Moringa oleifera (MO) is an important plant for traditional medicine. The present study aimed to identify the MO active phytochemical compounds their ability against inflamed macrophages. An ethyl acetate extract fraction of was fractionation by flash column chromatography. Human macrophages were stimulated Lipopolysaccharide and then treated with fractions examine anti-inflammatory activity cellular mechanism. analyzed liquid chromatography electrospray ionization quadrupole time-of-flight...

Triple negative breast cancer is one of the most aggressive type with abilities early metastasis and chemoresistance. The tropical plant Citrus hystrix DC. has been reported to promote many biological activities including anticancer. However, effect C. against triple not yet identified. This study aimed evaluate anticancer properties leaf extract its bioactive constituents citronellol citronellal MDA-MB-231 cell line. leaves were powdered sequentially macerated. in vitro effects extracts,...

Squamous cell carcinoma is the most common type of head and neck cancer worldwide. Radiation chemotherapy are general treatments for patients; however, these remedies can have adverse side effects tumours develop drug resistance. Effective still require improvement patients. Here, we investigated anti-cancer effect Moringa oleifera (MO) Lam. leaf extracts their fractions, 3-hydroxy-β-ionone on SCC15 line. were treated with without MO fractions. MTT assay was used to determine viability...

Liver fibrosis, a consequence of chronic liver damage or inflammation, is characterized by the excessive buildup extracellular matrix components. This progressive condition significantly raises risk severe diseases like cirrhosis and hepatocellular carcinoma. The lack approved therapeutics underscores urgent need for novel anti-fibrotic drugs. Hepatic stellate cells (HSCs), key players in fibrogenesis, are promising targets drug discovery. study investigated potential Citrus hystrix DC. (KL)...

Liver fibrosis, characterized by excessive extracellular matrix deposition, is driven activated hepatic stellate cells (HSCs). Due to the limited availability of anti-fibrotic drugs, research on therapeutic agents continues. Here we have investigated

Pythiosis is a rare infectious disease caused by Pythium insidiosum, which typically occurs in tropical and subtropical regions. The high mortality rate may be consequence of the lack diagnosis. objective this study was to evaluate reliability new single-tube nested PCR for detection P. insidiosum DNA. A total 78 clinical isolates various fungi bacteria, 106 specimens 80 simulated positive blood samples were tested. developed primer pairs CPL6-CPR8 YTL1-YTR1 are located on 18S subunit rRNA...

A new coumarin, scataccanol (1) and 10 known compounds were isolated from the fruits of Scaevola taccada (Gaertn.) Roxb. All evaluated for antifungal activity against Pythium insidiosum. Compounds 5 7 showed strong with minimum inhibitory concentration values μg/mL, respectively. Structural determination all was accomplished by 1D 2D-NMR, IR MS.

Citrus hystrix DC. (CH) is found in many countries Southeast Asia. This plant has been reported for anti-microbial, anti-cancer and anti-inflammatory bioactivities. However, the anti-inflammasome properties of leaves remain poorly understood. study aimed to investigate effect CH on NLRP3 NF-κB signaling pathways. were sequentially extracted using hexane, ethyl acetate 95% ethanol give three crude extracts. An active compound, lupeol was fractionated from ethanolic extract chromatographic...

Liver fibrosis, a consequence of chronic liver damage or inflammation, is characterized by the excessive buildup extracellular matrix components. This progressive condition significantly raises risk severe diseases like cirrhosis and hepatocellular carcinoma. The lack approved therapeutics underscores urgent need for novel anti-fibrotic drugs. Hepatic stellate cells (HSCs), key players in fibrogenesis, are promising targets drug discovery. study investigated potential Citrus hystrix DC. (KL)...

Smokers have high plaque accumulation that initiates gingival inflammation and progresses to periodontitis. Thus, oral hygiene control microbial formation is an effective method of preventing gingivitis. Medicinal plants such as Moringa oleifera Lam. (MO) Cyanthillium cinereum (Less.) H. Rob. (CC) anti-inflammatory effect might improve health in smokers. This study evaluated the MO leaf CC extracts using lozenges a combination + on gingivitis volunteer Lozenges consisting were developed...

Oral hygiene and control of microbial plaque biofilm formation are effective methods for preventing gingivitis. Mouthwashes containing leaf extracts the medicinal plants Citrus hystrix DC. (KL), Moringa oleifera Lam. (MO) Azadirachta indica A. Juss. (NE) were assessed oral healthcare gingivitis adjunctive treatment. Three types mouthwash developed; KL, a combination KL MO (KL + MO), NE NE). The mouthwashes tested in vivo on 47 subjects with who allocated into five groups as (i) placebo, (ii)...

Clinical yeast isolates belonging to Candida pelliculosa, utilis and fabianii are difficult distinguish in a routine mycology laboratory using common biochemical tests. The aims of this study were determine the prevalence C. clinical samples compare their minimum inhibitory concentrations (MICs) systemic antifungals. Two hundred forty-eight obtained from eight large hospitals Czech Republic included study. Identification was performed biochemically ID 32C kit by MALDI-TOF MS. MICs determined...

Two new compounds, dalpulanone (1) and 2-hydroxyisomucronustyrene (2), were isolated from the stems of Dalbergia stipulacea. Fourteen known compounds (3–16) also isolated. The chemical structures all elucidated using spectroscopic methods, including 1D-NMR, 2D-NMR, MS IR data. Compound 4 displayed strongest antifungal activity against Pythium insidiosum had higher than amphotericin-B standard.

Aims: Screening of bacterial flora for strains producing metabolites with inhibitory effects on the human pathogenic oomycete Pythium insidiosum. Separation and characterization extracts from Pseudomonas stutzeri anti-Pythium activity. Search genes effect within genome P. stutzeri. Methods: A total 88 were isolated water resources in northeastern Thailand. Two screening methods used to establish their One strain, ST1302 was randomly chosen, extract anti-P. insidiosum activity fractionated...

Candida albicans is a fungus that lives primarily on the mucosal surfaces of healthy humans, such as oral cavity, vagina, and gastrointestinal tract. This commensal organism can be controlled by other microbiota, while certain conditions increase risk C. outgrowth cause disease. Prevalence drug-resistant phenotype, well severity infection in immunocompromised patients, presents challenge for scientists to develop novel, effective treatment, prevention strategies. β-Citronellol an intriguing...

Hydroquinine is an organic alkaloid compound that exhibits antimicrobial activity against several bacterial strains including of both drug-sensitive and multidrug-resistant P. aeruginosa. Despite this, the effects hydroquinine on virulence factors in aeruginosa have not yet been characterized. We therefore aimed to uncover mechanism hydroquinine-sensitivity using high-throughput transcriptomic analysis. further confirmed whether inhibits specific RT-qPCR phenotypic At half minimum inhibitory...

Background: Pythiosis is a life-threatening disease caused by the fungus-like organism Pythium insidiosum. It causes in both animals and humans. Amphotericin B antifungal less effective because it lacks ergosterol, drug target cell membrane. Objective: To evaluate antimicrobial susceptibility test of P. insidiosum isolated from human pythiosis disc diffusion method. Methods: The method was tested against 10 clinical strains Antimicrobial drugs comprise 8 antibiotics (chloramphenicol,...

Moringa leaves provide numerous health benefits due to their anti-inflammatory properties. This study presents the first evidence that endothelial cell inflammation can potentially be ameliorated by moringa leaf extract. Here, we established an experimental human blood vessel model of using EA.hy926 cells. TNF-α was added after pre-treating cells with crude extract from oleifera Lam., a constituent fraction extract, and bioactive component 3-hydroxy-β-ionone. The active ingredient...

Chemical investigation of Dalbergia cultrata Grah. stems growing in Thailand resulted the isolation and characterization 14 known compounds, including tectorigenin (1), calycosin (2), pratensein (3), afromosin (4), irilin D (5), biochanin A (6), daidzein (7), formononetin (8), 8-O-methylretusin (9), 7,3′-dihydroxy-5′-methoxyisoflavone (10), (3S)-sativanone (11), (3S)-violanone (12), erycibenin (13) isoliquiritigenin (14). Their structures were established mainly based on NMR spectroscopic...