A5012810644- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Sleep and Wakefulness Research
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Laser Applications in Dentistry and Medicine
- EEG and Brain-Computer Interfaces
- Circadian rhythm and melatonin
- Functional Brain Connectivity Studies
- Multisensory perception and integration
- Olfactory and Sensory Function Studies
- Medical and Biological Ozone Research
- stochastic dynamics and bifurcation
- Neuroscience and Music Perception
- Neurotransmitter Receptor Influence on Behavior
- Osteoarthritis Treatment and Mechanisms
- Endodontics and Root Canal Treatments
- Nerve injury and regeneration
- Blind Source Separation Techniques
- Bee Products Chemical Analysis
- Biochemical Analysis and Sensing Techniques
- Cell Image Analysis Techniques
- Receptor Mechanisms and Signaling
- Bone Tissue Engineering Materials
- Neuroblastoma Research and Treatments
University of Strathclyde
2015-2025
Hiroshima University
2020-2024
Hiroshima University Hospital
2023-2024
Jagiellonian University
2023
University of Lisbon
2023
Weatherford College
2023
Instituto de Medicina Molecular João Lobo Antunes
2023
Rutgers, The State University of New Jersey
2006-2011
Rutgers Sexual and Reproductive Health and Rights
2010
National Institute for Basic Biology
2002-2008
The responses of neocortical cells to sensory stimuli are variable and state dependent. It has been hypothesized that intrinsic cortical dynamics play an important role in trial-to-trial variability; the precise nature this dependence, however, is poorly understood. We show here auditory cortex urethane-anesthetized rats, population click can be quantitatively predicted on a trial-by-trial basis by simple dynamical system model estimated from spontaneous activity immediately preceding...
Within optogenetics there is a need for compact light sources that are capable of delivering with excellent spatial, temporal, and spectral resolution to deep brain structures. Here, we demonstrate custom GaN-based LED probe such applications the electrical, optical, thermal properties analyzed. The output power density emission spectrum were found be suitable stimulating channelrhodopsin-2, one most common light-sensitive proteins currently used in optogenetics. device produced high...
Controlling neural circuits is a powerful approach to uncover causal link between activity and behaviour. Optogenetics has been widely adopted by the neuroscience community as it offers cell-type-specific perturbation with millisecond precision. However, these studies require light delivery in complex patterns cellular-scale resolution, while covering large volume of tissue at depth vivo. Here we describe novel high-density silicon-based microscale light-emitting diode (μLED) array,...
Optogenetics has proven to be a revolutionary technology in neuroscience and advanced continuously over the past decade. However, optical stimulation technologies for vivo need developed match advances genetics biochemistry that have driven this field. In particular, conventional approaches illumination limitation on achievable spatio-temporal resolution. Here we utilize sapphire-based microscale gallium nitride light-emitting diode (µLED) probe activate neocortical neurons vivo. The probes...
The phase of low-frequency network activity in the auditory cortex captures changes neural excitability, entrains to temporal structure natural sounds, and correlates with perceptual performance acoustic tasks. Although these observations suggest a causal link between rhythms perception, it remains unknown how precisely they affect processes by which populations encode sounds. We addressed this question analyzing responses anesthetized rats using stimulus–response models. These models...
The spiking activity of cortical neurons is highly variable. This variability generally correlated among nearby neurons, an effect commonly interpreted to reflect the coactivation due anatomically shared inputs. Recent findings, however, indicate that correlations can be dynamically modulated, suggesting underlying mechanisms are not well understood. Here, we investigate hypothesis dominated by neuronal coinactivation: occurrence brief silent periods during which all in local network stop...
In vivo electrophysiology is the gold standard technique used to investigate sub-second neural dynamics in freely behaving animals. However, monitoring cell-type-specific population activity not a trivial task. Over last decade, fiber photometry based on genetically encoded calcium indicators (GECIs) has been widely adopted as versatile tool monitor vivo. this approach suffers from low temporal resolution. Here, we combine these two approaches both field potentials and mice. By developing an...
Neural activity is diverse, and varies depending on brain regions sleep/wakefulness states. However, whether astrocyte differs between states, there are differences in among remain poorly understood. Therefore, this study, we recorded intracellular calcium (Ca
Cortical circuits spontaneously generate coordinated activity even in the absence of external inputs. The character this depends on cortical state. We investigated how state affects organization spontaneous across layers rat auditory cortex vivo, using juxtacellular recording morphologically identified neurons and large-scale electrophysiological recordings. Superficial pyramidal cells putative fast-spiking interneurons were consistently suppressed during desynchronization. Pyramidal deep...
The brainstem plays a crucial role in sleep-wake regulation. However, the ensemble dynamics underlying sleep regulation remain poorly understood. Here, we show slow, state-predictive and state-dependent interactions between hippocampus mice. On timescale of seconds to minutes, populations can predict pupil dilation vigilance states exhibit longer prediction power than hippocampal CA1 neurons. sub-seconds, pontine waves (P-waves) are accompanied by synchronous firing neurons during both rapid...
Abstract The lateral habenula (LHb) is hyperactive in depression, and thus potentiating inhibition of this structure makes an interesting target for future antidepressant therapies. However, the circuit mechanisms mediating inhibitory signalling within LHb are not well-known. We addressed issue by studying neurons expressing either parvalbumin (PV) or somatostatin (SOM), two markers particular sub-classes neocortical neurons. Here, we find that both PV SOM expressed physiologically distinct...
Reactive oxygen species (ROS) induce oxidative stress in cells and are associated with various diseases, including autoimmune diseases. Ergothioneine (EGT) is a natural amino acid derivative derived from the ergot fungus has been reported to exhibit an effective antioxidant function many models of stress-related Recently, mutations OCTN1, membrane transporter EGT, have be rheumatoid arthritis. Therefore, we investigated chondrocyte-protective EGT using model stress-induced injury...
Abstract While evolutionary cortical expansion is thought to underlie the evolution of human cognitive capabilities, excessive developmental can lead megalencephaly, often found in neurodevelopmental disorders. Still, little known about how overproduction neurons during development affects processing and behavior later life. Here we show that superficial impairs auditory adult mice. We applied XAV939 overproduce excitatory development. XAV939-treated mice exhibited behavioral deficits...
Alzheimer's disease pathology typically manifests itself across multiple brain regions yet assessment at this scale in mouse models remains a challenge. This hinders the development of novel therapeutic approaches. Here we introduce fiber photometry approach to monitor amyloid freely behaving mice. We first demonstrated that flat fiber-based can detect signals under anesthesia after injecting blood-brain barrier permeable tracer, Methoxy-X04. The depth profile vivo fluorescent was correlated...
Animals can make faster behavioral responses to multisensory stimuli than unisensory stimuli. The superior colliculus (SC), which receives multiple inputs from different sensory modalities, is considered be involved in the initiation of motor responses. However, mechanism by information facilitates not yet understood. Here, we demonstrate that modulates competition among SC neurons elicit We conducted multiunit recordings rats performing a two-alternative spatial discrimination task using...
Abstract Deconvolution is a challenging inverse problem, particularly in techniques that employ complex engineered point-spread functions, such as microscopy with propagation-invariant beams. Here, we present deep-learning method for deconvolution that, lieu of end-to-end training ground truths, trained using known physics the imaging system. Specifically, train generative adversarial network images generated function system, and combine this unpaired experimental data preserve perceptual...
Imaging across length scales and in depth has been an important pursuit of widefield optical imaging. This promises to reveal fine cellular detail within a snapshot tissue sample. Current advances often sacrifice resolution through selective sub-sampling provide wide field view reasonable time scale. We demonstrate new avenue for recovering high-resolution images from sub-sampled data light sheet microscopy using deep-learning super-resolution. combine this with the use Airy beam achieve...
Abstract Neuronal activity can modify Alzheimer’s disease pathology. Overexcitation of neurons facilitate progression whereas the induction cortical gamma oscillations reduce amyloid load and improve cognitive functions in mouse models. Although previous studies have induced by either optogenetic activation parvalbumin-positive (PV+) or sensory stimuli, it is still unclear whether other approaches to induce also be beneficial. Here we show that PV+ basal forebrain (BF) increases burden,...