness perfused 4% paraformaldehyde 1%...
Rabies diagnosis is mainly made on fresh brain tissue postmortem by means of the direct immunofluorescence test. However, in some cases, it not possible to use this technique, given that affected nervous goes through a degradation process, due problems handling and transport samples. For reason, preservation time rabies virus inclusions was assessed, as well immunoreactivity ultrastructure viral particles with degradation. Brains mice inoculated control were processed for conventional...
Abstract Smart-environments deploy an ecology of smart devices such as voice-assistants (VA), telehealth portals, and smart-fireplaces to support aging in place by providing assistance, remote-assessment, restoration. Although asking for is a foundational task within environment, the socioemotional implications assistance largely undefined. This study (N=51, observations=204, age:50-80, 54%female), conducted simulated “home office,” employed 2x2 (fireplace vs. not × neurologist...
Introduction: The calcium-binding protein calbindin (CB) plays a critical role in intracellular calcium metabolism. In previous study we demonstrated that the infection with fixed rabies virus caused loss of CB expression mouse brain during terminal state disease, 7-8 days after intramuscular inoculation. Objectives: To determine also appears early stages and if it is consequence immediate to appearance viral antigens brain. Materials methods: Mice were inoculated virus, by route, left hind...