A5051893171- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Metabolism, Diabetes, and Cancer
- Biochemical Analysis and Sensing Techniques
- Pancreatic function and diabetes
- RNA modifications and cancer
- Nutrition, Genetics, and Disease
- Diabetes and associated disorders
- Biochemical effects in animals
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- FOXO transcription factor regulation
- Antioxidant Activity and Oxidative Stress
- Autophagy in Disease and Therapy
- Dermatology and Skin Diseases
- PARP inhibition in cancer therapy
- RNA and protein synthesis mechanisms
- Sphingolipid Metabolism and Signaling
- Adipokines, Inflammation, and Metabolic Diseases
- Ion Channels and Receptors
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
Beth Israel Deaconess Medical Center
2019-2024
Harvard University
2019-2024
Seoul National University
2019
Ansan University
2019
Dongguk University
2013-2018
MicroRNAs have been shown to play an important role in insulin signaling but their biological function resistance induced by saturated fatty acids (SFA) remains largely unknown. Here, we report that SFA palmitate and high fat diet (HFD) significantly increase expression of miR‐29a myocytes. targets IRS‐1 3'UTR directly represses at the translational level. Furthermore, ectopic impairs glucose uptake myocytes through a substantial decrease IRS‐1. These findings suggest up‐regulation is...
Scope Obesity increases intracellular lipid accumulation in key tissues or organs, which often leads to metabolic dysfunction and insulin resistance. Diets rich saturated fatty acid (SFA) exacerbate obesity hepatic steatosis, accentuate the risk of resistance type 2 diabetes (T2DM). Although microRNAs (miRNAs) play a critical role regulation gene expression, implication obesity‐induced miRNAs disorders particularly development is largely unknown. Here, we investigated miR‐15b, induced by SFA...
MicroRNAs (miRNAs) play an important role in insulin signaling and secretion, but the of miRNAs association between obesity hepatic resistance is largely unknown. This study reports that saturated fatty acid (SFA) high fat diet (HFD) significantly induce miR‐195 expression hepatocytes, receptor (INSR), not substrate‐1 (IRS‐1), a direct target miR‐195. Furthermore, ectopic suppresses INSR, thereby impairing cascade glycogen synthesis HepG2 cells. These findings suggest dysregulation by SFA...
Obesity is defined as the excessive accumulation of body fat that ultimately leads to chronic metabolic diseases. Diets rich in saturated fatty acids (SFA) exacerbate obesity and hepatic steatosis, which increase risk insulin resistance type 2 diabetes (T2DM). Although microRNAs (miRNAs) play an important role a range biological processes, implications SFA-induced miRNAs dysregulation, particularly pathogenesis resistance, are not well understood. This study investigated miR-96, induced...
Low-density lipoprotein receptor-related protein-1 (LRP1) regulates energy homeostasis, blood-brain barrier integrity, and metabolic signaling in the brain. Deficiency of LRP1 inhibitory gamma-aminobutyric acid (GABA)ergic neurons causes severe obesity mice. However, impact on memory function cognition context is poorly understood. Mice lacking GABAergic (Vgat-Cre; LRP1loxP/loxP) underwent behavioral tests for locomotor activity motor coordination, short/long-term spatial memory, fear...
Spirodela polyrhiza (L.) Schleid. (Lemnaceae), Spirodelae Herba (SH), has been known to relieve inflammation, urticaria and skin symptoms including pruritus, eczema rash.The effects of SH extract on two calcium ion channels, Orai1 TRPV3, their potential as novel therapeutics for atopic dermatitis (AD) were investigated. The regulatory role mast cell degranulation was evaluated.The dried leaves extracted by 70% methanol. Effects (100 μg/mL) in an HEK293T line overexpressing human or TRPV3...
A previous study indicated a causal link between certain miRNAs induced by obesity and the development of hepatic insulin resistance type 2 diabetes. Here we provide accompanying data collected using Affymetrix GeneChip microarrays to identify changes in expression liver mice fed high fat diet (HFD). Differentially expressed microRNA analyses HFD-fed revealed range upregulated (>1.5-fold) or downregulated (<0.5-fold) miRNAs. Among those miRNAs, silico target analysis, such as TargetScan,...
Low-density lipoprotein receptor-related protein-1 (LRP1) regulates energy homeostasis, and its dysfunction has been linked to cognitive decline, dementia, Alzheimer’s disease. However, the impact of LRP1 in inhibitory neurons on memory function cognition obesity remains unexplored. Mice lacking GABAergic (Vgat-Cre; LRP1loxP/loxP) are subjected conduct behavioral tests locomotor activity motor coordination, short/long-term spatial memory, fear learning/memory. We evaluated relationships...
Low-density lipoprotein receptor-related protein 1 (LRP1) is a member of LDL receptor family that plays key role in systemic glucose and lipid homeostasis. LRP1 also regulates energy balance the hypothalamus by mediating leptin's anorexigenic action, although underlying neurocircuitry involved still unclear. Because GABAergic neurons are major mediator hypothalamic leptin we studied action using mice lacking Vgat- or AgRP-expressing (Vgat-Cre;
Dietary fats rich in saturated fatty acid (SFA) increase the risk of metabolic diseases, and certain microRNAs (miRNAs) dysregulated by SFA are associated with pathogenesis insulin resistance type 2 diabetes. A previous study found that miR-195 is increased impairs hepatic signaling through suppression INSR (Yang et al., 2014) [1]. This article reports accompanying data to determine effect on expression PEPCK, a key player gluconeogenesis. The transfection HepG2 hepatocytes was mRNA protein...
Obesity and metabolic diseases are closely associated with insulin resistance. Obesity-induced miRNAs also considered to be potential contributors the development of resistance type 2 diabetes. Previously, expression miR-1271 was reported upregulated in liver diet-induced obese mice (Yang et al., 2016) [1]. In this data article, multiple silico analysis predicted FOXO1 gene a direct target miR-1271. Dual luciferase reporter showed that suppressed by binding 3′UTR. The overexpression reduced...
Diets containing a high saturated fatty acid (SFA) increase the risk of metabolic diseases, and microRNAs (miRNAs) induced by SFA have been implicated in pathogenesis insulin resistance type 2 diabetes. In previous report, miR-96 is found to be upregulated involved suppression signaling intermediates, leading hepatocytes (Yang et al., 2016) [1]. This article presents accompanying data collected from L6-GLUT4myc myocytes determine effects on skeletal muscle cells. The transfection decreased...
This article reports the data for effects of C1q tumor necrosis factor α-related protein isoform 5 (CTRP5) on palmitate-induced apoptosis in myocytes. The obtained from vitro cultured myocytes shows that cellular treatment with globular domain CTRP5 (gCTRP5) significantly inhibits MTT reduction, caspase-3 activation, and DNA fragmentation a time-dependent manner. presented this also AraA, an inhibitor AMPK, almost completely abolished protective effect gCTRP5 induced by palmitate...
Certain microRNAs (miRNAs) targeting the molecules in insulin signaling cascades are dysregulated by saturated fatty acids (SFA), which can lead to resistance and type 2 diabetes. This article reports accompanying data collected using miRNAs microarrays identify changes miRNA expression HepG2 cells treated with SFA palmitate. Differentially expressed analyses showed that a range of upregulated (>1.5-fold) or downregulated (<0.5-fold) miRNAs. Further extensive insights into implications...
Leptin is an adipocyte-derived satiety factor that suppresses food intake and increases energy expenditure by reaching anorexigenic neurons in the brain. While leptin typically interacts with receptor (LepR), evidence reveals a high binding affinity of to choroid plexus (ChP), distinct brain barrier mediates peripheral-brain signal communication, LepR-deficient db/db mice obese Zucker rats. This suggests ChP may be major site for transport through specific receptors other than LepR. Here, we...
Abstract Altered hepatic glucose fluxes are critical during the pathogenesis of type 2 diabetes. G protein-coupled receptors represent important regulators production. Recent studies have shown that hepatocytes express GPCRs can couple to 12/13 , a subfamily heterotrimeric proteins has attracted relatively little attention in past. Here we show, by analyzing several mutant mouse strains, selective activation hepatocyte signaling leads pronounced hyperglycemia and this effect involves...
Adipocyte-derived leptin enters the brain to exert its anorexigenic action, yet transport mechanism is poorly understood. Here we report that LRP1 (low-density lipoprotein receptor-related protein-1) mediates of across blood-CSF barrier in Foxj1 expressing cells highly enriched at choroid plexus (ChP), coupled with short-form receptor, and deletion from ependymocytes ChP leads resistance hyperphagia, causing obesity. Thus, epithelial a principal regulator brain.
ABSTRACT Objective Low-density lipoprotein receptor-related protein-1 (LRP1) regulates energy homeostasis, blood-brain barrier integrity, and metabolic signaling in the brain. Loss of LRP1 from inhibitory gamma-aminobutyric acid (GABA)ergic neurons causes severe obesity mice. Its dysfunction has been associated with cognitive decline, dementia, Alzheimer’s disease. However, impact on memory function cognition context is poorly understood. Methods Mice lacking GABAergic ( Vgat-Cre; loxP/loxP...
ABSTRACT Objective Melanocortin action is essential for the maintenance of energy homeostasis. However, knowledge signaling mechanism(s) that mediates effect melanocortin remains incomplete. Methods ROCK1 a key regulator balance in hypothalamus. To explore role anorexigenic melanocortin, we deleted MC4R neurons mice. Next, studied metabolic effects neuron-specific ROCK1-deficiency and following treatment with α-melanocyte-stimulating hormone (MSH). Results Here show α-MSH increases...