A5088016065- Platelet Disorders and Treatments
- Blood groups and transfusion
- Blood disorders and treatments
- Heparin-Induced Thrombocytopenia and Thrombosis
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Antiplatelet Therapy and Cardiovascular Diseases
- Autoimmune Bullous Skin Diseases
- Immunodeficiency and Autoimmune Disorders
- Diabetes Treatment and Management
- Complement system in diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hemophilia Treatment and Research
- Genomic variations and chromosomal abnormalities
- Cell Adhesion Molecules Research
- Blood Coagulation and Thrombosis Mechanisms
- Renal Diseases and Glomerulopathies
- Bone health and treatments
- Osteoarthritis Treatment and Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Protease and Inhibitor Mechanisms
- Bone Metabolism and Diseases
- Gastrointestinal Bleeding Diagnosis and Treatment
- Obstructive Sleep Apnea Research
- Bone and Dental Protein Studies
- Hemostasis and retained surgical items
Versiti Blood Center of Wisconsin
2014-2024
Washington University in St. Louis
2023
Medical College of Wisconsin
2002-2016
Zimmerman Associates (United States)
2013
Children's Hospital of Wisconsin
2002-2006
University of Miami
2001
Pfizer (United States)
1999
Howard Hughes Medical Institute
1998
Weatherford College
1998
Summary Bleeding Assessment Tools (BATs) have been developed to aid in the standardized evaluation of bleeding symptoms. The Vicenza Questionnaire (BQ), published 2005, established a common framework and scoring key that has undergone subsequent modification over years, culminating publication ISTH‐BAT 2010. Understanding normal range scores is critical when assessing utility BAT. Within context Merging Project, bioinformatics system was created facilitate merging legacy data derived from...
BACKGROUND von Willebrand factor (VWF) is a multimeric protein that binds platelets and collagen, facilitating hemostasis at sites of vessel injury. Measurement VWF multimer distribution critical for diagnosis variant disease (VWD), particularly types 2A 2B, but the typical measurement by gel electrophoresis technically difficult time-consuming. A comparison collagen binding (VWF:CB) was performed to evaluate utility VWF:CB as diagnostic test. METHODS Participants were enrolled in Zimmerman...
BackgroundBleeding assessment tools are key screening tests used in the evaluation of patients with suspected inherited bleeding disorders. The International Society on Thrombosis and Haemostasis-Scientific Standardization Committee endorsed Bleeding Assessment Tool (ISTH-BAT) has differing reference ranges for adult males (0-3), females (0-5), children (0-2), reflecting symptoms exposure to hemostatic challenges these healthy population subgroups. Age is known markedly impact score...
Abstract Radiographic mammary microcalcifications are one of the most pertinent diagnostic markers breast cancer. Breast tissue calcification in form calcium hydroxyapatite (HA) is strongly associated with malignant disease. We tested hypothesis that HA may exert biological effects on surrounding cells, thereby facilitating cancer progression. Our findings showed crystals enhanced mitogenesis cell lines MCF‐7 and Hs578T also normal human epithelial cells. were found to upregulate production...
<h3>OBJECTIVE</h3> To determine the ability of basic calcium phosphate (BCP) crystals to induce (<i>a</i>) mitogenesis, matrix metalloproteinase (MMP)-1, and MMP-13 in human osteoarthritic synovial fibroblasts (HOAS) (<i>b</i>) cultured porcine articular chondrocytes. <h3>METHODS</h3> Mitogenesis HOAS was measured by [<sup>3</sup>H]thymidine incorporation assay counts cells monolayer culture. MMP messenger RNA (mRNA) accumulation determined either northern blot analysis or reverse...
Synovial fluid basic calcium phosphate (BCP) crystals are markers of severe joint degeneration in osteoarthritis. BCP cause mitogenesis articular cells and stimulate matrix metalloprotease production, thus promoting degradation tissues. Previous work suggested that crystal-induced cell activation required intracellular crystal dissolution, induction proto-oncogene expression, signal transduction pathways involving protein kinase C mitogen-activated kinases. Here we further elucidate the...
Key Points In children undergoing tonsillectomy, low VWF levels did not predict surgical bleeding in the absence of major history. Children have lower than adults and these do appear to be associated with bleeding.
The von Willebrand factor (VWF) propeptide (VWFpp) is critical for the targeting of VWF multimers to storage granules. VWFpp alone efficiently navigates pathway in AtT-20 and endothelial cells chaperones mature granules when two proteins are expressed cis or trans . To further define role granular sorting, we examined its ability sort an unrelated protein, C3α into regulated secretory pathway. Chimeric constructs α-chain C3 were developed. protein did not cells. expression resulted but no...
This article will discuss the diagnosis and management of von Willebrand disease (VWD) in United States results from Zimmerman Program, a national study VWD. An algorithm is presented to show how we currently approach diagnostic testing for VWD, including potential replacement ristocetin cofactor assay with new factor (VWF)-GPIb binding assay. Results Program type 1 cohort are presented, findings that genetic defects VWF gene most common levels <30 IU/dL, but bleeding symptoms were present...