A5064277459- Gut microbiota and health
- Zebrafish Biomedical Research Applications
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal motility and disorders
- Cardiomyopathy and Myosin Studies
- Hereditary Neurological Disorders
- Diet and metabolism studies
- Dermatology and Skin Diseases
- Cytomegalovirus and herpesvirus research
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Bone Tumor Diagnosis and Treatments
- Birth, Development, and Health
- Cardiac electrophysiology and arrhythmias
- Dietary Effects on Health
- Herpesvirus Infections and Treatments
- Health, psychology, and well-being
- Sarcoma Diagnosis and Treatment
- Pregnancy and preeclampsia studies
- Infant Nutrition and Health
- Genetic diversity and population structure
- Chromosomal and Genetic Variations
- Cutaneous lymphoproliferative disorders research
- Cancer Genomics and Diagnostics
Medical University of Graz
2016-2025
BioTechMed-Graz
2021
University of Graz
2011-2012
Graz University of Technology
2002-2008
In patients with ulcerative colitis (UC), alterations of the intestinal microbiota, termed dysbiosis, have been postulated to contribute inflammation. Fecal microbiota transplantation (FMT) has used as effective therapy for recurrent Clostridium difficile also caused by dysbiosis. The aims present study were investigate if UC benefit from FMT and dysbiosis can be reversed.Six chronic active nonresponsive standard medical treated colonoscopic administration. Changes in colonic assessed 16S...
Increasing evidence indicates the involvement of inflammation and coagulation in cancer progression metastases. Inflammatory biomarkers hold great promise for improving predictive ability existing prognostic tools patients. In present study, we investigated several inflammatory indices with regard to their relevance predicting clinical outcome soft tissue sarcoma (STS) Three hundred forty STS patients were divided into a training set (n = 170) validation 170). Besides well-established...
Hereditary sensory neuropathy type I (HSN I) is an axonal form of autosomal-dominant hereditary motor and distinguished by prominent loss that leads to painless injuries. Unrecognized, these can result in delayed wound healing osteomyelitis, necessitating distal amputations. To elucidate the genetic basis HSN subtype a family which mutations few known genes had been excluded, we employed massive parallel exon sequencing 14.3 Mb disease interval on chromosome 14q. We detected missense...
Background & Aims Diseases of the human gastrointestinal (GI) tract are often accompanied by diarrhea with profound alterations in GI microbiota termed dysbiosis. Whether dysbiosis is due to disease itself or accompanying remains elusive. With this study we characterized net effects osmotic on composition absence disease. Methods We induced four healthy adults oral administration polyethylene glycol 4000 (PEG). Stool as well mucosa specimens were collected before, during and after 16S...
In lung transplant patients undergoing immunosuppression, more than one human cytomegalovirus (HCMV) genotype may emerge during follow-up, and this could be critical for the outcome of HCMV infection. Up to now, many cases infection with multiple genotypes were probably overlooked due limitations current genotyping approaches. We have now analyzed mixed-genotype infections in 17 clinical samples from 9 using highly sensitive ultradeep-pyrosequencing (UDPS) technology. UDPS was performed at...
The distal hereditary motor neuropathies (dHMNs) are a heterogeneous group of neurodegenerative disorders affecting the lower motoneuron. In family with both autosomal-dominant dHMN and type V (dHMN/dHMN-V) present in three generations, we excluded mutations all genes known to be associated phenotype through Sanger sequencing defined potential loci linkage analysis. Whole-exome two affected individuals revealed single candidate variant within linking regions, i.e., splice-site alteration...
To identify the disease-causing gene responsible for an autosomal dominantly inherited Charcot–Marie–Tooth neuropathy subtype in a family excluded mutations common genes, we used array-based sequence capture to simultaneously analyse disease-linked protein coding exome at chromosome 14q32. A missense mutation fibulin-5, encoding widely expressed constituent of extracellular matrix that has essential role elastic fibre assembly and been shown cause cutis laxa, was detected as only novel...
Microbiome research and improvements in high throughput sequencing technologies revolutionize our current scientific viewpoint. The human associated microbiome is a prominent focus of clinical research. Large cohort studies are often required to investigate the composition its changes multitude diseases. Reproducible analyses large samples require standardized protocols study design, sampling, storage, processing data analysis. In particular, effect sample storage on actual results critical...
Metabolic syndrome is associated with disturbances in gut microbiota composition. We aimed to investigate the effect of Lactobacillus casei Shirota (LcS) on composition, barrier integrity, intestinal inflammation and serum bile acid profile metabolic syndrome. In a single-centre, prospective, randomised controlled pilot study, 28 subjects received either LcS for 12 weeks (n = 13) or no 15). Data were compared healthy controls 16). Gut composition was characterised from stool using 454...
Chordomas are rare, low to intermediate grade malignant bone tumors of the axial skeleton. Current treatment options limited surgical procedures, as chordomas largely resistant conventional radiation and chemotherapy. Cell lines valuable tools for exploring molecular mechanisms involved in tumorigenesis they have a fundamental impact on development new anticancer agents. To date, only two chordoma cell exist world-wide. In present study we report third line, MUG-Chor1, well corresponding...
Corpus-dominant lymphocytic gastritis (LyG) is characterized by CD8+ T-cell infiltration of the stomach epithelium a so far uncharacterized mechanism. Although Helicobacter pylori typically undetectable in LyG, patients respond to H. antibiotic eradication therapy, suggesting non-H. microbial trigger for disease. Comparative microbiota analysis specimens from and healthy controls precluded involvement LyG but identified Propionibacterium acnes as possible disease trigger. In addition,...
An adverse intrauterine environment can result in permanent changes the physiology of offspring and predispose to diseases adulthood. One such exposure, gestational diabetes mellitus (GDM), has been linked development metabolic disorders cardiovascular disease offspring. Epigenetic variation, including DNA methylation, is recognised as a leading mechanism underpinning fetal programming we hypothesised that this plays key role fetoplacental endothelial dysfunction following exposure GDM....
Ex vivo generation of red blood cells (cRBCs) is an attractive tool in basic research and for replacing components donated by volunteers. As a prerequisite the survival cRBCs during storage as well circulation, quality membrane utmost importance. Besides cytoskeleton embedded proteins, lipid bilayer critical integrity. Although suffer from increased fragility, studies investigating content their are still lacking. We investigated profile CD34+ human stem compared to native (nRBCs)...
Abstract Calreticulin ( CALR ) mutations present the main oncogenic drivers in JAK2 wildtype (WT) myeloproliferative neoplasms (MPN), including essential thrombocythemia and myelofibrosis, where mutant (MUT) is increasingly recognized as a suitable mutation-specific drug target. However, our current understanding of its mechanism-of-action derived from mouse models or immortalized cell lines, cross-species differences, ectopic over-expression lack disease penetrance are hampering...
Chordomas are rare mesenchymal tumors occurring exclusively in the midline from clivus to sacrum. Early tumor detection is extremely important as these resistant chemotherapy and irradiation. Despite continuous research efforts surgical excision remains main treatment option. Because of often challenging anatomic location early enable complete resection reduce high incidence local recurrences. The aim this study was explore whether DNA methylation, a well known epigenetic marker, may play...
Abstract Background Aggression is an adaptive behaviour that animals use to protect offspring, defend themselves and obtain resources. Zebrafish, like many other animals, are not able recognize in the mirror typically respond their own reflection with aggression. However, aggression all-or-nothing phenomenon, some individuals displaying high levels of against image, while others show none at all. In current work, we have investigated genetic basis by using a classic forward genetics approach...
Background and Purpose The use of analgosedatives in critically ill patients carries the risk impairing gastrointestinal (GI) propulsion could thereby lead to sepsis. gut microbiota can influence GI motility, but whether microbial dysbiosis modifies peristalsis impairment by analgosedative drugs has not yet been analysed. This question was addressed guinea pig small intestine following a decrease bacterial load antibiotic pretreatment. Experimental Approach Guinea pigs were enorally (within...
Maternal metabolic factors are increasingly recognized as critical pre-conceptional determinants of fertility outcomes. To investigate how health influences female fertility, we investigated the molecular composition follicular fluid (FF), with a focus on microRNA (miRNA) expression. Blood and FF samples from 15 women undergoing controlled ovarian stimulation were examined in pilot study. Clinical traits (glucometabolic markers, lipid profiles, liver function inflammatory cytokines, hormonal...