Lateral and out-of-plane organization of cholesterol its effect on regulating the physicochemical properties zwitterionic phospholipid model membranes have been investigated by a pressure–area isotherm study from Langmuir monolayer, atomic force microscopy (AFM), X-ray reflectivity (XRR) measurements supported binary monolayer films. The systematic studies phospholipids subsequent extraction excess Gibbs free energy (ΔGexc) revealed mechanism interaction molecular cooperativeness for...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-association of class I major histocompatibility complex molecules in liposome and cell surface membranesAbhijit Chakrabarti, Janos Matko, Noorul A. Rahman, B. George Barisas, Michael EdidinCite this: Biochemistry 1992, 31, 7182–7189Publication Date (Print):August 11, 1992Publication History Published online1 May 2002Published inissue 11 August...

Proteomics studies to identify proteins in the erythrocyte cytosol have been largely affected by huge abundance of hemoglobin (Hb), which masks detection other 2-D gel-based separation. We depleted Hb effectively from using cation exchange chromatography and detected more than 600 protein spots hemolysate 2-DE. so far identified 59 Hb-depleted normal erythrocytes, including 10 not before.

We have studied the conformational stability of two homologous membrane skeletal proteins, erythroid and non-erythroid spectrins, in their dimeric tetrameric forms respectively during unfolding presence urea guanidine hydrochloride (GuHCl). Fluorescence circular dichroism (CD) spectroscopy been used to study changes intrinsic tryptophan fluorescence, anisotropy, far UV-CD extrinsic fluorescence bound 1-anilinonapthalene-8-sulfonic acid (ANS). Chemical both proteins were reversible could be...

We have investigated the organization and dynamics of functionally important tryptophan residues erythroid spectrin in native denatured conditions utilizing wavelength-selective fluorescence approach. observed a red edge excitation shift (REES) 4 nm for tryptophans case its state. This indicates that are localized microenvironment restricted mobility, regions surrounding offer considerable restriction to reorientational motion water dipoles around excited state tryptophans. Interestingly,...

In (hemoglobin, Hb) HbEβ-thalassemia, HbE (β-26 Glu→Lys) interacts with β-thalassemia to produce clinical manifestation of varying severity. This is the first proteomic effort study changes in protein levels erythrocytes isolated from HbEβ-thalassemic patients compared normal.We have used 2-DE and MALDI-MS/MS-based techniques investigate differential proteome profiling membrane Hb-depleted fraction cytosolic proteins peripheral blood samples HbEβ-thalassemia normal volunteers.Our showed that...

Visceral leishmaniasis (VL) is a deadly parasitic diseases caused by Leishmania donovani; it major health problem in many countries. A lack of proper understanding the disease biology, poor diagnostic methods and increasing drug resistance are main reasons for growing burden VL infection. Comparative plasma proteomics relatively useful technique that can be used to investigate disease-associated alterations help host responses against pathogens, might management diagnosis.In this study,...

A new application of second harmonic light scattering to probe protein physisorption on a gold nanoparticle surface in aqueous buffer is reported. The free energies adsorption, the number molecules adsorbed and binding affinity moderate size protein, alcohol dehydrogenase (ADH), small insulin, have been determined using change scattered signal as function binding. Four different nanoparticles from 15 60 nm were used determine effect energy change, constant surface. All shown increase with an...

Hemoglobin A(2) (alpha(2)delta(2)) is an important hemoglobin variant which a minor component (2-3%) in the circulating red blood cells, and its elevated concentration beta-thalassemia useful clinical diagnostic. In major, where there beta-chain production failure, HbA(2) acts as predominant oxygen deliverer. has two more features. (1) It resistant to thermal denaturation than HbA, (2) it inhibits polymerization of deoxy sickle (HbS). E (E26K(beta)), formed result splice site mutation on...

AbstractThere is a compelling need to image pancreas cancer at an early stage. Human cells display elevated levels of KRAS protein due high copy numbers mRNA, and insulin-like growth factor 1 receptor (IGF1R) overexpression IGF1R mRNA. Therefore we hypothesized that could be detected in vivo with single probe targets both mRNA IGF1R. Because positron emission tomography (PET) sensitive imaging technique, designed incorporating the positron-emitting nuclide 64Cu. The KRAS-specific...

Sickle cell disease (SCD) is a hemolytic disorder caused by mutation in beta-globin gene and affects millions of people worldwide. Though clinical manifestations the are quite heterogeneous, many them occur due to erythrocyte sickling at reduced oxygen concentration vascular occlusion mediated via blood adhesion vessel wall. We have followed proteomic approach resolve differentially regulated proteins cytosol. The deregulated mainly fall group chaperone such as heat shock protein 70, alpha...

Insulin receptor (IR) and class I major histocompatibility complex molecules associate with one another in cell membranes, but the functional consequences of this association are not defined. We found that IR human (HLA-I) liposome membranes affinity for insulin its tyrosine kinase activity increase as HLA:IR ratio increases over range 1:1 to 20:1. The same relationship between function was a series B-LCL lines. HLA-I depends upon presence free HLA heavy chains. All effects noted were...

AbstractDensity-dependent spectrin binding to dimyristoylphosphatidylcholine/dimyristoylphosphatidylethanolamine (DMPC/DMPE) small uni-lamellar vesicles (SUVs) has been directly evaluated in this work from the increase extent of quenching tryptophan fluorescence at two different temperatures, above and below main phase transition temperatures (Tm). Results studies phospholipid SUVs indicated that dissociation constant Kd, increased 45±7 nM pure DMPC 219±20 DMPC/DMPE (50:50) SUVs, both gel...

Spectrin, the major constituent protein of erythrocyte membrane skeleton, exhibits chaperone activity by preventing irreversible aggregation insulin at 25 °C and that alcohol dehydrogenase 50 °C. The dimeric spectrin two subunits, α-spectrin β-spectrin prevent such appreciably better, 70% in presence an insulin:spectrin ratio 1:1, than tetramer 25%. Our results also show binds to denatured enzymes α-glucosidase alkaline phosphatase during refolding reactivation yields are increased...