A5017447026- Virus-based gene therapy research
- bioluminescence and chemiluminescence research
- Bee Products Chemical Analysis
- Healthcare and Venom Research
- Protease and Inhibitor Mechanisms
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Insect and Pesticide Research
- Metabolism and Genetic Disorders
- Prostate Cancer Treatment and Research
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Cholesterol and Lipid Metabolism
- Cancer Cells and Metastasis
- Iron Metabolism and Disorders
- Cancer Immunotherapy and Biomarkers
- Microbial Natural Products and Biosynthesis
- Helminth infection and control
- Trace Elements in Health
- Advanced Fluorescence Microscopy Techniques
- Radiopharmaceutical Chemistry and Applications
- Marine Sponges and Natural Products
- Sphingolipid Metabolism and Signaling
- Neuropeptides and Animal Physiology
- Bioactive Compounds and Antitumor Agents
University of Otago
2014-2021
Epic Sciences (United States)
2015-2016
Stanford Medicine
2015
Queen Mary University of London
2007-2010
Ludwig Cancer Research
2010
University of Canterbury
2004-2008
Cancer Research UK
2006-2007
Linköping University
2004-2006
While programmed death 1 (PD-1) and death-ligand (PD-L1) checkpoint inhibitors have activity in a proportion of patients with advanced bladder cancer, strongly predictive prognostic biomarkers are still lacking. In this study, we evaluated PD-L1 protein expression on circulating tumor cells (CTCs) isolated from muscle invasive (MIBC) metastatic (mBCa) cancer explore the value CTC clinical outcomes. Blood samples 25 MIBC or mBCa were collected at UCSF shipped to Epic Sciences. All nucleated...
The introduction of apo-ferritin or the iron chelator DFO (desferrioxamine) conjugated to starch into lysosomal compartment protects cells against oxidative stress, rupture and ensuing apoptosis/necrosis by binding intralysosomal redox-active iron, thus preventing Fenton-type reactions peroxidation membranes. Because up-regulation MTs (metallothioneins) also generates enhanced cellular resistance including X-irradiation, were found be capable in an acidic reducing lysosomal-like environment,...
Mesenchymal stem cells (MSCs) form part of tumor stroma, and are typically considered to be pro‐tumorigenic, promoting continuing growth metastasis. Here, we describe a mechanism by which MSCs may anti‐tumorigenic, through inhibition breast cancer cell migration invasion, an important MDA‐MB‐231 T47D were co‐cultured in Transwell insert above or MSC conditioned media their invasion Matrigel measured. The was found inhibit movement. TIMP‐1 TIMP‐2, inhibitors matrix metalloproteinases (MMPs),...
Increasing evidence supports a role of cellular iron in the initiation and development atherosclerosis. We others reported earlier that iron-laden macrophages are associated with LDL oxidation, angiogenesis, nitric oxide production apoptosis atherosclerotic processes. Here we have further studied perturbed metabolism macrophages, their interaction lipoproteins origin accumulation human atheroma. In both early advanced atheroma lesions, hemoglobin ferritin correlated macrophage-rich areas....
The microtubule-stabilizing drug paclitaxel has activity in relapsed ovarian cancer. dl922-947, an oncolytic adenovirus with a 24-bp deletion E1A CR2, replicates selectively within and lyses cells dysregulated Rb pathway efficacy In the aggressive A2780CP xenograft, combination treatment weekly dl922-947 significantly greater than either alone can produce complete tumor eradication some animals. We investigated mechanisms of paclitaxel's synergy host-cell microtubule network is grossly...
Honey has been shown to have a range of therapeutic effects in humans, with anti-inflammatory and anti-bacterial among those previously characterised. Here, we examine the possibility New Zealand thyme, manuka honeydew honeys, their major sugar phenolic components, reducing development metastatic cancer. Their activity was examined vitro, PC3 DU145 prostate cancer cell lines, through measuring compounds' on characteristics migration, invasion adhesion. First, compounds gallic acid, caffeic...
Pinnatoxin G is a cyclic imine neurotoxin produced by dinoflagellates that has been reported in shellfish. Like other members of the pinnatoxin family, it shown to have its effects via antagonism nicotinic acetylcholine receptors, with preferential binding α7 subunit often upregulated cancer. Because increased activity receptors contributes growth and resistance apoptosis, effect on cancer cell viability was tested. In panel six lines, all types lost viability, but HT29 colon LN18 U373...
Monocytic cell lines have been extensively used to characterize and model various features of the atherogenic process. We found striking differences in apoptotic pathways U937 cells THP‐1 exposed copper‐oxidized LDL. While phosphatidylserine exposure occurred both lines, caspase activation was only apparent cells. OxLDL caused activity decrease below that seen untreated cells, this corresponded with a loss intracellular thiols. In conclusion, oxLDL did not trigger conventional apoptosis...
Abstract The adenoviral E1A CR2 mutant dl922-947 has potent activity in ovarian cancer. We have used Renilla luciferase bioluminescence imaging to monitor viral expression and replication [18F]fluorothymidine positron emission tomography ([18F]FLT-PET) quantify the of vivo. created dlCR2 Ren, with same deletion as gene from reniformis downstream E1. Light emitted s.c. i.p. IGROV1 carcinoma xenografts was measured following treatment Ren. Mice bearing were injected 2.96 3.7 MBq [18F]FLT 48...
Cytotoxic amides have been isolated from the fruits of endemic New Zealand medicinal plant kawakawa, <i>Macropiper excelsum</i> (Piperaceae). The main amide was piperchabamide A and this is first report rare compound outside genus <i>Piper</i>. Eleven other were purified including two new compounds with unusual 3,4-dihydro-1(2H)-pyridinyl group. fully characterized by 2D NMR spectroscopy, which showed a slow exchange between rotamers about bond, they chemically synthesized. In view antitumor...
The use of radiolabeled antibodies that are able to target primary tumors as well metastatic tumor sites with minimal reactivity normal tissues is a promising approach for treating pancreatic cancer. In this study, the integrin αvß5 studied diagnosis and potential therapy human cancer by using murine monoclonal antibody (mAb) 14C5. Biopsy specimens from were examined expression αvß5. cell line Capan-1 was used test in vitro targeting potency mAb 14C5 labeled 125/131-iodine 111-indium....
The synthesis of anithiactin A has been achieved in four steps. Several closely related analogues were synthesised and their biological activity against colon breast cancer cell lines evaluated. Anithiactin was found not to be cytotoxic even at a high concentration (100 μM); however, two 4-substituted phenyl thiazoles moderately 10 μM. Based on these results, 4-substitution the group appears critical for cytotoxicity. However, exact electronic structural requirements are unclear.
Mesenchymal stem cells home to the tumour stroma from bone marrow, influencing early development and metastasis. have been shown be promising vehicles for cancer therapy due this tropism sites, their immunoprivileged status, easy extraction marrow facile transfection. used deliver gene in form of cytokines or adenovirus as part prodrug strategies. However, order these agents tested clinical trials, biology mesenchymal must further investigated. At present there is variation between research...