Abstract A sensitive and specific liquid chromatography‐atmospheric pressure chemical ionization‐mass spectrometry (LC‐APCI‐MS) method for the simultaneous determination of spironolactone its active metabolite canrenone in human plasma has been developed validated. After addition estazolam as internal standard (IS), samples were extracted with methylene chloride : ethyl acetate mixture (20 80, v/v) separated by high‐performance chromatography (HPLC) on a reversed‐phase C 18 column mobile...

Asbestos has been confirmed as a major pollutant in asbestos-mining areas that are located western China. In general, asbestos-fibre dust will is released into the environment due to effect of intensive industrial activities and improper environmental management, such health residents around mining jeopardised. A typical asbestos area served an example this study analyse content fibre morphology soil air samples area. The effects pollution on human were also assessed based U.S. Superfund...

A rapid, simple and sensitive high performance liquid chromatography with positive ion electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) was first developed validated to simultaneously determine paracetamol (PAR, CAS 103-90-2), amantadine hydrochloride (ATH, 665-66-7), caffeine (CAF, 58-08-2) chlorpheniramine maleate (CPM, 113-92-8) in human plasma using tramadol (TMH, 22204-88-2) as internal standard (IS). Following methanolinduced protein precipitation, the analytes were...

The aim of this study was to characterize and compare the percutaneous penetration kinetics lidocaine (L) prilocaine (P) in two local anesthetic formulations by vivo microdialysis coupled with HPLC. system for studying calibrated a no-net-flux method vitro retrodialysis vivo, respectively. A dosage 0.2 g/cm2 an in-house P–L formulation (2.5% 2.5% prilocaine, methylcellulose-based) commercially available Eutectic Mixture Local Anesthesia (EMLA, carbopol-based) separately but symmetrically...

Abstract A rapid, sensitive and specific high‐performance liquid chromatography‐electrospray ionization mass spectrometry (LC/ESI‐MS) method was developed validated for the first time to determine concentration of lafutidine in human plasma. After addition diazepam (the internal standard, IS) 1 M sodium hydroxide solution 0.5‐ml plasma sample, extracted from with n ‐hexane : isopropanol (95 5, v/v). The organic layer evaporated residue redissolved 200‐µl mobile phase. analyte...

Abstract An assay based on protein precipitation and liquid chromatography/tandem mass spectrometry (LC‐MS/MS) has been developed validated for the quantitative analysis of lisinopril in human plasma. After addition enalaprilat as internal standard (IS), plasma samples were prepared by one‐step using perchloric acid followed an isocratic elution with 10 m ammonium acetate buffer (pH adjusted to 5.0 acetic acid)–methanol (70:30, v/v) a Phenomenex Luna 5µC 18 (2) column. Detection was...

The aim was to evaluate the bioequivalence of topically applied Acyclovir (ACV) creams using dermal microdialysis (DMD) in a pig model. Three ACV (3%), ACV1, ACV2 and ACV3, were administrated on dorsum pigs, DMD sampling technique used continuously collect microdialysate. concentration microdialysate measured by HPLC concentration-time profiles calculate pharmacokinetic parameters. results showed that 90% confidence interval (CI) ratio AUC0–4 h ACV3 between 88.2 105.7%, which within...

Abstract This paper describes a novel method for the sensitive and selective determination of fudosteine in human plasma. The involves derivatization step with 9‐fluorenylmethyl chloroformate (FMOC‐Cl) borate buffer detection based on high‐performance liquid chromatography‐electrospray ionization mass spectrometry (LC/ESI/MS). After acetonitrile‐induced protein precipitation plasma samples, was derivatized FMOC‐Cl, then extracted by ethyl acetate, evaporated, reconstituted injected using an...

The aim of the present study was to compare bioavailability clindamycin (CAS 18323-44-9) from three hydrochloride 21 462-39-5) capsules (clindamycin 75 mg capsule as test 1 preparation, 150 2 preparation and a commercially available original drug reference preparation) in 24 Chinese healthy male volunteers, aged between 22 28. conducted according randomized, double-blind, 3-period, 3-treatment, 3-sequence, single-dose, crossover design with wash-out phase 7 days. Blood samples for...

A rapid, sensitive and selective HPLC–MS/MS method was developed validated for the quantification of cetirizine dihy-drochloride (CAS 83881-51-0) in human plasma using mosapride citrate as internal standard (IS, CAS 112885-42-4). Following liquid–liquid extraction, ana-lytes were separated a mobile phase consisting methanol aqueous ammonium acetate solution (10 mM) (60 : 40, v/v) on reverse C18 column analyzed by triple-quadrupole mass spectrometer selected reaction monitoring (SRM) mode...

The bioavailability of a new terbinafine (CAS 91161-71-6) preparation was compared with commercially available original (reference) the drug in 19 Chinese healthy male volunteers. study performed an open, randomized, single blind two-sequence, two-period crossover design. Under fasting conditions, each subject received oral dose 250 mg as test or reference formulation 7-day washout period between two preparations. plasma concentrations were analyzed by sensitive liquid...

The aim of the present study was to compare bioavailability escitalopram (CAS 128196-01-0) from two oxalate 219861-08-2) tablets (escitalopram 10 mg tablet as test preparation and commercially available original drug reference preparation) in 20 Chinese healthy male volunteers, aged between 19 27. conducted according an open, randomized, single blind, 2-way crossover design with a wash-out phase 14 d. Blood samples for pharmacokinetic profiling were taken up 156 h post-dose, plasma...

A sensitive and highly selective liquid chromatography-mass spectrometry (LC-MS) method was developed to determine nitrendipine (4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3, 5-pyridinedicarboxylic acid ethyl methyl ester, CAS 39562-70-4) in human plasma. The analyte the internal standard nimodipine (CAS 66085-59-4) were extracted from plasma samples by n-hexane-isopropanol (95:5, v/v), analyzed on a commercially available column interfaced with mass spectrometer. Positive atmospheric pressure...

13-hexyl-berberine hydrochloride (HB-13) is a derivative from berberine which finds widespread applications in the treatment of infectious pathogens including fungi, bacteria, parasites and viruses. As our continuing efforts for herpes simplex virus (HSV), we studied topical delivery safety HB-13 gel formulation (0.5%) pig model. Our studies demonstrated maximal concentration was 2.51 µg/mL, more than half inhibitory (IC50) as previously reported. In addition, there no sign irritation or...

A selective and sensitive method employing high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-mass spectrometry is developed validated for the determination of emedastine difumarate in human plasma. With naphazoline hydrochloride as internal standard, extracted from plasma with ethyl acetate. The organic layer evaporated, residue redissolved mobile phase. An aliquot 10 µL chromatographically analyzed on a prepacked Phenomenex Luna 5u CN 100A (150 × 2.0-mm i.d.)...

Abstract A rapid, sensitive and specific high‐performance liquid chromatography–electrospray ionization mass spectrometry (LC‐ESI‐MS) method has been developed validated for the determination of oxatomide in human plasma. Flunarizine hydrochloride was employed as internal standard (IS). The analytes were chromatographically separated on a Shimadzu Shim‐pack VP‐ODS C 18 column (250 × 2.0 mm i.d.) with mobile phase consisting methanol aqueous ammonium acetate solution (10 m , pH 4.0; 85:15,...