A5065291326- Platelet Disorders and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiovascular Function and Risk Factors
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hemophilia Treatment and Research
- Inflammatory Biomarkers in Disease Prognosis
- Blood disorders and treatments
- Cardiac Imaging and Diagnostics
- Venous Thromboembolism Diagnosis and Management
- Acute Ischemic Stroke Management
- Monoclonal and Polyclonal Antibodies Research
- Complement system in diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Advanced biosensing and bioanalysis techniques
- Oral and gingival health research
- Ethics and Legal Issues in Pediatric Healthcare
- Sepsis Diagnosis and Treatment
- Autoimmune Bullous Skin Diseases
- Urinary Tract Infections Management
- Pharmacology and Obesity Treatment
- Inflammatory mediators and NSAID effects
- Cardiovascular Effects of Exercise
- Blood transfusion and management
- GDF15 and Related Biomarkers
Medical University of Vienna
2018-2025
Sapienza University of Rome
2020
Maastricht University
2020
Karolinska Institutet
2020
University Clinical Center Tuzla
2016
American University in Bosnia and Herzegovina
2011
2-deoxy-D-glucose is a synthetic glucose analog and potent inhibitor of glycolysis. Preclinical models demonstrated antiviral effects by inducing glucose-deprived state in cells, which interferes with viral replication. This was phase I, first-in-human, double-blind, randomized, placebo-controlled, dose-escalation trial single (cohort 1: 100µl one nostril, cohort 2: 200µl 3: both nostrils) multiple 4: four-times 200µl/day 5: nostrils, 6: six-times nostrils; all for seven days) ascending...
Von Willebrand factor (VWF) and VIII (FVIII) circulate in a noncovalent complex blood promote primary hemostasis clotting, respectively. A new VWF A1-domain binding aptamer, BT200, demonstrated good subcutaneous bioavailability long half-life non-human primates. This first-in-human, randomized, placebo-controlled, doubleblind trial tested the hypothesis that BT200 is well tolerated has favorable pharmacokinetic pharmacodynamic effects 112 volunteers. Participants received one of following:...
Abstract The effect of conventional anti-platelet agents is limited in secondary stroke prevention, and their effects are blunted under high shear stress the presence increased levels circulating von Willebrand factor (VWF). VWF critically involved thrombus formation at sites stenotic extracranial/intracranial arteries. A third generation anti-VWF aptamer (BT200) has been generated which could be useful for prevention. To characterize BT200 blood patients with large artery atherosclerosis...
Abstract Type 2B von Willebrand disease (VWD) is characterized by an increased binding affinity of factor (VWF) to platelet glycoprotein Ib. This can lead clearance high-molecular-weight (HMW) multimers and thrombocytopenia with a resulting moderate-severe bleeding phenotype. Rondoraptivon pegol (BT200) pegylated aptamer the A1 domain VWF novel mechanism action: it enhances VWF/factor VIII (FVIII) levels decreasing their clearance. To study potential benefit rondoraptivon in patients type...
Factor VIII (FVIII) circulates in a noncovalent complex with von Willebrand (VWF), the latter determining FVIII half-life. The VWF-binding aptamer rondaptivon pegol (BT200) increases plasma levels of VWF/FVIII healthy volunteers. This trial assessed its safety, pharmacokinetics, and pharmacodynamics hemophilia A. Nineteen adult patients (ages 20-62 years, 4 women) A (8 mild, 2 moderate, 9 severe) received subcutaneous injections pegol. After an initial fixed dose 3 mg on days 0 4, weekly...
Abstract Von Willebrand factor (VWF) plays a major role in arterial thrombosis. Antiplatelet drugs induce only moderate relative risk reduction after atherothrombosis, and their inhibitory effects are compromised under high shear rates when VWF levels increased. Therefore, we investigated the ex vivo of third-generation anti-VWF aptamer (BT200) before/after stimulated release. We studied concentration-effect curves BT200 had on activity, platelet plug formation (PFA), ristocetin-induced...
Background and aimsVon Willebrand factor (VWF) plays an important role in thrombogenesis mediates platelet adhesion particularly under high shear stress. Such conditions are generally found stenotic arteries can eventually cause myocardial infarction or stroke. We aimed to study whether levels of VWF antigen (VWF:Ag) predict future major adverse cardiovascular events (MACE) patients suffering from carotid artery stenosis.MethodsPatients with atherosclerotic disease defined by the presence...
Introduction:The commonest mitral regurgitation etiologies are degenerative (60%), rheumatic post-inflammatory, 12%) and functional (25%).Due to the large number of patients with acute MI, incidence ischaemic MR is also high.Ischaemic a complex multifactorial disease that involves left ventricular geometry, annulus, valvular/subvalvular apparatus.Ischaemic an important consequence LV remodeling after myocardial infarction.Research Objectives: The objective this study determine role...
Abstract Background von Willebrand factor (VWF) is crucial for arterial thrombosis and its plasma levels are increased in acute coronary syndromes (ACSs). The effects of conventional platelet inhibitors compromised by elevated VWF under high shear rates. BT200 a third-generation aptamer that binds inhibits the A1 domain human VWF. This article aims to study whether predictor mortality ACS patients potent P2Y12 blocker therapy examine inhibiting concentrations required inhibit samples with...
Defibrotide is approved for the treatment of sinusoidal obstruction syndrome after allogeneic stem cell transplantation. The exact mode action defibrotide unclear and human in vivo data are scarce. In this randomized, double blind, crossover trial we included 20 healthy volunteers. Four were randomized to receive placebo, while 16 received a 2 ng/kg bodyweight bolus lipopolysaccharide (LPS). Infusion 6.25 mg/kg or placebo was started one hour before injection LPS bolus. Plasma levels...
Abstract Osteoprotegerin (OPG) regulates bone metabolism by reducing the activation of osteoclasts, but may also be involved in blood vessel calcification and atherosclerosis. Within endothelial cells OPG is stored Weibel–Palade bodies (WPBs). Blood kinetics are essentially unknown. We aimed to assess these using two distinct vivo models; one after stimulation with desmopressin (DDAVP) another lipopolysaccharide (LPS). Both clinical trials were conducted at Department Clinical Pharmacology...
Recombinant tissue plasminogen activator (rt-PA) is a thrombolytic agent and essential in emergency medical care. Given recent supply shortages, the availability of biosimilar products an urgent need. However, biosimilarity trials are difficult to perform critically ill patients.
IntroductionHemodialysis patients (HDPs) exhibit extensive cardiovascular risk. The widely prescribed anti-platelet agent clopidogrel is metabolically activated by cytochrome enzymes, which may be impaired uremia and chronic low-grade inflammation, typically present in HDPs. We conducted a prospective multicenter study to investigate the pharmacokinetics pharmacodynamics of HDPs healthy volunteers (HVs).MethodsWe enrolled receiving long-term (75 mg) pantoprazole treatment (40 mg). Healthy...
Background: Von Willebrand Factor (VWF) is crucial for arterial thrombosis and its plasma levels are increased in acute coronary syndromes (ACS).Effects of conventional platelet inhibitors compromised by elevated VWF under high shear rates.BT200 a third generation aptamer that binds inhibits the A1 domain human VWF. Aims:i) To study whether predictor mortality ACS patients potent P2Y12 blocker therapy ii) examine effects inhibiting BT200 concentrations required to inhibit samples with...
Endocarditis is a rare and serious complication of brucellosis the main cause death in this pathology. Diagnosis requires high level attention, based on association epidemiological, clinical serological elements. Echocardiography plays crucial role early diagnosis, as well identifying predisposition to heart disease local complications typical This case study follows Brucella endocarditis involving aortic valve, 39-year-old male, diagnosed October 2010. Despite being given complete...
Abstract In primary hyperoxaluria type 1 excessive endogenous production of oxalate and glycolate leads to increased urinary excretion these metabolites. Although genetic testing is the most definitive preferred diagnostic method, quantification metabolites important for diagnosis evaluation potential therapeutic interventions. Current metabolite methods use laborious, technically highly complex expensive liquid, gas or ion chromatography tandem mass spectrometry, which are available only in...
interpret the experimental molecular unfolding profiles, we employed Langevin simulations and coarse-grained Self Organized Polymer model to complement experiments with dynamic force measurements in silico on a short fibrin protofibril fragment composed of four monomers upper lower strand (FP4/4).The force-extension profiles from AFM-based single-protofibril vitro were directly correlated.Results: The theoretical histograms peak forces revealed similar most probable forces, i.e. 230 pN...