A5003203172- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Ubiquitin and proteasome pathways
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Polyamine Metabolism and Applications
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Augmented Reality Applications
- Mobile and Web Applications
- PI3K/AKT/mTOR signaling in cancer
- Radiomics and Machine Learning in Medical Imaging
- Educational Games and Gamification
- Ocular Surface and Contact Lens
- Anatomy and Medical Technology
- Ocular Infections and Treatments
- COVID-19 Clinical Research Studies
- AI in cancer detection
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- Urinary Tract Infections Management
University of Portsmouth
2022-2024
Southampton Solent University
2019-2021
University of Gloucestershire
2021
UPMC Hillman Cancer Center
2018-2019
University of Sussex
2014-2019
University of Pittsburgh
2019
Abstract The error-free and efficient repair of DNA double-stranded breaks (DSBs) is extremely important for cell survival. RNA has been implicated in the resolution damage but mechanism remains poorly understood. Here, we show that miRNA biogenesis enzymes, Drosha Dicer, control recruitment factors from multiple pathways to sites damage. Depletion significantly reduces by both homologous recombination (HR) non-homologous end joining (NHEJ). required within minutes break induction,...
53BP1 plays multiple roles in mammalian DNA damage repair, mediating pathway choice and facilitating double-strand break repair heterochromatin. Although it possesses a C-terminal BRCT2 domain, commonly involved phospho-peptide binding other proteins, initial recruitment of to sites depends on interaction with histone post-translational modifications—H4K20me2 H2AK13/K15ub—downstream the early γH2AX phosphorylation mark damage. We now show that, contrary current models, 53BP1-BRCT2 domain...
Coordination of the cellular response to DNA damage is organised by multi-domain ‘scaffold’ proteins, including 53BP1 and TOPBP1, which recognise post-translational modifications such as phosphorylation, methylation ubiquitylation on other are themselves carriers regulatory signals. Here we show that checkpoint regulating S-phase entry controlled a phosphorylation-dependent interaction TOPBP1. BRCT domains TOPBP1 selectively bind conserved phosphorylation sites in N-terminus 53BP1. Mutation...
ABSTRACT Regulation of protein synthesis is crucial for cells to maintain viability and prevent unscheduled proliferation that could lead tumorigenesis. Exposure stress results in stalling translation, with many translation initiation factors, ribosomal subunits mRNAs being sequestered into granules or P bodies. This allows the re-programming machinery. Many aspects are regulated by post-translational modification. Several proteomic screens have identified factors as targets sumoylation,...
Machine intelligence has the potential to play a significant role in diagnosing, managing, and guiding treatment of disease, which supports rising demands on healthcare provide rapid accurate interpretation clinical data. The global pandemic caused by Severe Acute Respiratory Syndrome Coronavirus (SARSCoV-2) exposed need for data response an unprecedented burden system. A new challenge arisen – post-COVID syndrome or 'long COVID'. Symptoms can persist months following infection with...
DNA repair is critical for genome stability and maintained through conserved pathways. Traditional genome-wide mammalian screens are both expensive laborious. However, computational approaches circumvent these limitations a powerful tool to identify new factors. By analyzing the evolutionary relationships between genes in major pathways, we uncovered functional individual identified partners. Here ranked 17,487 coevolution with 6 distinct Direct comparison genetic homologous recombination or...
In recent years, development of new technologies designed to enhance user experience have accelerated, often being used in modern media such as films and games. Specifically, immersive experiences, virtual reality (VR) augmented (AR), redefined how digital can be delivered, encouraging us interact with explore our environment. Reciprocally, the power these has advanced, associated costs implement them decreased, making more cost-effective feasible deliver a variety settings. Despite cost...
P. aeruginosa is one of the most common bacteria causing contact lens-related microbial keratitis (CLMK). Previous studies report that disinfecting solutions were ineffective in preventing biofilm formation. Solutions containing novel natural agents may be an excellent alternative for reducing risk CLMK. Here, we investigate properties hydroquinine combination with multipurpose (MPSs) to prevent adhesion and We examined antibacterial, anti-adhesion, anti-biofilm hydroquinine-formulated MPSs...
SUMO is a small post-translational modifier, that attached to lysine residues in target proteins. It acts by altering protein-protein interactions, protein localisation and activity. chains can also act as substrates for ubiquitination, resulting proteasome-mediated degradation of the protein. removed from proteins one number specific proteases. The processes sumoylation desumoylation have well documented roles DNA metabolism maintenance chromatin structure. To further analyse role this...
Hydroquinine is an organic compound that closely related to quinine-derivative drugs and contains anti-malarial anti-arrhythmia activities. It has been also found in abundance some natural extracts possess antibacterial properties. However, there little evidence demonstrating the effect of hydroquinine. Therefore, we aimed investigate properties hydroquinine using broth microdilution methods. In addition, evaluated transcriptional responses P. aeruginosa hydroquinine-induced stress RNA...
Hydroquinine has antimicrobial potential with demonstrated activity against several bacteria, including multidrug-resistant (MDR) P. aeruginosa reference strains. Despite this, there is limited evidence confirming the antibacterial of hydroquinine clinical isolates and underlying mechanism action. Here, we aimed to investigate effect in strains using phenotypic susceptibility testing synergistic testing. In addition, examined inhibitory mechanisms MDR informatic-driven molecular docking...
Hydroquinine is an organic alkaloid compound that exhibits antimicrobial activity against several bacterial strains including of both drug-sensitive and multidrug-resistant P. aeruginosa. Despite this, the effects hydroquinine on virulence factors in aeruginosa have not yet been characterized. We therefore aimed to uncover mechanism hydroquinine-sensitivity using high-throughput transcriptomic analysis. further confirmed whether inhibits specific RT-qPCR phenotypic At half minimum inhibitory...
In recent years, development of new technologies designed to enhance user experience have accelerated, often being used in modern media including films and games. Specifically, immersive experiences such as Virtual Reality (VR) Augmented (AR) redefined how digital can be delivered, encouraging us interact with explore our environment. Reciprocally, the power these has advanced, associated costs implement them decreased making more cost-effective feasible deliver a variety settings. Despite...
Regulation of protein synthesis is fundamental importance to cells.It has a critical role in the control gene expression, and consequently cell growth proliferation.The this supported by fact that frequently upregulated tumor cells.The major point at which regulation occurs initiation stage.Initiation translation involves interaction several proteins form eIF4F complex, recognition mRNA subsequent recruitment 40S ribosomal subunit mRNA.This results formation 48S complex then scans for start...
The rise of multidrug resistance Pseudomonas aeruginosa highlights an increased need for selective and precise antimicrobial treatment. Drug efflux pumps are one the major mechanisms found in many bacteria, including P. aeruginosa. Detection genes using a polymerase chain reaction (PCR)-based system would enable detection aid clinical decision making. Therefore, we aimed to develop optimize novel method herein referred as "effluxR assay" multiplex digital PCR (mdPCR) mex pump strains....
Novelists and screenwriters have bombarded our imaginations with the idea of genetic engineering. From superhero origin stories to theme parks inhabited by dinosaurs, prospect re-writing code has inspired many raised ethical questions. The potential for these tools in medicine biological sciences prevent diseases is readily being explored. Recent successes include destruction simian immunodeficiency virus DNA from infected rhesus macaque monkeys (synonymous human virus). diverse power also...