A5035266557- Fibroblast Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Lysosomal Storage Disorders Research
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Protein Tyrosine Phosphatases
- Kruppel-like factors research
- Retinal Diseases and Treatments
- Biomarkers in Disease Mechanisms
- Retinal Development and Disorders
- Cardiovascular and exercise physiology
- Peptidase Inhibition and Analysis
- Sphingolipid Metabolism and Signaling
- Cellular transport and secretion
- Sirtuins and Resveratrol in Medicine
- Sports Performance and Training
- Cardiovascular Effects of Exercise
- Eosinophilic Disorders and Syndromes
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Retinopathy of Prematurity Studies
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
University of Brescia
2014-2024
General Department of Preventive Medicine
2006-2023
University of Naples Federico II
2017
Roche Pharma AG (Germany)
2014
KU Leuven
2010-2012
University of Bari Aldo Moro
2002-2012
Instituto de Química Médica
2012
Rega Institute for Medical Research
2010-2012
Istituti di Ricovero e Cura a Carattere Scientifico
2012
University of Milan
1988-2012
Abstract The chromosomal high mobility group box-1 (HMGB1) protein acts as a proinflammatory cytokine when released in the extracellular environment by necrotic and inflammatory cells. In present study, we show that HMGB1 exerts proangiogenic effects inducing MAPK ERK1/2 activation, cell proliferation, chemotaxis endothelial cells of different origin. Accordingly, stimulates membrane ruffling repair mechanically wounded monolayer causes sprouting three-dimensional fibrin gel. keeping with...
The contribution of polymorphonuclear neutrophils (PMN) to host defense and natural immunity extends well beyond their traditional role as professional phagocytes. In this study, we demonstrate that upon stimulation with proinflammatory stimuli, human PMN release enzymatic activities that, in vitro, generate bioactive angiostatin fragments from purified plasminogen. We also provide evidence these angiostatin-like fragments, comprising kringle domain 1 3 (kringle 1-3) plasminogen, are...
Neovascularization plays an important role in neoplasia and angioproliferative diseases. Two major modalities have been developed so far to affect neovascularization: its prevention by antiangiogenic compounds, immature vessel disruption vascular-targeting agents. <i>trans</i>-Resveratrol, found grapes wine, exerts antioxidant, antineoplastic, activities. Here, among various synthetic <i>trans</i>-resveratrol derivatives tested, 3,5,4′-trimethoxystilbene was agent 30 100 times more potent...
Angiogenesis is regulated by the balance between pro- and antiangiogenic factors extracellular matrix protein interactions. Fibroblast growth factor 2 (FGF2) a major proangiogenic inducer inhibited interaction with soluble pattern recognition receptor long pentraxin 3 (PTX3). PTX3 locally coexpressed its ligand tumor necrosis factor-stimulated gene-6 (TSG-6), secreted glycoprotein that cooperates in assembly. Here, we characterized effect of TSG-6 on PTX3/FGF2 FGF2-mediated...
Abstract Fibroblast growth factors ( FGFs ) exert autocrine/paracrine functions in prostate cancer by stimulating angiogenesis and tumour growth. Here dihydrotestosterone DHT up‐regulates FGF2 FGF8b production murine TRAMP‐C2 cells, activating a FGF ‐dependent autocrine loop of stimulation. The soluble pattern recognition receptor long pentraxin‐3 PTX3 acts as natural antagonist that binds via its N ‐terminal domain. We demonstrate recombinant protein the ‐derived pentapeptide Ac‐ ARPCA‐NH 2...
Tumor neovascularization (angiogenesis) is regarded as a promising target for anticancer drugs. Heparin binds to fibroblast growth factor-2 (FGF2) and promotes the formation of ternary complexes with endothelial cell surface receptors, inducing an angiogenic response. As novel strategy generate antiangiogenic substances exploiting binding FGF2 while preventing FGF receptor (FGFR) activation, sulfation gaps were generated along heparin chains by controlled alkali-catalyzed removal sulfate...
Fibroblast growth factor-2 (FGF2) is a potent angiogenic factor. Here, gene expression profiling of FGF2-stimulated microvascular endothelial cells revealed, together with prominent pro-angiogenic profile, pro-inflammatory signature characterized by the upregulation cytokine/chemokines and their receptors, cell adhesion molecules members eicosanoid pathway. Real-time quantitative PCR demonstrated early induction most FGF2-induced, inflammation-related genes. Accordingly, chick embryo...
The angiogenic basic fibroblast growth factor (FGF2) interacts with tyrosine kinase receptors (FGFRs) and heparan sulfate proteoglycans (HSPGs) in endothelial cells. Here, we report the FGF2 antagonist antiangiogenic activity of novel sulfated derivatives Escherichia coli K5 polysaccharide. polysaccharide was chemically inN- and/or O-position afterN-deacetylation. O-Sulfated andN,O-sulfated a low degree high sulfation compete heparin for binding to125I-FGF2 different potency. Accordingly,...
Fibroblast Growth Factor-2 (FGF2) is a major inducer of neovascularization (angiogenesis). Heparin activates FGF2 by favoring formation ternary complexes with its cellular receptors (FGFRs). Controlled 2-O-desulfation followed exhaustive periodate oxidation/borohydride reduction has been used to generate sulfation gaps within the prevalent heparin sequences, building-up arrays pentasulfated trisaccharides (PST, consisting 2-O-sulfated iduronic acid flanked two N,6-disulfated glucosamines)...
Purpose.: Pharmacologic control of neovascularization is a promising approach for the treatment retinal angiogenesis. UPARANT, an inhibitor urokinase-type plasminogen activator receptor (uPAR), inhibits VEGF-driven angiogenesis in vitro and vivo. This study investigates first time effectiveness UPARANT counteracting pathologic retina. Methods.: Murine fragments mouse model oxygen-induced retinopathy (OIR) were used. In mice with OIR, UPARANT-treated retinas analyzed avascular area...
Abstract Angiogenesis is target for antineoplastic and chemopreventive therapies. The natural phytoalexin resveratrol found in grapes red wine as cis trans stereoisomers. trans-Resveratrol shows antiangiogenic activity, but its mechanism of action not fully elucidated. Recently, trans-resveratrol has been shown to interact with the β3 integrin subunit, raising possibility that inhibition endothelial αvβ3 function may concur angiosuppressive activity. To get novel insights about activity...
Abstract Chemokines influence tumor growth directly or indirectly via both angiogenesis and tumor-leukocyte interactions. Platelet factor-4 (CXCL4/PF-4), which is released from α-granules of activated platelets, the first described angiostatic chemokine. Recently, it was found that variant CXCL4/PF-4 (CXCL4L1/PF-4var) could exert a more pronounced antitumoral effect than CXCL4/PF-4. However, molecular mechanisms activities PF-4 forms remain partially elusive. Here, we studied biological...
Globoid cell leukodystrophy (Krabbe disease) is a neurological disorder of infants caused by genetic deficiency the lysosomal enzyme β-galactosylceramidase leading to accumulation neurotoxic metabolite 1-β-d-galactosylsphingosine (psychosine) in central nervous system. Angiogenesis plays pivotal role physiology and pathology brain. Here, we demonstrate that psychosine has anti-angiogenic properties causing disassembling endothelial actin structures at micromolar concentrations as found brain...